Strain identifier

BacDive ID: 15115

Type strain: Yes

Species: Streptomyces cyaneofuscatus

Strain history: KCC S-0364 <-- IFO 13190 <-- SAJ <-- ISP 5148 <-- INA 99/54.

NCBI tax ID(s): 66883 (species)

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General

@ref: 9348

BacDive-ID: 15115

DSM-Number: 40148

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, psychrophilic, Gram-positive

description: Streptomyces cyaneofuscatus DSM 40148 is an aerobe, spore-forming, psychrophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 66883
  • Matching level: species

strain history

@refhistory
9348<- E.B. Shirling, ISP <- T.P. Preobrazhenskaya, INA
67770KCC S-0364 <-- IFO 13190 <-- SAJ <-- ISP 5148 <-- INA 99/54.

doi: 10.13145/bacdive15115.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces cyaneofuscatus
  • full scientific name: Streptomyces cyaneofuscatus (Kudrina 1957) Pridham et al. 1958 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Streptomyces cavourensis subsp. washingtonensis
    20215Actinomyces cyaneofuscatus

@ref: 9348

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces cyaneofuscatus

full scientific name: Streptomyces cyaneofuscatus (Kudrina 1957) Pridham et al. 1958 emend. Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

@refgram stainconfidence
125439positive99
125438positive93.278

colony morphology

@refcolony colorincubation periodmedium used
19410Sand yellow10-14 daysISP 2
19410Sand yellow10-14 daysISP 3
19410Sand yellow10-14 daysISP 4
19410Green beige10-14 daysISP 5
19410Sand yellow10-14 daysISP 6
19410Brown beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium namecomplex name
19410noISP 2
19410yesISP 3Aerial Mycelium
19410yesISP 4Aerial Mycelium
19410noISP 5
19410noISP 6
19410noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9348GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19410ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19410ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19410ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19410ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19410ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19410ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperature
9348positivegrowth20
19410positiveoptimum28
67770positivegrowth28

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
125438aerobe93.756
125439obligate aerobe98.5

spore formation

  • @ref: 125438
  • spore formation: yes
  • confidence: 91.659

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan+energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68382beta-galactosidase+3.2.1.23
68382trypsin+3.4.21.4
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19410+++++++++++-+-++++-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19410++--+-+-+++

Isolation, sampling and environmental information

isolation

  • @ref: 9348
  • sample type: soil
  • geographic location: Daghestan
  • country: Russia
  • origin.country: RUS
  • continent: Europe

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_135.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_109;98_120;99_135&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AY999770
  • Sequence Identity:
  • Total samples: 4030
  • soil counts: 2441
  • aquatic counts: 286
  • animal counts: 1009
  • plant counts: 294

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
93481Risk group (German classification)
194101Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
9348Streptomyces cyaneofuscatus gene for 16S rRNA, partial sequence, strain: NBRC 13190AB1848601436nuccore66883
67770Streptomyces cyaneofuscatus strain JCM 4364 16S ribosomal RNA gene, partial sequenceAY9997701430nuccore66883
124043Streptomyces cyaneofuscatus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4364.D44081120nuccore66883

Genome sequences

  • @ref: 67770
  • description: Streptomyces cyaneofuscatus NRRL B-2570
  • accession: GCA_000718135
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 66883

GC content

@refGC-contentmethod
6777071.6genome sequence analysis
6777071fluorimetric

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes93.278no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.932no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes93.756no
125438spore-formingspore-formingAbility to form endo- or exosporesyes91.659no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno95.478no
125438motile2+flagellatedAbility to perform flagellated movementno87.5no
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes88.4
125439BacteriaNetmotilityAbility to perform movementno86.4
125439BacteriaNetgram_stainReaction to gram-stainingpositive99
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe98.5

External links

@ref: 9348

culture collection no.: DSM 40148, ATCC 19746, ATCC 23619, CBS 485.68, ETH 24190, IFO 13190, INA 99/54, ISP 5148, NBRC 13190, RIA 1027, JCM 4364, BCRC 11467, CGMCC 4.1612, IMET 41583, KCCM 40517, NCIMB 13021, NRRL B-2570, VKM Ac-752, VTT E-072752

straininfo link

  • @ref: 84221
  • straininfo: 38130

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23920229Biocontrol of Rhizoctonia solani damping-off and promotion of tomato plant growth by endophytic actinomycetes isolated from native plants of Algerian Sahara.Goudjal Y, Toumatia O, Yekkour A, Sabaou N, Mathieu F, Zitouni AMicrobiol Res10.1016/j.micres.2013.06.0142013Africa, Northern, Algeria, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Endophytes/classification/genetics/growth & development/*isolation & purification, Lycopersicon esculentum/growth & development/*microbiology, *Microbial Interactions, Molecular Sequence Data, Pest Control, Biological/*methods, Phylogeny, Plant Diseases/*microbiology/prevention & control, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, Rhizoctonia/*growth & development, Sequence Analysis, DNA, Streptomyces/classification/genetics/growth & development/*isolation & purificationEnzymology
Metabolism25703134Optimization of antifungal production by an alkaliphilic and halotolerant actinomycete, Streptomyces sp. SY-BS5, using response surface methodology.Souagui Y, Tritsch D, Grosdemange-Billiard C, Kecha MJ Mycol Med10.1016/j.mycmed.2014.12.0042015Actinobacteria/classification/metabolism, Antifungal Agents/*metabolism, Bioreactors/standards, Calibration, Culture Media/chemistry/pharmacology, Halobacteriaceae/classification/metabolism, Humans, Hydrogen-Ion Concentration, Microbiological Techniques/*standards, *Salt Tolerance, Streptomyces/classification/*metabolism, TemperaturePhylogeny
Biotechnology28179900Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment.Zothanpuia, Passari AK, Chandra P, Leo VV, Mishra VK, Kumar B, Singh BPFront Microbiol10.3389/fmicb.2017.000682017

Reference

@idauthorscataloguedoi/urltitle
9348Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40148)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40148
19410Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40148.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
84221Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38130.1StrainInfo: A central database for resolving microbial strain identifiers
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1