Strain identifier

BacDive ID: 15115

Type strain: Yes

Species: Streptomyces cyaneofuscatus

Strain history: KCC S-0364 <-- IFO 13190 <-- SAJ <-- ISP 5148 <-- INA 99/54.

NCBI tax ID(s): 66883 (species)

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General

@ref: 9348

BacDive-ID: 15115

DSM-Number: 40148

keywords: genome sequence, 16S sequence, Bacteria, psychrophilic

description: Streptomyces cyaneofuscatus DSM 40148 is a psychrophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 66883
  • Matching level: species

strain history

@refhistory
9348<- E.B. Shirling, ISP <- T.P. Preobrazhenskaya, INA
67770KCC S-0364 <-- IFO 13190 <-- SAJ <-- ISP 5148 <-- INA 99/54.

doi: 10.13145/bacdive15115.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces cyaneofuscatus
  • full scientific name: Streptomyces cyaneofuscatus (Kudrina 1957) Pridham et al. 1958 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Streptomyces cavourensis subsp. washingtonensis
    20215Actinomyces cyaneofuscatus

@ref: 9348

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces cyaneofuscatus

full scientific name: Streptomyces cyaneofuscatus (Kudrina 1957) Pridham et al. 1958 emend. Nouioui et al. 2018

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19410Sand yellow10-14 daysISP 2
19410Sand yellow10-14 daysISP 3
19410Sand yellow10-14 daysISP 4
19410Green beige10-14 daysISP 5
19410Sand yellow10-14 daysISP 6
19410Brown beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium namecomplex name
19410noISP 2
19410yesISP 3Aerial Mycelium
19410yesISP 4Aerial Mycelium
19410noISP 5
19410noISP 6
19410noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9348GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19410ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19410ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19410ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19410ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19410ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19410ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
9348positivegrowth20psychrophilic
19410positiveoptimum28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan+energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole+

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19410+++++++++++-+-++++-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19410++--+-+-+++

Isolation, sampling and environmental information

isolation

  • @ref: 9348
  • sample type: soil
  • geographic location: Daghestan
  • country: Russia
  • origin.country: RUS
  • continent: Europe

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_135.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_109;98_120;99_135&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AY999770
  • Sequence Identity:
  • Total samples: 4030
  • soil counts: 2441
  • aquatic counts: 286
  • animal counts: 1009
  • plant counts: 294

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
93481Risk group (German classification)
194101Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
9348Streptomyces cyaneofuscatus gene for 16S rRNA, partial sequence, strain: NBRC 13190AB1848601436ena66883
67770Streptomyces cyaneofuscatus strain JCM 4364 16S ribosomal RNA gene, partial sequenceAY9997701430ena66883

Genome sequences

  • @ref: 67770
  • description: Streptomyces cyaneofuscatus NRRL B-2570
  • accession: GCA_000718135
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 66883

GC content

@refGC-contentmethod
6777071.6genome sequence analysis
6777071fluorimetric

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno94.525no
flagellatedno97.668no
gram-positiveyes91.034no
anaerobicno99.145no
halophileno92.356no
spore-formingyes95.504no
glucose-utilyes89.199no
aerobicyes91.996no
thermophileno98.978yes
glucose-fermentno87.09no

External links

@ref: 9348

culture collection no.: DSM 40148, ATCC 19746, ATCC 23619, CBS 485.68, ETH 24190, IFO 13190, INA 99/54, ISP 5148, NBRC 13190, RIA 1027, JCM 4364, BCRC 11467, CGMCC 4.1612, IMET 41583, KCCM 40517, NCIMB 13021, NRRL B-2570, VKM Ac-752, VTT E-072752

straininfo link

  • @ref: 84221
  • straininfo: 38130

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23920229Biocontrol of Rhizoctonia solani damping-off and promotion of tomato plant growth by endophytic actinomycetes isolated from native plants of Algerian Sahara.Goudjal Y, Toumatia O, Yekkour A, Sabaou N, Mathieu F, Zitouni AMicrobiol Res10.1016/j.micres.2013.06.0142013Africa, Northern, Algeria, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Endophytes/classification/genetics/growth & development/*isolation & purification, Lycopersicon esculentum/growth & development/*microbiology, *Microbial Interactions, Molecular Sequence Data, Pest Control, Biological/*methods, Phylogeny, Plant Diseases/*microbiology/prevention & control, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, Rhizoctonia/*growth & development, Sequence Analysis, DNA, Streptomyces/classification/genetics/growth & development/*isolation & purificationEnzymology
Metabolism25703134Optimization of antifungal production by an alkaliphilic and halotolerant actinomycete, Streptomyces sp. SY-BS5, using response surface methodology.Souagui Y, Tritsch D, Grosdemange-Billiard C, Kecha MJ Mycol Med10.1016/j.mycmed.2014.12.0042015Actinobacteria/classification/metabolism, Antifungal Agents/*metabolism, Bioreactors/standards, Calibration, Culture Media/chemistry/pharmacology, Halobacteriaceae/classification/metabolism, Humans, Hydrogen-Ion Concentration, Microbiological Techniques/*standards, *Salt Tolerance, Streptomyces/classification/*metabolism, TemperaturePhylogeny
Biotechnology28179900Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment.Zothanpuia, Passari AK, Chandra P, Leo VV, Mishra VK, Kumar B, Singh BPFront Microbiol10.3389/fmicb.2017.000682017

Reference

@idauthorscataloguedoi/urltitle
9348Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40148)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40148
19410Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40148.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
84221Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38130.1StrainInfo: A central database for resolving microbial strain identifiers