Strain identifier

BacDive ID: 15100

Type strain: Yes

Species: Streptomyces clavifer

Culture col. no.: DSM 40843, CBS 101.27, NRRL B-2557

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General

@ref: 9843

BacDive-ID: 15100

DSM-Number: 40843

keywords: Bacteria, 16S sequence, mesophilic

description: Streptomyces clavifer DSM 40843 is a mesophilic bacterium that was isolated from limed plot and scab on Solanum tuberosum.

strain history: <- NRRL

doi: 10.13145/bacdive15100.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Streptomycetales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces clavifer
  • full scientific name: Streptomyces clavifer (Millard and Burr 1926) Waksman 1953 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Actinomyces clavifer

@ref: 9843

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces clavifer

full scientific name: Streptomyces clavifer (Millard and Burr 1926) Waksman 1953

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19492Sand yellow10-14 daysISP 2
19492Grey yellow10-14 daysISP 3
1949210-14 daysISP 4
1949210-14 daysISP 5
1949210-14 daysISP 6
1949210-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19492noISP 2
19492noISP 3
19492noISP 4
19492noISP 5
19492noISP 6
19492noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9843GPHF-MEDIUM (DSMZ Medium 553)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium553.pdf
9843STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf
19492ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19492ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19492ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19492ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19492ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19492ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19492positiveoptimum28mesophilic
9843positivegrowth28mesophilic

Physiology and metabolism

compound production

  • @ref: 19492
  • compound: Melanostatin

halophily

  • @ref: 19492
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19492++++++++-++++-++---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19492+++++-+--++

Isolation, sampling and environmental information

isolation

  • @ref: 9843
  • sample type: limed plot and scab on Solanum tuberosum
  • host species: Solanum tuberosum

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Other#Wound
#Host Body-Site#Plant#Fruit (Seed)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
98431Risk group (German classification)
194921Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces clavifer strain AS 4.1604 16S ribosomal RNA gene, partial sequenceFJ4059041387ena68188
20218Streptomyces clavifer gene for 16S ribosomal RNA, partial sequence, strain: JCM 5059D44428120ena68188
20218Streptomyces clavifer gene for 16S rRNA, partial sequence, strain: NBRC 15398AB1846491477ena68188
20218Streptomyces clavifer strain NRRL B-2557 16S ribosomal RNA gene, partial sequenceDQ0266701499ena68188

External links

@ref: 9843

culture collection no.: DSM 40843, CBS 101.27, NRRL B-2557

straininfo link

@refpassport
20218http://www.straininfo.net/strains/166331
20218http://www.straininfo.net/strains/230812
20218http://www.straininfo.net/strains/166333

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9843Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40843)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40843
19492Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40843.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274