Strain identifier

BacDive ID: 151

Type strain: Yes

Species: Actinomyces israelii

Strain history: DSM 43320 <-- M. A. Gerencser WVU 46 <-- ATCC 12102 <-- A. Howell NIH 277.

NCBI tax ID(s): 1120944 (strain), 1659 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 10954

BacDive-ID: 151

DSM-Number: 43320

keywords: anaerobe, Bacteria, 16S sequence, genome sequence, mesophilic

description: Actinomyces israelii DSM 43320 is an anaerobe, mesophilic bacterium that was isolated from human brain abscess.

NCBI tax id

NCBI tax idMatching level
1659species
1120944strain

strain history

doi: 10.13145/bacdive151.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Actinomyces
  • species: Actinomyces israelii
  • full scientific name: Actinomyces israelii (Kruse 1896) Lachner-Sandoval 1898 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Actinobacterium israeli
    20215Streptothrix israeli

@ref: 10954

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Actinomyces

species: Actinomyces israelii

full scientific name: Actinomyces israelii (Kruse 1896) Lachner-Sandoval 1898 emend. Nouioui et al. 2018

type strain: yes

Morphology

colony morphology

  • @ref: 46802
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40615MEDIUM 39 - for ActinomycesyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
10954PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yesName: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled waterhttps://bacmedia.dsmz.de/medium/104
10954COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://bacmedia.dsmz.de/medium/693

culture temp

@refgrowthtypetemperaturerange
40615positivegrowth37mesophilic
10954positivegrowth37mesophilic
46802positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
10954anaerobe
46802anaerobe

murein

  • @ref: 10954
  • murein short key: A11.52
  • type: A5alpha L-Lys-L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838027897tryptophan-energy source
6838029985L-glutamate-degradation
6838016634raffinose+fermentation
6838029016arginine-hydrolysis
6838016199urea-hydrolysis
6837727897tryptophan-energy source
6837716199urea-hydrolysis
6837718257ornithine-degradation
6837717992sucrose-builds acid from
6837717306maltose+builds acid from
6837715824D-fructose+builds acid from
6837717634D-glucose+builds acid from
6837928087glycogen-fermentation
6837917992sucrose+fermentation
6837917716lactose-fermentation
6837917306maltose-fermentation
6837916899D-mannitol-fermentation
6837965327D-xylose-fermentation
6837916988D-ribose-fermentation
683795291gelatin-hydrolysis
6837916199urea-hydrolysis
683794853esculin-hydrolysis
6837917632nitrate-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6838035581indoleno
6837735581indoleno

metabolite tests

@refChebi-IDmetaboliteindole test
6838035581indole-
6837735581indole-

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377proline-arylamidase+3.4.11.5
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6
68379catalase-1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
46802-+-+--+----------+--

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
46802-+++------+--

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
10954---+-+++--+/-+--+---+-++-+-----
10954--+/-+-+++--++------+-++-+-----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
10954human brain abscess
46802Human brain abscessMN,RochesterUSAUSANorth America
67770Human brain abscess

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Organ#Brain
#Host Body-Site#Other#Abscess
#Infection#Patient

Safety information

risk assessment

  • @ref: 10954
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinomyces israelii strain ATCC 12102 16S ribosomal RNA gene, partial sequenceAF058042280ena1659
20218A.israelii 16S rRNA geneX824501441ena1659
20218Actinomyces israelii gene for 16S ribosomal RNA, partial sequence, strain: JCM 12964AB8491231512ena1659

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinomyces israelii DSM 433201120944.3wgspatric1120944
66792Actinomyces israelii strain NCTC129721659.4completepatric1659
66792Actinomyces israelii DSM 433202565956542draftimg1120944
66792Actinomyces israelii NCTC129722843235134completeimg1659
67770Actinomyces israelii DSM 43320, whole genome shotgun sequencing projectJONS00000000ncbi1120944
67770Actinomyces israelii strain NCTC12972 genome assembly, chromosome: 1LR134357ena1659

GC content

  • @ref: 67770
  • GC-content: 70

External links

@ref: 10954

culture collection no.: DSM 43320, ATCC 12102, JCM 12964, CCUG 18307, IMET 10792, CCUG 2271, NIH 277, WVU 46, CIP 103259, KCTC 15377, NCTC 12972

straininfo link

@refpassport
20218http://www.straininfo.net/strains/126953
20218http://www.straininfo.net/strains/96054
20218http://www.straininfo.net/strains/534331

literature

Pubmed-IDtitleauthorsjournalyearmeshtopicDOItopic2
2489354[Transmission electron microscopic studies of fine structure by fast freeze method on Actinomyces israelii ATCC 12102].Watanabe T, Matui T, Suzuki MShigaku1989Actinomycetales/*ultrastructure, Cell Membrane/ultrastructure, Cell Wall/ultrastructure, Cytoplasm/ultrastructure, Freezing
17584471Production of antagonistic substance by Eikenella corrodens isolated from the oral cavity of human beings with and without periodontal disease.Apolonio AC, Carvalho MA, Ribas RN, Sousa-Gaia LG, Santos KV, Lana MA, Nicoli JR, Farias LMJ Appl Microbiol2007Acute Disease, *Antibiosis, Bacteriocins/*biosynthesis, Bacteriological Techniques, Chronic Disease, Culture Media, Eikenella corrodens/*metabolism/physiology, Gingiva/microbiology, Humans, Microbial Sensitivity Tests/methods, Mouth/*microbiology, Periodontitis/*microbiologyCultivation10.1111/j.1365-2672.2006.03211.xPathogenicity
19769270[Initial study on the discrimination of oral common Actinomycetes with metabonomics method].Li MY, Guo Q, Zhou XD, Xiong P, Jia XM, Xiao XR, Li W, Xiao LYHua Xi Kou Qiang Yi Xue Za Zhi2009Actinobacteria, *Actinomyces, Magnetic Resonance Spectroscopy, *Metabolomics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10954Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43320)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43320
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40615Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15037
46802Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 18307)https://www.ccug.se/strain?id=18307
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68377Automatically annotated from API NH
68379Automatically annotated from API Coryne
68380Automatically annotated from API rID32A