Strain identifier

BacDive ID: 15094

Type strain: Yes

Species: Streptomyces cinereus

Strain Designation: 3855

Strain history: KCC A-0040 <-- IMRU 3855.

NCBI tax ID(s): 1955 (species)

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General

@ref: 10703

BacDive-ID: 15094

DSM-Number: 43033

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Streptomyces cinereus 3855 is a mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1955
  • Matching level: species

strain history

@refhistory
10703<- KCC <- 3855, H. Lechevalier, IMRU
67770KCC A-0040 <-- IMRU 3855.

doi: 10.13145/bacdive15094.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces cinereus
  • full scientific name: Streptomyces cinereus (Cross et al. 1963) Goodfellow et al. 1986

  • synonyms

    • @ref: 20215
    • synonym: Microellobosporia cinerea

@ref: 10703

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces cinereus

full scientific name: Streptomyces cinereus (Cross et al. 1963) Goodfellow et al. 1986

strain designation: 3855

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no94.448
6948099.997negative

colony morphology

@refcolony colorincubation periodmedium used
18739Beige (1001)10-14 daysISP 2
18739Beige (1001)10-14 daysISP 3
18739Beige (1001)10-14 daysISP 4
18739Beige (1001)10-14 daysISP 5
18739Beige (1001)10-14 daysISP 6
18739Beige (1001)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18739yesAerial MyceliumWhite (sparse)ISP 2
18739yesAerial MyceliumISP 3
18739yesAerial MyceliumISP 4
18739yesAerial MyceliumISP 5
18739yesAerial MyceliumISP 6
18739yesAerial MyceliumISP 7

multimedia

  • @ref: 10703
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43033.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10703GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18739ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18739ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18739ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18739ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18739ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18739ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10703STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18739positiveoptimum28mesophilic
10703positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.998

observation

@refobservation
20216Antibiotic effective against gram-positive bacteria and mycobacteria
67770quinones: MK-9(H6), MK-9(H4), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan+energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole+

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18739+---+-+-+++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
10703soilPilta, Princess Margaret, glacis (35° 54' N, 14° 24' E)MaltaMLTEurope35.914.4
67770SoilPiltaMaltaMLTEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
187391German classification
107031Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces cinereus gene for 16S rRNA, partial sequenceAB122712564ena1955
20218Streptomyces cinereus gene for 16S ribosomal RNA, partial sequence, strain: JCM 3040D43974120ena1955
20218Streptomyces cinereus strain KCTC 9066 16S ribosomal RNA gene, partial sequenceAY9998051440ena1955
10703Streptomyces cinereus gene for 16S rRNA, partial sequence, strain: NBRC 12247AB1840721452ena1955

Genome sequences

  • @ref: 66792
  • description: Streptomyces cinereus strain JCM 3040
  • accession: 1955.3
  • assembly level: wgs
  • database: patric
  • NCBI tax ID: 1955

GC content

  • @ref: 67770
  • GC-content: 70

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno87.973no
gram-positiveno97.34no
anaerobicno98.402no
aerobicyes70.218no
halophileno77.02no
spore-formingno95.363no
glucose-utilno78.348no
flagellatedno90.652no
thermophileno99.702yes
glucose-fermentno93.858no

External links

@ref: 10703

culture collection no.: DSM 43033, ATCC 15840, CBS 356.67, CUB 301, IFO 12247, IMET 43557, IMRU 3855, JCM 3040, KCC A-0040, NBRC 12247, NCIB 9586, NRRL B-2909, VKM Ac-812, BCRC 11616, CGMCC 4.1672, IFM 1137, IFM 1237, KCTC 9066, KCTC 9194, NBIMCC 1639, NCIMB 9586, RIA 809

straininfo link

  • @ref: 84200
  • straininfo: 264347

literature

  • topic: Phylogeny
  • Pubmed-ID: 29926238
  • title: Streptomyces xiangluensis sp. nov., a novel actinomycete isolated from soil.
  • authors: Zhao J, Li D, Jiang H, Han L, Jiang S, Guo X, Wang X, Xiang W
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-018-1114-9
  • year: 2018
  • mesh: Bacterial Proteins/genetics, Bacterial Typing Techniques, DNA, Bacterial/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Streptomyces/classification/genetics/*isolation & purification
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10703Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43033)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43033
18739Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43033.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84200Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID264347.1StrainInfo: A central database for resolving microbial strain identifiers