Strain identifier

BacDive ID: 15088

Type strain: Yes

Species: Streptomyces chrestomyceticus

Strain Designation: BE-508

Strain history: KCC S-0735 <-- IFO 13444 <-- SAJ <-- ISP 5545 <-- IMRU 3835 <-- A. Di Marco.

NCBI tax ID(s): 1306181 (strain), 68185 (species)

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General

@ref: 9630

BacDive-ID: 15088

DSM-Number: 40545

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production

description: Streptomyces chrestomyceticus BE-508 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

NCBI tax idMatching level
68185species
1306181strain

strain history

@refhistory
9630<- E.B. Shirling, ISP <- R.E. Gordon, IMRU
67770KCC S-0735 <-- IFO 13444 <-- SAJ <-- ISP 5545 <-- IMRU 3835 <-- A. Di Marco.

doi: 10.13145/bacdive15088.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces chrestomyceticus
  • full scientific name: Streptomyces chrestomyceticus Canevazzi and Scotti 1959 (Approved Lists 1980)

@ref: 9630

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces chrestomyceticus

full scientific name: Streptomyces chrestomyceticus Canevazzi and Scotti 1959

strain designation: BE-508

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no91.404
69480100positive

colony morphology

@refcolony colorincubation periodmedium used
19474Yellow10-14 daysISP 2
1947410-14 daysISP 3
19474Beige10-14 daysISP 4
19474Beige10-14 daysISP 5
1947410-14 daysISP 6
1947410-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19474yesAerial MyceliumWhiteISP 2
19474yesAerial MyceliumWhiteISP 3
19474yesAerial MyceliumBeigeISP 4
19474yesAerial MyceliumBeigeISP 5
19474noISP 6
19474yesAerial MyceliumWhiteISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9630GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19474ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19474ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19474ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19474ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19474ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19474ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9630ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
19474positiveoptimum28mesophilic
9630positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

tolerance

  • @ref: 19474
  • compound: Lysozyme
  • percentage: 1

spore formation

@refspore descriptiontype of sporespore formationconfidence
19474Formation of spore chains (spirales)(Sp), spore surface smoothsporeyes
69481yes100
69480yes100

compound production

@refcompound
9630aminocidin
9630neomycin E
9630neomycin F
9630paramomycin
19474Pyrrolostatin

halophily

  • @ref: 19474
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1947416634raffinose-
1947426546rhamnose-
1947428757fructose+
1947429864mannitol+
1947417268myo-inositol-
1947418222xylose-
1947417992sucrose-
1947422599arabinose-
1947417234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refmetaboliteproductionChebi-ID
67770neomycin Eyes
67770neomycin Fyes
68368acetoinyes15688
68368indoleno35581
68368hydrogen sulfideno16136

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase+4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19474+++++-++-++

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
96301Risk group (German classification)
194741Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces chrestomyceticus strain CGMCC 4.1657 16S ribosomal RNA gene, partial sequenceJN5660251378ena1306181
20218Streptomyces chrestomyceticus 16S rRNA gene, type strain DSM 40545TAJ6216091497ena1306181
20218Streptomyces chrestomyceticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4735D44319121ena1306181
20218Streptomyces chrestomyceticus gene for 16S rRNA, partial sequence, strain: NBRC 13444AB1844071480ena1306181
20218Streptomyces chrestomyceticus JCM 4735 strain NRRL B-3293 16S ribosomal RNA gene, partial sequenceDQ0266331483ena1306181
20218Streptomyces chrestomyceticus JCM 4735 strain NRRL B-3310 16S ribosomal RNA gene, partial sequenceAY9997951457ena1306181

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces chrestomyceticus JCM 4735GCA_001315465contigncbi1306181
66792Streptomyces chrestomyceticus JCM 47351306181.9wgspatric1306181
66792Streptomyces chrestomyceticus JCM 4735 strain NBRC 134441306181.8wgspatric1306181
66792Streptomyces chrestomyceticus JCM 47352734481989draftimg1306181
67770Streptomyces chrestomyceticus JCM 4735 NBRC 13444GCA_003865135contigncbi1306181

GC content

  • @ref: 67770
  • GC-content: 72
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno90.663no
gram-positiveyes89.441no
anaerobicno94.706no
halophileno92.5no
spore-formingyes58.907yes
thermophileno99.016yes
glucose-utilyes87.301yes
aerobicyes94.632no
flagellatedno97.624no
glucose-fermentno85.348no

External links

@ref: 9630

culture collection no.: DSM 40545, ATCC 14947, CBS 745.72, DSM 40820, IFO 13444, IMRU 3835, ISP 5545, NBRC 13444, NCIB 8995, RIA 1405, JCM 4735, KCC S-0735, BCRC 12173, CGMCC 4.1657, MTCC 6922, NCAIM B.01478, NCIMB 10999, NCIMB 8995, NRRL B-3293, NRRL B-3310, NRRL B-3672

straininfo link

  • @ref: 84194
  • straininfo: 36211

literature

  • topic: Phylogeny
  • Pubmed-ID: 23645019
  • title: Streptomyces kebangsaanensis sp. nov., an endophytic actinomycete isolated from an ethnomedicinal plant, which produces phenazine-1-carboxylic acid.
  • authors: Sarmin NIM, Tan GYA, Franco CMM, Edrada-Ebel R, Latip J, Zin NM
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.047878-0
  • year: 2013
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Malaysia, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phenazines/metabolism, *Phylogeny, Plants, Medicinal/*microbiology, Portulaca/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/analysis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9630Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40545)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40545
19474Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40545.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84194Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36211.1StrainInfo: A central database for resolving microbial strain identifiers