Strain identifier

BacDive ID: 15053

Type strain: Yes

Species: Streptomyces bluensis

Strain history: KCC S-0729 <-- IFO 13460 <-- SAJ <-- ISP 5564 <-- NRRL 2876 <-- Upjohn Co., USA; UC 2478.

NCBI tax ID(s): 33897 (species)

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General

@ref: 9646

BacDive-ID: 15053

DSM-Number: 40564

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production

description: Streptomyces bluensis DSM 40564 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 33897
  • Matching level: species

strain history

@refhistory
9646<- E.B. Shirling, ISP <- NRRL <- A. Dietz, Upjohn, U-12898
67770KCC S-0729 <-- IFO 13460 <-- SAJ <-- ISP 5564 <-- NRRL 2876 <-- Upjohn Co., USA; UC 2478.

doi: 10.13145/bacdive15053.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces bluensis
  • full scientific name: Streptomyces bluensis Mason et al. 1963 (Approved Lists 1980)

@ref: 9646

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces bluensis

full scientific name: Streptomyces bluensis Mason et al. 1963

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no92.594
69480100positive

colony morphology

@refcolony colorincubation periodmedium used
19476Beige10-14 daysISP 2
1947610-14 daysISP 3
1947610-14 daysISP 4
19476Beige10-14 daysISP 5
19476Beige10-14 daysISP 6
19476Beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19476yesAerial MyceliumPastel blue (5024)ISP 2
19476yesAerial MyceliumPastel blue (5024)ISP 3
19476yesAerial MyceliumPastel blue (5024)ISP 4
19476noISP 5
19476noISP 6
19476noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9646GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19476ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19476ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19476ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19476ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19476ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19476ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9646ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
19476positiveoptimum28mesophilic
9646positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes100
69480yes100

compound production

@refcompound
9646glebomycin
20216glebomycin (bluensomycin
19476Bluensomycin

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1947662968cellulose-
1947616634raffinose-
1947626546rhamnose-
1947628757fructose-
1947629864mannitol-
1947617268myo-inositol+
1947618222xylose+
1947617992sucrose+
1947622599arabinose-
1947617234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refmetaboliteproductionChebi-ID
67770bluensomycinyes
68368acetoinyes15688
68368indoleno35581
68368hydrogen sulfideno16136

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19476+++-+++++++++-++++-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19476+++-+-+--++

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
96461Risk group (German classification)
194761Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces bluensis 16S rRNA gene, strain ISP5564X793241520ena33897
20218Streptomyces bluensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 4729D44316121ena33897
20218Streptomyces bluensis gene for 16S rRNA, partial sequence, strain: NBRC 13460AB1844211477ena33897

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces bluensis JCM 4729GCA_014650715contigncbi33897
66792Streptomyces bluensis strain JCM 472933897.3wgspatric33897

GC content

  • @ref: 67770
  • GC-content: 70.2
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes88.246no
anaerobicno99.3no
halophileno91.852no
spore-formingyes95.496no
glucose-utilyes90.551yes
flagellatedno97.942no
thermophileno96.813yes
aerobicyes93.936no
motileno95.082no
glucose-fermentno89.626no

External links

@ref: 9646

culture collection no.: DSM 40564, ATCC 27420, CBS 761.72, IFO 13460, ISP 5564, NBRC 13460, NRRL 2876, RIA 1421, UC 12898, JCM 4729, BCRC 15180, CBS 239.69, CGMCC 4.1463, LMG 5969, NCIMB 9754

straininfo link

  • @ref: 84159
  • straininfo: 5010

literature

  • topic: Metabolism
  • Pubmed-ID: 12620633
  • title: Isolation and characterization of bluensomycin biosynthetic genes from Streptomyces bluensis.
  • authors: Jung YG, Kang SH, Hyun CG, Yang YY, Kang CM, Suh JW
  • journal: FEMS Microbiol Lett
  • DOI: 10.1016/S0378-1097(03)00019-3
  • year: 2003
  • mesh: Carbohydrate Sequence, Cloning, Molecular, Dihydrostreptomycin Sulfate/*analogs & derivatives/immunology/*metabolism, *Genes, Bacterial, Glucose/*analogs & derivatives/metabolism, Glucosephosphates/metabolism, Mannose-6-Phosphate Isomerase/analysis, Multigene Family, Mutagenesis, Insertional, Nucleotidyltransferases/analysis, Open Reading Frames, Streptomyces/classification/*genetics/metabolism, Thymine Nucleotides/metabolism
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9646Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40564)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40564
19476Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40564.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84159Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID5010.1StrainInfo: A central database for resolving microbial strain identifiers