Strain identifier

BacDive ID: 15039

Type strain: No

Species: Kitasatospora aureofaciens

Strain Designation: 2758 FI

Strain history: KCC S-0321 <-- IPV 1830 <-- E. Baldacci 2758 FI.

NCBI tax ID(s): 1894 (species)

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General

@ref: 9637

BacDive-ID: 15039

DSM-Number: 40554

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, antibiotic compound production

description: Kitasatospora aureofaciens 2758 FI is a mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 1894
  • Matching level: species

strain history

@refhistory
9637<- E.B. Shirling, ISP <- E. Baldacci, 2758 FI
67770KCC S-0321 <-- IPV 1830 <-- E. Baldacci 2758 FI.

doi: 10.13145/bacdive15039.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Kitasatospora
  • species: Kitasatospora aureofaciens
  • full scientific name: Kitasatospora aureofaciens (Duggar 1948) Labeda et al. 2017

  • synonyms

    @refsynonym
    20215Streptomyces avellaneus
    20215Streptomyces aureofaciens
    20215Streptomyces psammoticus
    20215Kitasatospora psammotica

@ref: 9637

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Kitasatospora

species: Kitasatospora aureofaciens

full scientific name: Kitasatospora aureofaciens (Duggar 1948) Labeda et al. 2017

strain designation: 2758 FI

type strain: no

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19475Beige brown10-14 daysISP 2
19475Beige brown10-14 daysISP 3
19475Beige brown10-14 daysISP 4
19475Beige brown10-14 daysISP 5
19475Beige brown10-14 daysISP 6
19475Beige brown10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
19475yesAerial MyceliumISP 2
19475yesAerial MyceliumISP 3Light grey (7035)
19475noISP 4
19475noISP 5
19475noISP 6
19475noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9637GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19475ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19475ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19475ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19475ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19475ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19475ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9637ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
19475positiveoptimum28mesophilic
9637positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

compound production

@refcompound
9637tetracycline
19475Deacetylchromomycin

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1947562968cellulose+
1947516634raffinose-
1947526546rhamnose+
1947528757fructose+
1947529864mannitol-
1947517268myo-inositol-
1947518222xylose-
1947517992sucrose+
1947522599arabinose-
1947517234glucose+
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6777027902tetracyclineyes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19475+++++++--++-+-+--+-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19475++++-----+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
9637soilItalyITAEurope
67770Soil in the caverns of Caudano at CuneoItalyITAEuropePiedmont

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_224.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_148;97_159;98_191;99_224&stattab=map
  • Last taxonomy: Streptomycetaceae
  • 16S sequence: AY999903
  • Sequence Identity:
  • Total samples: 4218
  • soil counts: 3325
  • aquatic counts: 173
  • animal counts: 599
  • plant counts: 121

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
96371Risk group (German classification)
194751Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces avellaneus gene for 16S rRNA, partial sequenceAB122722564ena68178
20218Streptomyces avellaneus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4725D44312120ena68178
20218Streptomyces avellaneus gene for 16S rRNA, partial sequence, strain: NBRC 13451AB1844131466ena68178
20218Streptomyces avellaneus strain NRRL B-3447 16S ribosomal RNA gene, partial sequenceAY9999031328ena68178

Genome sequences

  • @ref: 67770
  • description: Kitasatospora aureofaciens NRRL B-3447
  • accession: GCA_000721255
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1894

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno94.753no
flagellatedno98.234no
gram-positiveyes93.745no
anaerobicno99.331no
aerobicyes93.431no
halophileno94.704no
spore-formingyes91.685no
glucose-utilyes90.831yes
thermophileno96.036yes
glucose-fermentno89.929no

External links

@ref: 9637

culture collection no.: DSM 40554, ATCC 23730, CBS 752.72, IFO 13451, IMRU 3911, IPV 1830, ISP 5554, JCM 4321, NBRC 13451, NRRL B-3447, RIA 1412, BCRC 12219, CGMCC 4.1687, IMI 126840, JCM 4725, NCIMB 11000, VKM Ac-1720

straininfo link

  • @ref: 84145
  • straininfo: 92722

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9637Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40554)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40554
19475Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40554.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
84145Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92722.1StrainInfo: A central database for resolving microbial strain identifiers