Strain identifier

BacDive ID: 15015

Type strain: Yes

Species: Kitasatospora aureofaciens

Strain Designation: A-377

Culture col. no.: DSM 40127, ATCC 10762, ATCC 23884, CBS 664.68, IFO 12594, IFO 12843, ISP 5127, JCM 4008, KACC 20180, NBRC 12594, NBRC 12843, NRRL 2209, RIA 1129

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 5.0 (current version):
version 4.1:
version 4.0:
version 3.0:
version 2.1:
version 2.0:
version 1.0:
version 5.0 (current version)

General

@ref: 9330

BacDive-ID: 15015

DSM-Number: 40127

keywords: mesophilic, spore-forming, Bacteria, 16S sequence, genome sequence, antibiotic compound production

description: Kitasatospora aureofaciens A-377 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

strain history: <- E.B. Shirling, ISP <- E. Backus, Lederle Labs., A-377

doi: 10.13145/bacdive15015.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Streptomycetales
  • family: Streptomycetaceae
  • genus: Kitasatospora
  • species: Kitasatospora aureofaciens
  • full scientific name: Kitasatospora aureofaciens (Duggar 1948) Labeda et al. 2017
  • synonyms

    @refsynonym
    20215Streptomyces avellaneus
    20215Streptomyces aureofaciens

@ref: 9330

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Kitasatospora

species: Kitasatospora aureofaciens

full scientific name: Kitasatospora aureofaciens (Duggar 1948) Labeda et al. 2017

strain designation: A-377

type strain: yes

Morphology

colony morphology

@refincubation periodmedium usedcolony color
1940510-14 daysISP 2
1940510-14 daysISP 3
1940510-14 daysISP 4
1940510-14 daysISP 5
1940510-14 daysISP 6Yellow
1940510-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19405yesAerial MyceliumGreyISP 2
19405yesAerial MyceliumGreyISP 3
19405yesAerial MyceliumGreyISP 4
19405yesAerial MyceliumGreyISP 5
19405yesAerial MyceliumWhiteISP 6
19405yesAerial MyceliumWhiteISP 7

multimedia

  • @ref: 9330
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40127.jpg
  • caption: Medium 84 28°C
  • license or copyright: (C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9330ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf
9330GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
19405ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19405ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19405ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19405ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19405ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19405ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
37599MEDIUM 122 - for StreptomycesyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (2.000 g);Starch maize (10.000 g)

culture temp

@refgrowthtypetemperaturerange
19405positiveoptimum28mesophilic
9330positivegrowth28mesophilic
37599positivegrowth30mesophilic

Physiology and metabolism

spore formation

  • @ref: 19405
  • spore description: Formation of spore chains: rectiflixibilis,spore surface: smooth
  • spore formation: yes

compound production

@refcompound
9330chlortetracycline
9330tetracycline
9330aureomycin

metabolite utilization

@refChebi-IDmetaboliteutilization activity
1940562968cellulose-
1940516634raffinose-
1940526546rhamnose-
1940528757fructose+
1940529864mannitol+
1940517268myo-inositol+
1940518222xylose+/-
1940517992sucrose+
1940522599arabinose+
1940517234glucose+

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19405++++--+---+

Isolation, sampling and environmental information

isolation

  • @ref: 9330
  • sample type: Soil, timothy field
  • geographic location: Missouri
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
93301Risk group (German classification)
194051Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces aureofaciens strain DSM 40127 clone ITS 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296975238ena1894
20218Streptomyces aureofaciens strain DSM 40127 clone ITS18 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296976238ena1894
20218Streptomyces aureofaciens strain DSM 40127 clone ITS19 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296977236ena1894
20218Streptomyces aureofaciens strain DSM 40127 clone ITS20 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296978239ena1894
20218Streptomyces aureofaciens strain DSM 40127 clone ITS30 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296979236ena1894
20218Streptomyces aureofaciens strain DSM 40127 clone ITS9 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296980241ena1894
20218Streptomyces aureofaciens strain IMET 43577 16S ribosomal RNA gene, partial sequenceAY2891161509ena1894
20218Streptomyces bobili gene for 16S rRNAAB0458761483ena67280
20218Streptomyces aureofaciens gene for 16S rRNAAB0458811473ena1894
20218Streptomyces aureofaciens gene for 16S ribosomal RNA, partial sequence, strain: JCM 4624D44273120ena1894
20218Streptomyces aureofaciens gene for 16S rRNA, partial sequence, strain: NBRC 12594AB1841051468ena1894
20218Streptomyces aureofaciens gene for 16S rRNA, partial sequence, strain: NBRC 12843AB1841891460ena1894
20218Streptomyces aureofaciens gene for 16S rRNA, partial sequence, strain: NBRC 3712AB1847831468ena1894
20218Streptomyces aureofaciens 16S rRNA gene, strain NRRL 2209Y155041469ena1894
20218Shewanella sp. SPSA4 partial 16S rRNA gene, strain SPSA4AM884366486ena470054
20218Pseudomonas aeruginosa strain RPATPSA4 16S ribosomal RNA gene, partial sequenceJQ303330650ena287
9330Streptomyces aureofaciens 16S ribosomal RNA gene, partial sequenceAY2076081512ena1894

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kitasatospora aureofaciens ATCC 10762GCA_001188955contigncbi1894
66792Kitasatospora aureofaciens NRRL 2209GCA_000978515contigncbi1894
66792Streptomyces aureofaciens strain ATCC 107621894.10wgspatric1894
66792Streptomyces aureofaciens strain NRRL 22091894.9wgspatric1894

External links

@ref: 9330

culture collection no.: DSM 40127, ATCC 10762, ATCC 23884, CBS 664.68, IFO 12594, IFO 12843, ISP 5127, JCM 4008, KACC 20180, NBRC 12594, NBRC 12843, NRRL 2209, RIA 1129

straininfo link

@refpassport
20218http://www.straininfo.net/strains/53845
20218http://www.straininfo.net/strains/53839
20218http://www.straininfo.net/strains/228008
20218http://www.straininfo.net/strains/53854
20218http://www.straininfo.net/strains/53855
20218http://www.straininfo.net/strains/53859
20218http://www.straininfo.net/strains/53860
20218http://www.straininfo.net/strains/333978
20218http://www.straininfo.net/strains/333979
20218http://www.straininfo.net/strains/95450
20218http://www.straininfo.net/strains/53870

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9330Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40127)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40127
19405Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40127.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37599Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/9594
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)