Strain identifier
BacDive ID: 14992
Type strain: 
Species: Streptomyces anulatus
Strain Designation: Beijerinck
Strain history: KCC S-0721 <-- IFO 13369 <-- SAJ <-- ISP 5361 <-- CBS Beijerinck strain.
NCBI tax ID(s): 1892 (species)
version 8.1 (current version)
General
@ref: 9282
BacDive-ID: 14992
DSM-Number: 40361
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive
description: Streptomyces anulatus Beijerinck is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium.
NCBI tax id
- NCBI tax id: 1892
- Matching level: species
strain history
| @ref | history |
|---|---|
| 9282 | <- E.B. Shirling, ISP <- CBS, "Beijerinck" |
| 67770 | KCC S-0721 <-- IFO 13369 <-- SAJ <-- ISP 5361 <-- CBS Beijerinck strain. |
doi: 10.13145/bacdive14992.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces anulatus
- full scientific name: Streptomyces anulatus (Beijerinck 1912) Waksman 1953 (Approved Lists 1980)
synonyms
@ref synonym 20215 Streptomyces fluorescens 20215 Streptomyces chrysomallus 20215 Actinomyces fluorescens 20215 Streptomyces citreofluorescens 20215 Actinomyces praecox 20215 Actinomyces citreofluorescens 20215 Streptomyces praecox
@ref: 9282
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces anulatus
full scientific name: Streptomyces anulatus (Beijerinck 1912) Waksman 1953 emend. Lanoot et al. 2005
strain designation: Beijerinck
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 100
colony morphology
| @ref | incubation period | medium used |
|---|---|---|
| 19444 | 10-14 days | ISP 2 |
| 19444 | 10-14 days | ISP 3 |
| 19444 | 10-14 days | ISP 4 |
| 19444 | 10-14 days | ISP 5 |
multicellular morphology
| @ref | forms multicellular complex | complex name | complex color | medium name |
|---|---|---|---|---|
| 19444 | yes | Aerial Mycelium | White | ISP 2 |
| 19444 | yes | Aerial Mycelium | White | ISP 3 |
| 19444 | yes | Aerial Mycelium | White | ISP 4 |
| 19444 | yes | Aerial Mycelium | White | ISP 5 |
multimedia
- @ref: 9282
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40361.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 9282 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 19444 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19444 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19444 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19444 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 9282 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 9282 | positive | growth | 28 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
| @ref | spore description | spore formation | confidence |
|---|---|---|---|
| 19444 | Formation of spore chains: rectiflixibilis, spore surface: smooth | yes | |
| 69481 | yes | 100 | |
| 69480 | yes | 100 |
Isolation, sampling and environmental information
taxonmaps
- @ref: 69479
- File name: preview.99_135.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_109;98_120;99_135&stattab=map
- Last taxonomy: Streptomyces
- 16S sequence: DQ026637
- Sequence Identity:
- Total samples: 4030
- soil counts: 2441
- aquatic counts: 286
- animal counts: 1009
- plant counts: 294
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 9282 | 1 | Risk group (German classification) |
| 19444 | 1 | Risk group (German classification) |
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Streptomyces anulatus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4721 | D44309 | 120 | ena | 1892 |
| 20218 | Streptomyces anulatus gene for 16S rRNA, partial sequence, strain: NBRC 13369 | AB184875 | 1475 | ena | 1892 |
| 20218 | Streptomyces anulatus strain NRRL B-2000 16S ribosomal RNA gene, partial sequence | DQ026637 | 1493 | ena | 1892 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Streptomyces anulatus JCM 4721 | GCA_014650675 | scaffold | ncbi | 1892 |
| 66792 | Streptomyces anulatus strain JCM 4721 | 1892.22 | wgs | patric | 1892 |
GC content
- @ref: 67770
- GC-content: 71
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| flagellated | no | 97.873 | no |
| gram-positive | yes | 88.258 | no |
| anaerobic | no | 99.199 | no |
| aerobic | yes | 94.71 | no |
| halophile | no | 93.713 | no |
| spore-forming | yes | 92.692 | yes |
| thermophile | no | 99.194 | yes |
| glucose-util | yes | 88.762 | no |
| motile | no | 94.307 | no |
| glucose-ferment | no | 86.434 | no |
External links
@ref: 9282
culture collection no.: DSM 40361, ATCC 27416, CBS 100.18, CBS 670.72, IFO 13369, ISP 5361, JCM 4721, KCC S-0721, NBRC 13369, RIA 1330, BCRC 15152, CGMCC 4.1421, IMET 43334, KCTC 9756, LMG 19301, LMG 8583, NRRL B-2000, VKM Ac-728, VTT E-991427
straininfo link
- @ref: 84100
- straininfo: 13326
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Enzymology | 18307344 | TMG-chitotriomycin, an enzyme inhibitor specific for insect and fungal beta-N-acetylglucosaminidases, produced by actinomycete Streptomyces anulatus NBRC 13369. | Usuki H, Nitoda T, Ichikawa M, Yamaji N, Iwashita T, Komura H, Kanzaki H | J Am Chem Soc | 10.1021/ja077641f | 2008 | Animals, Bacillus/classification/enzymology, Carbohydrate Conformation, Carbohydrate Sequence, Chitinases/antagonists & inhibitors, Enzyme Activation/drug effects, Enzyme Inhibitors/chemistry/*metabolism/*pharmacology, Insecta, Molecular Sequence Data, Penicillium/*enzymology, Sensitivity and Specificity, Species Specificity, Spodoptera/*enzymology, Streptomyces/classification/*metabolism, Streptomyces griseus/enzymology, Structure-Activity Relationship, Sugar Alcohols/chemistry/*pharmacology, beta-N-Acetyl-Galactosaminidase/*antagonists & inhibitors | Phylogeny |
| Enzymology | 21373681 | MS/MS fragmentation-guided search of TMG-chitooligomycins and their structure-activity relationship in specific beta-N-acetylglucosaminidase inhibition. | Usuki H, Yamamoto Y, Kumagai Y, Nitoda T, Kanzaki H, Hatanaka T | Org Biomol Chem | 10.1039/c0ob01090a | 2011 | Acetylglucosaminidase/*antagonists & inhibitors/chemistry, Aspergillus oryzae/drug effects/enzymology, Enzyme Inhibitors/*chemistry/pharmacology, Glucosamine/*analogs & derivatives/chemistry, Models, Molecular, Molecular Structure, Oligomycins/*chemistry, Protein Structure, Tertiary, Spectrometry, Mass, Electrospray Ionization, Streptomyces coelicolor/drug effects/enzymology, Structure-Activity Relationship, Tandem Mass Spectrometry | Pathogenicity |
| Phylogeny | 34889725 | Streptomyces silvae sp. nov., isolated from forest soil. | Besaury L, Martinet L, Muhle E, Clermont D, Remond C | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005147 | 2021 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Forests, France, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, *Streptomyces/classification/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
| 35222341 | Exploration of Diverse Secondary Metabolites From Streptomyces sp. YINM00001, Using Genome Mining and One Strain Many Compounds Approach. | Liu T, Ren Z, Chunyu WX, Li GD, Chen X, Zhang ZT, Sun HB, Wang M, Xie TP, Wang M, Chen JY, Zhou H, Ding ZT, Yin M | Front Microbiol | 10.3389/fmicb.2022.831174 | 2022 | |||
| Phylogeny | 36048630 | Streptomyces durocortorensis sp. nov., isolated from oak rhizosphere. | Cassarini M, Remond C, Muhle E, Clermont D, Besaury L | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005480 | 2022 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids, Phylogeny, *Quercus, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, Soil Microbiology, *Streptomyces | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 9282 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40361) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40361 | |||
| 19444 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40361.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 84100 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID13326.1 | StrainInfo: A central database for resolving microbial strain identifiers |