Strain identifier

BacDive ID: 14863

Type strain: Yes

Species: Kitasatospora paranensis

Strain Designation: 2292-041

Strain history: I. Groth 2292-041 (=HKI 0190) <-- Ch. Weigel.

NCBI tax ID(s): 258053 (species)

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General

@ref: 11975

BacDive-ID: 14863

DSM-Number: 44788

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Kitasatospora paranensis 2292-041 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from a rhizophere soil sample of Maytenus ilicifolia.

NCBI tax id

  • NCBI tax id: 258053
  • Matching level: species

strain history

@refhistory
11975<- I. Groth, HKI, 07745 Jena, Germany; 2292-041 <- Ch. Weigel
67770I. Groth 2292-041 (=HKI 0190) <-- Ch. Weigel.

doi: 10.13145/bacdive14863.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Kitasatospora
  • species: Kitasatospora paranensis
  • full scientific name: Kitasatospora paranensis Groth et al. 2004

@ref: 11975

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Kitasatospora

species: Kitasatospora paranensis

full scientific name: Kitasatospora paranensis Groth et al. 2004

strain designation: 2292-041

type strain: yes

Morphology

cell morphology

  • @ref: 31218
  • gram stain: positive
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
20118Beige (1001)10-14 daysISP 2
20118Beige (1001)10-14 daysISP 3
20118Beige (1001)10-14 daysISP 4
20118Beige (1001)10-14 daysISP 5
20118Beige (1001)10-14 daysISP 6
20118Beige (1001)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
20118yesAerial MyceliumDusty greyISP 2
20118yesAerial MyceliumDusty greyISP 3
20118yesAerial MyceliumDusty greyISP 4
20118noISP 5
20118noISP 6
20118yesAerial MyceliumDusty greyISP 7

pigmentation

  • @ref: 31218
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11975ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
20118ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20118ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20118ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20118ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20118ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20118ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11975GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
11975STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
11975positivegrowth28mesophilic
20118positiveoptimum28mesophilic
31218positivegrowth10-37
31218positiveoptimum26.5mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
31218positivegrowth05-09alkaliphile
31218positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 31218
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31218
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
31218NaClpositivegrowth0-3.5 %
31218NaClpositiveoptimum1.75 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-9(H4), MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2011817234glucose+
2011822599arabinose+
2011817992sucrose-
2011818222xylose-
2011817268myo-inositol-
2011829864mannitol-
2011828757fructose+
2011826546rhamnose-
2011816634raffinose-
2011862968cellulose+
3121822599arabinose+carbon source
3121817234glucose+carbon source
3121826546rhamnose+carbon source
3121818222xylose+carbon source
3121817632nitrate+reduction

metabolite production

  • @ref: 31218
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
31218acid phosphatase+3.1.3.2
31218alkaline phosphatase+3.1.3.1
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20118++++++---++-+-++---

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
11975a rhizophere soil sample of Maytenus ilicifoliaMaytenus ilicifoliaContenda, state ParanaBrazilBRAMiddle and South America
67770Rhizosphere soil of Maytenus ilicifoliaMaytenus ilicifoliaContenda, Parana StateBrazilBRAMiddle and South America

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants#Tree
#Host Body-Site#Plant#Rhizosphere

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
119751Risk group (German classification)
201181German classification

Sequence information

16S sequences

  • @ref: 11975
  • description: Kitasatospora paranensis strain HKI 0190 16S ribosomal RNA gene, partial sequence
  • accession: AY442268
  • length: 1446
  • database: ena
  • NCBI tax ID: 258053

External links

@ref: 11975

culture collection no.: DSM 44788, HKI 0190, NCIMB 13976, JCM 13005, IAM 15270, NBRC 101837

straininfo link

  • @ref: 83976
  • straininfo: 134434

literature

  • topic: Phylogeny
  • Pubmed-ID: 15545445
  • title: Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp. nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp. nov. and K. terrestris sp. nov.
  • authors: Groth I, Rodriguez C, Schutze B, Schmitz P, Leistner E, Goodfellow M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63070-0
  • year: 2004
  • mesh: Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis/isolation & purification, Genes, rRNA/genetics, Hydrogen-Ion Concentration, Japan, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Saline Solution, Hypertonic, Sequence Analysis, DNA, *Soil Microbiology, Streptomycetaceae/*classification/cytology/*isolation & purification/physiology, Temperature
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
11975Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44788)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44788
20118Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44788.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31218Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2754428776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
83976Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID134434.1StrainInfo: A central database for resolving microbial strain identifiers