Strain identifier

BacDive ID: 14857

Type strain: Yes

Species: Kitasatospora putterlickiae

Strain Designation: F 18-98

Strain history: CIP <- 2003, NCIMB

NCBI tax ID(s): 221725 (species)

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General

@ref: 11861

BacDive-ID: 14857

DSM-Number: 44665

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Kitasatospora putterlickiae F 18-98 is an obligate aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from soil .

NCBI tax id

  • NCBI tax id: 221725
  • Matching level: species

strain history

@refhistory
11861<- I. Groth, HKI, 07745 Jena, Germany; F 18-98 <- Univ. Bonn <- C. Pullen
367052003, NCIMB
67770I. Groth F18-98 (=Uni Bonn 18) <-- Inst. Pharm. Biol., Univ. Bonn.
122078CIP <- 2003, NCIMB

doi: 10.13145/bacdive14857.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Kitasatospora
  • species: Kitasatospora putterlickiae
  • full scientific name: Kitasatospora putterlickiae Groth et al. 2003

@ref: 11861

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Kitasatospora

species: Kitasatospora putterlickiae

full scientific name: Kitasatospora putterlickiae Groth et al. 2003

strain designation: F 18-98

type strain: yes

Morphology

cell morphology

  • @ref: 122078
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

@refcolony colorincubation periodmedium used
19687Brown beige10-14 daysISP 2
1968710-14 daysISP 3
1968710-14 daysISP 4
19687Beige10-14 daysISP 5
19687Beige10-14 daysISP 6
19687Brown beige10-14 daysISP 7
122078

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19687yesAerial MyceliumDusty greyISP 2
19687yesAerial MyceliumDusty greyISP 3
19687yesAerial MyceliumDusty greyISP 4
19687noISP 5
19687noISP 6
19687noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11861GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19687ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19687ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19687ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19687ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19687ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19687ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
36705MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
11861ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
11861TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
122078CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
11861positivegrowth28mesophilic
19687positiveoptimum28mesophilic
36705positivegrowth30mesophilic
67770positivegrowth28mesophilic
122078positivegrowth25-37mesophilic
122078nogrowth10psychrophilic
122078nogrowth41thermophilic
122078nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122078
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
122078NaClpositivegrowth0-2 %
122078NaClnogrowth4 %
122078NaClnogrowth6 %
122078NaClnogrowth8 %
122078NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-9(H4), MK-9(H2), MK-10(H4), MK-11(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1968717234glucose+
1968722599arabinose-
1968717992sucrose-
1968718222xylose-
1968717268myo-inositol-
1968729864mannitol-
1968728757fructose-
1968726546rhamnose-
1968716634raffinose-
1968762968cellulose+
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12207816947citrate-carbon source
1220784853esculin-hydrolysis
12207817632nitrate-reduction
12207816301nitrite-reduction
12207817632nitrate-respiration

antibiotic resistance

  • @ref: 122078
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 122078
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12207815688acetoin-
12207817234glucose-

enzymes

@refvalueactivityec
122078oxidase-
122078beta-galactosidase+3.2.1.23
122078alcohol dehydrogenase+1.1.1.1
122078gelatinase+/-
122078amylase-
122078DNase-
122078caseinase+3.4.21.50
122078catalase+1.11.1.6
122078tween esterase-
122078lecithinase+
122078lipase-
122078lysine decarboxylase+4.1.1.18
122078ornithine decarboxylase+4.1.1.17
122078phenylalanine ammonia-lyase-4.3.1.24
122078tryptophan deaminase-
122078urease-3.5.1.5
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19687+++-+++-+++-+-++---
122078-++-+-+--+--+-++----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122078----+/-----------------+/---+/-------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122078+--+-----++-------++-----------+-----------------+---------+++++--------++++---++-----+-++++-+--++-

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinent
11861soil (rhizosphere of Putterlickia verrucosa)Putterlickia verrucosaSouth AfricaZAFAfrica
67770Rhizosphere of Putterlickia verrucosaPutterlickia verrucosaSouth AfricaZAFAfrica

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants#Tree
#Host Body-Site#Plant#Rhizosphere

taxonmaps

  • @ref: 69479
  • File name: preview.99_4140.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_148;97_159;98_177;99_4140&stattab=map
  • Last taxonomy: Streptomycetaceae
  • 16S sequence: AY189976
  • Sequence Identity:
  • Total samples: 153
  • soil counts: 144
  • aquatic counts: 1
  • animal counts: 6
  • plant counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
118611Risk group (German classification)
196871Risk group (German classification)
1220781Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Kitasatospora putterlickiae 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY189977254ena221725
11861Kitasatospora putterlickiae strain F18-98 16S ribosomal RNA gene, partial sequenceAY1899761532ena221725

External links

@ref: 11861

culture collection no.: DSM 44665, NCIMB 13932, Uni Bonn 18, JCM 12393, BCRC 16368, CIP 108207, IAM 15240, NBRC 100917

straininfo link

  • @ref: 83970
  • straininfo: 127995

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny14657141Kitasatospora putterlickiae sp. nov., isolated from rhizosphere soil, transfer of Streptomyces kifunensis to the genus Kitasatospora as Kitasatospora kifunensis comb. nov., and emended description of Streptomyces aureofaciens Duggar 1948.Groth I, Schutze B, Boettcher T, Pullen CB, Rodriguez C, Leistner E, Goodfellow MInt J Syst Evol Microbiol10.1099/ijs.0.02674-02003Actinomycetales/*classification/genetics/isolation & purification/physiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, *Phylogeny, Soil Microbiology, Species Specificity, Streptomyces/*classificationGenetics
Phylogeny16514020Kitasatospora sampliensis sp. nov., a novel actinobacterium isolated from soil of a sugar-cane field in India.Mayilraj S, Krishnamurthi S, Saha P, Saini HSInt J Syst Evol Microbiol10.1099/ijs.0.63836-02006Actinobacteria/*classification/genetics/isolation & purification/physiology, Carbohydrates, DNA, Ribosomal/chemistry, India, Phylogeny, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, *Soil MicrobiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11861Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44665)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44665
19687Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44665.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36705Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5804
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83970Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID127995.1StrainInfo: A central database for resolving microbial strain identifiers
122078Curators of the CIPCollection of Institut Pasteur (CIP 108207)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108207