Strain identifier

BacDive ID: 14849

Type strain: Yes

Species: Streptococcus oralis subsp. tigurinus

Strain Designation: AZ_3a

Strain history: <- A. Zbinden, Univ. Zurich,Inst. of Medical Microbiology, AZ_3a

NCBI tax ID(s): 1077464 (subspecies)

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General

@ref: 17790

BacDive-ID: 14849

DSM-Number: 24864

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped

description: Streptococcus oralis subsp. tigurinus AZ_3a is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from blood culture of endocarditis patient.

NCBI tax id

  • NCBI tax id: 1077464
  • Matching level: subspecies

strain history

  • @ref: 17790
  • history: <- A. Zbinden, Univ. Zurich,Inst. of Medical Microbiology, AZ_3a

doi: 10.13145/bacdive14849.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus oralis subsp. tigurinus
  • full scientific name: Streptococcus oralis subsp. tigurinus (Zbinden et al. 2012) Jensen et al. 2016

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus tigurinus

@ref: 17790

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus oralis subsp. tigurinus

full scientific name: Streptococcus oralis subsp. tigurinus (Zbinden et al. 2012) Jensen et al. 2016

strain designation: AZ_3a

type strain: yes

Morphology

cell morphology

  • @ref: 30537
  • gram stain: positive
  • cell width: 0.75 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 17790
  • type of hemolysis: alpha
  • hemolysis ability: 1
  • incubation period: 1-2 days

pigmentation

  • @ref: 30537
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17790TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
17790COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
17790positivegrowth37mesophilic
30537positivegrowth25-42
63342positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
17790microaerophile
30537facultative anaerobe
63342microaerophile

spore formation

  • @ref: 30537
  • spore formation: no

observation

  • @ref: 30537
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3053728757fructose+carbon source
3053728260galactose+carbon source
3053717234glucose+carbon source
3053717716lactose+carbon source
3053717306maltose+carbon source
3053737684mannose+carbon source
3053716634raffinose+carbon source
3053717992sucrose+carbon source
6838129016arginine-hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose-builds acid from
6838117992sucrose+builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan+builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
17790catalase-1.11.1.6
17790cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
17790----+/-+------++---+---+--++------
17790--+-+/-+-----++----+---+--++------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17790blood culture of endocarditis patientZurichSwitzerlandCHEEurope
25015human blood
63342Blood culture of endocarditis patientZürichSwitzerlandCHEEurope

isolation source categories

Cat1Cat2Cat3
#Infection#Inflammation
#Infection#Patient#Blood culture
#Host Body-Site#Organ#Heart

taxonmaps

  • @ref: 69479
  • File name: preview.99_6814.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_4;97_4;98_4;99_6814&stattab=map
  • Last taxonomy: Streptococcus
  • 16S sequence: JN004270
  • Sequence Identity:
  • Total samples: 13
  • aquatic counts: 3
  • animal counts: 10

Safety information

risk assessment

  • @ref: 17790
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17790
  • description: Streptococcus tigurinus strain AZ_3a 16S ribosomal RNA gene, partial sequence
  • accession: JN004270
  • length: 1476
  • database: ena
  • NCBI tax ID: 1282664

GC content

@refGC-contentmethod
1779040.0high performance liquid chromatography (HPLC)
3053740

External links

@ref: 17790

culture collection no.: DSM 24864, CCOS 600, CCUG 66514, NBRC 113012

straininfo link

  • @ref: 83962
  • straininfo: 406640

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22357776Streptococcus tigurinus sp. nov., isolated from blood of patients with endocarditis, meningitis and spondylodiscitis.Zbinden A, Mueller NJ, Tarr PE, Sproer C, Keller PM, Bloemberg GVInt J Syst Evol Microbiol10.1099/ijs.0.038299-02012Aged, Bacterial Typing Techniques, Base Composition, Blood/microbiology, DNA, Bacterial/genetics, Discitis/*microbiology, Endocarditis/*microbiology, Humans, Meningitis/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptococcus/*classification/genetics/*isolation & purificationPathogenicity
Phylogeny22760039Streptococcus tigurinus, a novel member of the Streptococcus mitis group, causes invasive infections.Zbinden A, Mueller NJ, Tarr PE, Eich G, Schulthess B, Bahlmann AS, Keller PM, Bloemberg GVJ Clin Microbiol10.1128/JCM.00849-122012Adult, Aged, Blood/microbiology, Cerebrospinal Fluid/microbiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Female, Heart Valves/microbiology, Humans, Male, Middle Aged, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, Retrospective Studies, Sequence Analysis, DNA, Streptococcal Infections/*microbiology, Streptococcus/*classification/genetics/*isolation & purificationPathogenicity
Genetics23640198Whole-Genome Sequences of Streptococcus tigurinus Type Strain AZ_3a and S. tigurinus 1366, a Strain Causing Prosthetic Joint Infection.Gizard Y, Zbinden A, Schrenzel J, Francois PGenome Announc10.1128/genomeA.00210-122013
23856340Streptococcus tigurinus is highly virulent in a rat model of experimental endocarditis.Veloso TR, Zbinden A, Andreoni F, Giddey M, Vouillamoz J, Moreillon P, Zinkernagel AS, Entenza JMInt J Med Microbiol10.1016/j.ijmm.2013.06.0062013Animals, Aorta/microbiology/pathology, *Disease Models, Animal, Endocarditis, Bacterial/*microbiology/*pathology, Endothelial Cells/microbiology, Macrophages/immunology/microbiology, Phagocytosis, Rats, Streptococcal Infections/*microbiology/*pathology, Streptococcus/immunology/*pathogenicity
Phylogeny24974898Isolation of Streptococcus tigurinus - a novel member of Streptococcus mitis group from a case of periodontitis.Dhotre SV, Mehetre GT, Dharne MS, Suryawanshi NM, Nagoba BSFEMS Microbiol Lett10.1111/1574-6968.125192014DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Humans, Molecular Sequence Data, Periodontitis/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptococcal Infections/*microbiology, Streptococcus/*classification/genetics/*isolation & purificationPathogenicity
Phylogeny26651833Streptococcus oricebi sp. nov., isolated from the oral cavity of tufted capuchin.Saito M, Shinozaki-Kuwahara N, Hirasawa M, Takada KInt J Syst Evol Microbiol10.1099/ijsem.0.0008342015Animals, Bacterial Typing Techniques, Base Composition, Cebus/*microbiology, DNA, Bacterial/genetics, DNA-Directed RNA Polymerases/genetics, Genes, Bacterial, Mouth/*microbiology, Philippines, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptococcus/*classification/genetics/isolation & purificationTranscriptome
Metabolism27505001Comparative Genomics Analysis of Streptococcus tigurinus Strains Identifies Genetic Elements Specifically and Uniquely Present in Highly Virulent Strains.Diene SM, Francois P, Zbinden A, Entenza JM, Resch GPLoS One10.1371/journal.pone.01605542016Ascorbic Acid/metabolism, Bacterial Adhesion/genetics, Biological Transport/genetics, *Genomics, Hyaluronic Acid/metabolism, Indoles/metabolism, Iron/metabolism, Molecular Sequence Annotation, Phenotype, Proteomics, Species Specificity, Streptococcus/*genetics/metabolism/*pathogenicity, Tryptophan/metabolism, Virulence/geneticsGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
17790Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24864)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24864
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
25015A. S. Jensen, C. F.,Kilian, M.10.1099/ijsem.0.001433Re-evaluation of the taxonomy of the Mitis group of the genus Streptococcus based on whole genome phylogenetic analyses, and proposed reclassification of Streptococcus dentisani as Streptococcus oralis subsp. dentisani comb. nov., Streptococcus tigurinus as Streptococcus oralis subsp. tigurinus comb. nov., and Streptococcus oligofermentans as a later synonym of Streptococcus cristatusIJSEM 66: 4803-4820 201627534397
30537Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126868
63342Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 66514)https://www.ccug.se/strain?id=66514
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83962Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID406640.1StrainInfo: A central database for resolving microbial strain identifiers