Strain identifier

BacDive ID: 14848

Type strain: Yes

Species: Streptococcus lactarius

Strain Designation: MV1

Strain history: <- J. M. Rodríguez, Univ. Complutense Madrid, Spain; MV1 <- A. Maldonado

NCBI tax ID(s): 684066 (species)

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General

@ref: 16657

BacDive-ID: 14848

DSM-Number: 23027

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped

description: Streptococcus lactarius MV1 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from breast milk .

NCBI tax id

  • NCBI tax id: 684066
  • Matching level: species

strain history

  • @ref: 16657
  • history: <- J. M. Rodríguez, Univ. Complutense Madrid, Spain; MV1 <- A. Maldonado

doi: 10.13145/bacdive14848.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus lactarius
  • full scientific name: Streptococcus lactarius Martín et al. 2011

@ref: 16657

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus lactarius

full scientific name: Streptococcus lactarius Martín et al. 2011

strain designation: MV1

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
29811positivecoccus-shapedno
69480no91.504
69480positive100

colony morphology

  • @ref: 16657
  • incubation period: 1-2 days

pigmentation

  • @ref: 29811
  • production: no

Culture and growth conditions

culture medium

  • @ref: 16657
  • name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/693
  • composition: Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
16657positivegrowth37mesophilic
63337positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16657microaerophile
29811facultative aerobe
63337anaerobe
63337microaerophile

spore formation

@refspore formationconfidence
29811no
69481no100
69480no99.955

observation

  • @ref: 29811
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2981117716lactose+carbon source
2981117306maltose+carbon source
2981117992sucrose+carbon source
298114853esculin+hydrolysis
6838129016arginine-hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose-builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
29811alkaline phosphatase+3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
16657-++--+---+--+----++-++---+--+---

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
16657breast milk (healthy woman)SpainESPEurope
63337Breast milk,healthy womanSpainESPEurope2007

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Thoracic segment
#Host Body Product#Fluids#Milk

Safety information

risk assessment

  • @ref: 16657
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16657
  • description: Streptococcus lactarius strain MV1 16S ribosomal RNA gene, partial sequence
  • accession: GU045364
  • length: 1452
  • database: ena
  • NCBI tax ID: 684066

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus lactarius CCUG 66490GCA_018127725completencbi684066
66792Streptococcus lactarius CCUG 66490GCA_016642265scaffoldncbi684066
66792Streptococcus lactarius strain CCUG 66490684066.8completepatric684066
66792Streptococcus lactarius strain CCUG 66490684066.7wgspatric684066

GC content

  • @ref: 16657
  • GC-content: 41.2
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno95.962no
flagellatedno96.52no
gram-positiveyes95.525yes
anaerobicno94.937no
aerobicno97.571no
halophileyes74.758no
spore-formingno92.473no
thermophileno99.32yes
glucose-utilyes86.294no
glucose-fermentyes87.391no

External links

@ref: 16657

culture collection no.: CCUG 66490, DSM 23027, CECT 7613

straininfo link

  • @ref: 83961
  • straininfo: 377310

literature

  • topic: Phylogeny
  • Pubmed-ID: 20511454
  • title: Streptococcus lactarius sp. nov., isolated from breast milk of healthy women.
  • authors: Martin V, Manes-Lazaro R, Rodriguez JM, Maldonado-Barragan A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.021642-0
  • year: 2010
  • mesh: DNA, Bacterial/genetics, Female, Humans, Milk, Human/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Streptococcus/*classification/genetics/*isolation & purification/metabolism
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16657Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23027)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23027
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29811Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2618828776041
63337Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 66490)https://www.ccug.se/strain?id=66490
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68381Automatically annotated from API rID32STR
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83961Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID377310.1StrainInfo: A central database for resolving microbial strain identifiers