Strain identifier

BacDive ID: 14821

Type strain: Yes

Species: Streptococcus gallolyticus

Strain history: CIP <- 1998, ACM, Streptococcus gallolyticus <- R. Osawa

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 6623

BacDive-ID: 14821

DSM-Number: 16831

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, human pathogen

description: Streptococcus gallolyticus DSM 16831 is a microaerophile, mesophilic human pathogen that was isolated from Koala, feces.

NCBI tax id

NCBI tax idMatching level
1258574strain
315405species

strain history

@refhistory
6623<- CIP <- ACM <- R. Osawa
67770R. Osawa <-- ACM 3611.
119521CIP <- 1998, ACM, Streptococcus gallolyticus <- R. Osawa

doi: 10.13145/bacdive14821.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus gallolyticus
  • full scientific name: Streptococcus gallolyticus Osawa et al. 1996
  • synonyms

    • @ref: 20215
    • synonym: Streptococcus caprinus

@ref: 6623

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus gallolyticus subsp. gallolyticus

full scientific name: Streptococcus gallolyticus subsp. gallolyticus (Osawa et al. 1996) Schlegel et al. 2003 emend. Beck et al. 2008

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no93
69480100positive
119521nopositivecoccus-shaped

colony morphology

@refincubation period
66231-2 days
119521

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6623COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
6623BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
39864MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
119521CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
6623positivegrowth37mesophilic
39864positivegrowth37mesophilic
52605positivegrowth37mesophilic
67770positivegrowth37mesophilic
119521positivegrowth10-45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6623microaerophile
52605aerobe
119521facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.988

halophily

  • @ref: 119521
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
119521esculin+hydrolysis4853
119521hippurate-hydrolysis606565
119521nitrate-reduction17632
119521nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen+builds acid from28087
68381pullulan+builds acid from27941
68381maltose+builds acid from17306
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite tests

  • @ref: 119521
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381alkaline phosphatase-3.1.3.1
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
119521oxidase-
119521beta-galactosidase-3.2.1.23
119521alcohol dehydrogenase-1.1.1.1
119521catalase-1.11.1.6
119521gamma-glutamyltransferase-2.3.2.2
119521lysine decarboxylase-4.1.1.18
119521ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119521--++-+----+++--++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119521+/----+/-----++++----++/---+++++++++++--+++-++------+/---

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
6623-+--+--+-++++---++-----+++--+---
6623-+--+--+-++++---++-----++++-+---
6623-+--+/---+-++++----+/------+++--+-+/--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
6623Koala, fecesBrisbaneAustraliaAUSAustralia and Oceania
52605Koala feces
67770Koala feces
119521Koala, fecesBrisbaneAustraliaAUSAustralia and Oceania1990

isolation source categories

Cat1Cat2Cat3
#Host#Mammals
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
6623yesyes2Risk group (German classification)
1195212Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus gallolyticus subsp. gallolyticus strain CCUG 35224 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequenceDQ204509364ena53354
20218Streptococcus gallolyticus subsp. gallolyticus strain CCUG 35224 16S-23S ribosomal RNA intergenic spacer, partial sequenceEU860360274ena53354
6623Streptococcus gallolyticus subsp. gallolyticus strain ATCC 43143 16S ribosomal RNA gene, partial sequenceAF1041141541ena981539
67770Streptococcus gallolyticus subsp. gallolyticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 10005LC1455551480ena53354
67770S.gallolyticus 16S ribsomal RNAX943371501ena53354

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus gallolyticus NCTC13773GCA_900475715completencbi315405
66792Streptococcus gallolyticus subsp. gallolyticus DSM 16831GCA_002000985completencbi1258574
66792Streptococcus gallolyticus strain NCTC13773315405.22completepatric315405
66792Streptococcus gallolyticus subsp. gallolyticus DSM 16831 strain DSM 168311258574.3completepatric1258574
66792Streptococcus gallolyticus gallolyticus DSM 168312917282946completeimg1258574

GC content

  • @ref: 67770
  • GC-content: 38.1
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno94.447no
gram-positiveyes95.356no
anaerobicno87.524no
aerobicno97.289no
halophileyes65.792no
spore-formingno91.504no
glucose-utilyes90.811no
thermophileno99.577yes
motileno92.823no
glucose-fermentyes78.992no

External links

@ref: 6623

culture collection no.: DSM 16831, ACM 3611, CCUG 35224, CIP 105428, JCM 10005, LMG 16802, CIP 105425, NCTC 13773

straininfo link

  • @ref: 83938
  • straininfo: 8029

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12054261Isolation from a shea cake digester of a tannin-degrading Streptococcus gallolyticus strain that decarboxylates protocatechuic and hydroxycinnamic acids, and emendation of the species.Chamkha M, Patel BKC, Traore A, Garcia JL, Labat MInt J Syst Evol Microbiol10.1099/00207713-52-3-9392002Anaerobiosis, Bacterial Typing Techniques, Biodegradation, Environmental, Bioreactors/*microbiology, Coumaric Acids/metabolism, DNA, Ribosomal/analysis, Decarboxylation, Hydroxybenzoates/metabolism, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptococcus/*classification/genetics/*isolation & purification/metabolism, Tannins/*metabolism, Trees/*metabolism/microbiologyMetabolism
Pathogenicity19925668Investigation into the controversial association of Streptococcus gallolyticus with colorectal cancer and adenoma.Abdulamir AS, Hafidh RR, Mahdi LK, Al-jeboori T, Abubaker FBMC Cancer10.1186/1471-2407-9-4032009Adenocarcinoma/blood/*microbiology, Adenoma/blood/*microbiology, Adult, Aged, Antibodies, Bacterial/blood, Colorectal Neoplasms/blood/*microbiology, Enzyme-Linked Immunosorbent Assay, Female, Humans, Immunoglobulin G/blood, In Situ Hybridization, Interleukin-8/biosynthesis, Male, Middle Aged, NF-kappa B/biosynthesis, RNA, Messenger/analysis, Seroepidemiologic Studies, Streptococcal Infections/*epidemiology
Genetics28428288Complete Genome Sequence of the Streptococcus gallolyticus subsp. gallolyticus Strain DSM 16831.Grimm I, Dumke J, Vollmer T, Hinse D, Ruckert C, Kalinowski J, Knabbe C, Dreier JGenome Announc10.1128/genomeA.00108-172017
Transcriptome28672015Transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in interaction with THP-1 macrophage-like cells.Grimm I, Garben N, Dreier J, Knabbe C, Vollmer TPLoS One10.1371/journal.pone.01800442017Cell Line, Gene Expression Profiling, *Genes, Bacterial, Humans, Oligonucleotide Array Sequence Analysis, Phagocytosis, Real-Time Polymerase Chain Reaction, Streptococcus gallolyticus/*genetics, *TranscriptomePathogenicity
Pathogenicity29078765Strain-dependent interactions of Streptococcus gallolyticus subsp. gallolyticus with human blood cells.Grimm I, Weinstock M, Birschmann I, Dreier J, Knabbe C, Vollmer TBMC Microbiol10.1186/s12866-017-1116-12017Cell Line, Tumor, Gene Expression Regulation/immunology, Host-Pathogen Interactions/immunology, Humans, Interleukins/blood/genetics/immunology, Macrophages/immunology, Platelet Aggregation/immunology, Species Specificity, Streptococcal Infections/blood/*immunology/*microbiology, Streptococcus gallolyticus subspecies gallolyticus/growth & development/immunology/pathogenicity/*physiology, THP-1 CellsPhylogeny
Transcriptome29373594Biofilm formation and transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in response to lysozyme.Grimm I, Dumke J, Dreier J, Knabbe C, Vollmer TPLoS One10.1371/journal.pone.01917052018*Biofilms, Muramidase/*metabolism, Streptococcus gallolyticus subspecies gallolyticus/genetics/*metabolism, *TranscriptomeMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6623Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16831)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16831
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39864Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17447
52605Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 35224)https://www.ccug.se/strain?id=35224
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83938Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8029.1StrainInfo: A central database for resolving microbial strain identifiers
119521Curators of the CIPCollection of Institut Pasteur (CIP 105428)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105428