Strain identifier

BacDive ID: 14819

Type strain: Yes

Species: Streptococcus gallolyticus subsp. macedonicus

Strain Designation: ACA-DC-206

Strain history: CIP <- 1998, F. Grimont, Inst. Pasteur, Paris, France, Streptococcus macedonicus <- LMG <- E. Tsakalidou: strain ACA-DC-206

NCBI tax ID(s): 59310 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 6133

BacDive-ID: 14819

DSM-Number: 15879

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Streptococcus gallolyticus subsp. macedonicus ACA-DC-206 is a microaerophile, mesophilic bacterium that was isolated from Greek Kasseri cheese.

NCBI tax id

  • NCBI tax id: 59310
  • Matching level: species

strain history

@refhistory
6133<- JCM <- E. Tsakalidou; ACA-DC 206
67770LMG 18488 <-- E. Tsakalidou ACA-DC 206.
119522CIP <- 1998, F. Grimont, Inst. Pasteur, Paris, France, Streptococcus macedonicus <- LMG <- E. Tsakalidou: strain ACA-DC-206

doi: 10.13145/bacdive14819.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus gallolyticus subsp. macedonicus
  • full scientific name: Streptococcus gallolyticus subsp. macedonicus (Tsakalidou et al. 1998) Schlegel et al. 2003

  • synonyms

    @refsynonym
    20215Streptococcus macedonicus
    20215Streptococcus waius

@ref: 6133

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus gallolyticus subsp. macedonicus

full scientific name: Streptococcus gallolyticus subsp. macedonicus (Tsakalidou et al. 1998) Schlegel et al. 2003

strain designation: ACA-DC-206

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.254
69480100positive
119522nopositivecoccus-shaped

colony morphology

@reftype of hemolysishemolysis abilityincubation period
6133alpha11-2 days
119522

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6133TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
38571MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
119522CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
6133positivegrowth37mesophilic
38571positivegrowth37mesophilic
67770positivegrowth37mesophilic
119522positivegrowth25-45
119522nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6133microaerophile
119522facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.994

halophily

  • @ref: 119522
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119522esculin-hydrolysis4853
119522hippurate-hydrolysis606565
119522nitrate-reduction17632
119522nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose-builds acid from27082
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
11952215688acetoin+

enzymes

@refvalueactivityec
6133catalase-1.11.1.6
6133cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
119522oxidase-
119522beta-galactosidase+3.2.1.23
119522alcohol dehydrogenase-1.1.1.1
119522catalase-1.11.1.6
119522gamma-glutamyltransferase-2.3.2.2
119522lysine decarboxylase-4.1.1.18
119522ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119522--++-+----+++--+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119522---------++++-------+/-+---+/-+/-++++---++---+--------+/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
6133--+-+----+-++---++-------+------
6133--+------+--+---++-------+------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119522---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
6133Greek Kasseri cheeseGreeceGRCEurope
55015Greek Kasseri cheese prepared from ewes' milkGreeceGRCEurope
67770Greek Kasseri cheeseGreeceGRCEurope
119522Food, Cheese, KasseriGreeceGRCEurope1998

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_1057.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_100;97_690;98_805;99_1057&stattab=map
  • Last taxonomy: Streptococcus
  • 16S sequence: LC097080
  • Sequence Identity:
  • Total samples: 43761
  • soil counts: 927
  • aquatic counts: 2522
  • animal counts: 39763
  • plant counts: 549

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
61331Risk group (German classification)
1195222Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
6133S.macedonicus DNA for 16S ribosomal RNAZ940121542ena59310
67770Streptococcus macedonicus gene for 16S ribosomal RNA, partial sequence, strain: JCM 11119LC0970801484ena59310

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus gallolyticus strain DSM 15879315405.110wgspatric315405
66792Streptococcus gallolyticus strain NCTC13767315405.31wgspatric315405
66792Streptococcus gallolyticus macedonicus DSM 158792901051635draftimg315405
67770Streptococcus gallolyticus NCTC13767GCA_900459545contigncbi315405

GC content

@refGC-contentmethod
613338
6777038high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveyes94.656no
anaerobicno94.53yes
halophileyes78.087no
spore-formingno94.783no
glucose-utilyes89.51no
flagellatedno96.578no
thermophileno99.507yes
aerobicno97.675no
motileno94.942no
glucose-fermentyes79.867no

External links

@ref: 6133

culture collection no.: CCUG 39970, ACA-DC 206, LMG LAB 617, CIP 105683, ATCC BAA 249, DSM 15879, JCM 11119, LMG 18488, NCTC 13767

straininfo link

  • @ref: 83936
  • straininfo: 12108

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9731293Identification of streptococci from Greek Kasseri cheese and description of Streptococcus macedonicus sp. nov.Tsakalidou E, Zoidou E, Pot B, Wassill L, Ludwig W, Devriese LA, Kalantzopoulos G, Schleifer KH, Kersters KInt J Syst Bacteriol10.1099/00207713-48-2-5191998Bacterial Proteins/analysis, Base Sequence, Cheese/*microbiology, DNA, Bacterial, Electrophoresis, Polyacrylamide Gel, Greece, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/analysis, RNA, Ribosomal, 16S/analysis, Sequence Analysis, RNA, Streptococcus/*classification/genetics/isolation & purification/metabolismEnzymology
Enzymology18641698Bacteriocin ST91KM, produced by Streptococcus gallolyticus subsp. macedonicus ST91KM, is a narrow-spectrum peptide active against bacteria associated with mastitis in dairy cattle.Pieterse R, Todorov SD, Dicks LMCan J Microbiol10.1139/w08-0402008Animals, Anti-Bacterial Agents/chemistry/isolation & purification/*pharmacology, Bacteriocins/chemistry/genetics/isolation & purification/*pharmacology, Cattle, Cattle Diseases/*microbiology, Female, Mastitis/microbiology/*veterinary, Peptides/chemistry/genetics/isolation & purification/pharmacology, Staphylococcus/*drug effects, Streptococcus/*drug effects/genetics/*metabolism, TemperaturePhylogeny

Reference

@idauthorscataloguedoi/urltitle
6133Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15879)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15879
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
38571Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17729
55015Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 39970)https://www.ccug.se/strain?id=39970
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83936Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12108.1StrainInfo: A central database for resolving microbial strain identifiers
119522Curators of the CIPCollection of Institut Pasteur (CIP 105683)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105683