Strain identifier

BacDive ID: 14816

Type strain: Yes

Species: Streptococcus australis

Strain Designation: AI-1

Strain history: CIP <- 2001, M. Willcox, New South Wales Univ., Sydney, Australia: strain AI-1

NCBI tax ID(s): 888833 (strain), 113107 (species)

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General

@ref: 5917

BacDive-ID: 14816

DSM-Number: 15627

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, ovoid-shaped, human pathogen

description: Streptococcus australis AI-1 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from saliva from children.

NCBI tax id

NCBI tax idMatching level
113107species
888833strain

strain history

@refhistory
5917<- ATCC <- Inst. of Dental Res. <- M. D. P. Willcox; AI-1
116649CIP <- 2001, M. Willcox, New South Wales Univ., Sydney, Australia: strain AI-1

doi: 10.13145/bacdive14816.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus australis
  • full scientific name: Streptococcus australis Willcox et al. 2001

@ref: 5917

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus australis

full scientific name: Streptococcus australis Willcox et al. 2001

strain designation: AI-1

type strain: yes

Morphology

cell morphology

  • @ref: 116649
  • gram stain: positive
  • cell shape: ovoid-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis abilityincubation period
5917alpha11-2 days
1166491

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5917COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
5917TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
40682MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
116649CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5917positivegrowth37mesophilic
40682positivegrowth37mesophilic
56982positivegrowth37mesophilic
116649positivegrowth25-41
116649nogrowth10psychrophilic
116649nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5917microaerophile
56982microaerophile
116649facultative anaerobe

halophily

  • @ref: 116649
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116649esculin-hydrolysis4853
116649hippurate+hydrolysis606565
116649nitrate-reduction17632
116649nitrite-reduction16301
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381trehalose-builds acid from27082
68381raffinose-builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

antibiotic resistance

  • @ref: 116649
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin-
11664915688acetoin-

enzymes

@refvalueactivityec
5917catalase-1.11.1.6
5917cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase+
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
116649oxidase-
116649beta-galactosidase+3.2.1.23
116649alcohol dehydrogenase-1.1.1.1
116649catalase-1.11.1.6
116649gamma-glutamyltransferase-2.3.2.2
116649lysine decarboxylase-4.1.1.18
116649ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116649--++-++---++-+-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116649---------++++--------+-++/-++++-+----+---+---------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
5917--+---------+----+---+--+/-+------
5917+++--+---+--+----+--++--++------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic locationisolation date
5917saliva from childrenAustraliaAUSAustralia and Oceania
56982Human saliva,childAustraliaAUSAustralia and Oceania1987
116649Human, Child, salivaAustraliaAUSAustralia and OceaniaSydney, New South Wales1987

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host Body Product#Oral cavity and Airways#Saliva
#Host#Human#Child

taxonmaps

  • @ref: 69479
  • File name: preview.99_646.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_4;97_4;98_523;99_646&stattab=map
  • Last taxonomy: Streptococcus
  • 16S sequence: AY485604
  • Sequence Identity:
  • Total samples: 56184
  • soil counts: 635
  • aquatic counts: 678
  • animal counts: 54450
  • plant counts: 421

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
5917yes, in single cases1Risk group (German classification)
1166492Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus australis strain ATCC 700641 16S small subunit ribosomal RNA gene, partial sequenceAY4856041471ena888833
20218Streptococcus australis ATCC 700641 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceJN181380520ena888833
5917Streptococcus australis 16S ribosomal RNA gene, partial sequenceAF1849741337ena888833

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus australis NCTC13166GCA_900476055completencbi113107
66792Streptococcus australis ATCC 700641GCA_000186465scaffoldncbi888833
66792Streptococcus australis ATCC 700641GCA_000222745contigncbi888833
66792Streptococcus australis ATCC 700641888833.3wgspatric888833
66792Streptococcus australis ATCC 700641888833.12wgspatric888833
66792Streptococcus australis strain NCTC13166113107.3completepatric113107
66792Streptococcus australis NCTC 131662808606731completeimg113107
66792Streptococcus australis ATCC 700641649990003draftimg888833

GC content

  • @ref: 5917
  • GC-content: 43.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno95.176no
gram-positiveyes92.832no
anaerobicno94.986no
aerobicno95.639yes
halophileyes55.338no
spore-formingno88.028no
thermophileno99.584yes
glucose-utilyes86.108no
motileno94.516no
glucose-fermentyes81.859no

External links

@ref: 5917

culture collection no.: CCUG 45919, CIP 107167, DSM 15627, ATCC 700641, NCTC 13166

straininfo link

  • @ref: 83933
  • straininfo: 87705

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11491323Streptococcus australis sp. nov., a novel oral streptococcus.Willcox MD, Zhu H, Knox KWInt J Syst Evol Microbiol10.1099/00207713-51-4-12772001Base Composition, Child, DNA, Bacterial/chemistry/genetics, Humans, Molecular Sequence Data, Mouth/*microbiology, New South Wales, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Streptococcus/*classification/genetics/*isolation & purification/metabolismPathogenicity
Phylogeny31570960Streptococcus koreensis sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion.Lim YK, Park SN, Shin JH, Ji S, Jo E, Chang YH, Shin Y, Paek J, Kim H, Kook JKCurr Microbiol10.1007/s00284-019-01778-62019Base Composition, DNA, Bacterial/genetics, Dental Plaque/*microbiology, Fatty Acids/chemistry, Genome, Bacterial/genetics, Humans, Nucleic Acid Hybridization, Periodontitis/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Streptococcus/chemistry/*classification/cytology/geneticsGenetics
Phylogeny33133039Streptococcus xiaochunlingii sp. nov. E24 Isolated From the Oropharynx of Healthy Chinese Children.Zou Y, Sun Y, Qi H, Liu D, Tian H, Wang N, Li XFront Microbiol10.3389/fmicb.2020.5632132020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5917Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15627)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15627
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40682Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4573
56982Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 45919)https://www.ccug.se/strain?id=45919
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83933Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID87705.1StrainInfo: A central database for resolving microbial strain identifiers
116649Curators of the CIPCollection of Institut Pasteur (CIP 107167)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107167