Strain identifier

BacDive ID: 14807

Type strain: Yes

Species: Streptococcus peroris

Strain Designation: O-66

Strain history: CIP <- 1999, JCM <- Y. Kawamura: strain GTC848 <- H. Ohkuni: strain O-66

NCBI tax ID(s): 888746 (strain), 68891 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4747

BacDive-ID: 14807

DSM-Number: 12493

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, human pathogen

description: Streptococcus peroris O-66 is a microaerophile, mesophilic human pathogen that was isolated from human tooth surface.

NCBI tax id

NCBI tax idMatching level
888746strain
68891species

strain history

@refhistory
4747<- JCM <- Y. Kawamura; GTC 848 <- H. Ohkuni; O-66
67770Y. Kawamura GTC 848 <-- H. Ohkuni O-66.
121685CIP <- 1999, JCM <- Y. Kawamura: strain GTC848 <- H. Ohkuni: strain O-66

doi: 10.13145/bacdive14807.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus peroris
  • full scientific name: Streptococcus peroris Kawamura et al. 1998

@ref: 4747

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus peroris

full scientific name: Streptococcus peroris Kawamura et al. 1998

strain designation: O-66

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no94.986
69480100positive
121685nopositivecoccus-shaped

colony morphology

@reftype of hemolysishemolysis ability
4747alpha1
1216851

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4747COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
4747TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
39406MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121685CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45
121685CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
4747positivegrowth37mesophilic
39406positivegrowth37mesophilic
54941positivegrowth37mesophilic
67770positivegrowth37mesophilic
121685positivegrowth25-41
121685nogrowth10psychrophilic
121685nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4747microaerophile
54941microaerophile
121685facultative anaerobe

spore formation

@refspore formationconfidence
69481no99
69480no99.995

halophily

  • @ref: 121685
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 4747
  • murein short key: A11.61
  • type: A3alpha L-Lys-L-Ala(L-Ser)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin+builds acid from4853
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
121685esculin-hydrolysis4853
121685hippurate-hydrolysis606565
121685nitrate-reduction17632
121685nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381trehalose-builds acid from27082
68381raffinose-builds acid from16634
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin-
12168515688acetoin-

enzymes

@refvalueactivityec
4747catalase-1.11.1.6
4747cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
121685oxidase-
121685beta-galactosidase+3.2.1.23
121685alcohol dehydrogenase-1.1.1.1
121685catalase-1.11.1.6
121685gamma-glutamyltransferase+2.3.2.2
121685lysine decarboxylase-4.1.1.18
121685ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121685-+++-+----++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121685---------++++--------+-+/-+--++-+---+----+---------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
4747-----+-----------+----+---------
4747--+--+---+--+----+---+---+------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
4747human tooth surfaceJapanJapanJPNAsia
54941Human pharynx,8-month-old female,kawasaki diseaseTokyoJapanJPNAsia1990
67770Human tooth surface
121685Human, Pharynx, Kawasaki diseaseTokyoJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways#Tooth

taxonmaps

  • @ref: 69479
  • File name: preview.99_4264.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_4;97_2591;98_3211;99_4264&stattab=map
  • Last taxonomy: Streptococcus peroris
  • 16S sequence: LC096228
  • Sequence Identity:
  • Total samples: 18088
  • soil counts: 31
  • aquatic counts: 392
  • animal counts: 17536
  • plant counts: 129

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
4747yes, in single cases1Risk group (German classification)
1216852Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
4747Streptococcus peroris gene for 16S rRNA, partial sequenceAB0083141339ena888746
67770Streptococcus peroris strain CCUG 39814 16S ribosomal RNA gene, partial sequenceEU1567721329ena888746
67770Streptococcus peroris gene for 16S ribosomal RNA, partial sequence, strain: JCM 10158LC0962281485ena68891

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus peroris ATCC 700780888746.3wgspatric888746
66792Streptococcus peroris ATCC 700780649990011draftimg888746
67770Streptococcus peroris ATCC 700780GCA_000187585scaffoldncbi888746

GC content

@refGC-contentmethod
474739.8high performance liquid chromatography (HPLC)
6777039.8-40.5high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileno95.251no
flagellatedno96.549no
gram-positiveyes93.201no
anaerobicno93.738yes
halophileyes78.476no
spore-formingno94.851no
thermophileno99.281no
glucose-utilyes86.128no
aerobicno97.871no
glucose-fermentyes86.448no

External links

@ref: 4747

culture collection no.: CCUG 39814, GTC 848, DSM 12493, ATCC 700780, JCM 10158, CCM 7374, CIP 105950, LMG 18719

straininfo link

  • @ref: 83924
  • straininfo: 12428

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9734047Streptococcus peroris sp. nov. and Streptococcus infantis sp. nov., new members of the Streptococcus mitis group, isolated from human clinical specimens.Kawamura Y, Hou XG, Todome Y, Sultana F, Hirose K, Shu SE, Ezaki T, Ohkuni HInt J Syst Bacteriol10.1099/00207713-48-3-9211998Base Composition, Base Sequence, Child, Preschool, DNA, Bacterial/analysis, DNA, Ribosomal/chemistry, Humans, Infant, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Ribosomal, 16S/genetics, Streptococcus/*classification/genetics/isolation & purificationPathogenicity
Phylogeny20511454Streptococcus lactarius sp. nov., isolated from breast milk of healthy women.Martin V, Manes-Lazaro R, Rodriguez JM, Maldonado-Barragan AInt J Syst Evol Microbiol10.1099/ijs.0.021642-02010DNA, Bacterial/genetics, Female, Humans, Milk, Human/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Streptococcus/*classification/genetics/*isolation & purification/metabolismPathogenicity

Reference

@idauthorscataloguedoi/urltitle
4747Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12493)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12493
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39406Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18026
54941Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 39814)https://www.ccug.se/strain?id=39814
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83924Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12428.1StrainInfo: A central database for resolving microbial strain identifiers
121685Curators of the CIPCollection of Institut Pasteur (CIP 105950)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105950