Strain identifier

BacDive ID: 148

Type strain: Yes

Species: Actinomyces bovis

Strain Designation: P2S, PS1

Strain history: CIP <- 1988, ATCC <- L. Pine: strain PS1

NCBI tax ID(s): 1658 (species)

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General

@ref: 10674

BacDive-ID: 148

DSM-Number: 43014

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, animal pathogen

description: Actinomyces bovis P2S is an anaerobe, mesophilic, Gram-positive animal pathogen that was isolated from lumpy jaw in cows.

NCBI tax id

  • NCBI tax id: 1658
  • Matching level: species

strain history

@refhistory
10674<- K. Schaal <- ATCC <- L. Pine, P1S
116908CIP <- 1988, ATCC <- L. Pine: strain PS1

doi: 10.13145/bacdive148.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Actinomyces
  • species: Actinomyces bovis
  • full scientific name: Actinomyces bovis Harz 1877 (Approved Lists 1980)

@ref: 10674

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Actinomyces

species: Actinomyces bovis

full scientific name: Actinomyces bovis Harz 1877

strain designation: P2S, PS1

type strain: yes

Morphology

cell morphology

  • @ref: 116908
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 116908

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40584MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
10674BHI/3 MEDIUM (DSMZ Medium 218)yesName: BHI/3 MEDIUM (DSMZ Medium 218) Composition: Brain heart infusion 37.0 g/l Casein hydrolysate 10.0 g/l Starch 1.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/218
116908CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
40584positivegrowth37mesophilic
10674positivegrowth37mesophilic
116908positivegrowth30-41
116908nogrowth10psychrophilic
116908nogrowth25mesophilic
116908nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 10674
  • oxygen tolerance: anaerobe

halophily

@refsaltgrowthtested relationconcentration
116908NaClpositivegrowth0-2 %
116908NaClnogrowth4 %
116908NaClnogrowth6 %
116908NaClnogrowth8 %
116908NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116908citrate-carbon source16947
116908esculin-hydrolysis4853
116908hippurate-hydrolysis606565
116908nitrate-reduction17632
116908nitrite-reduction16301
116908nitrate+respiration17632
68380urea-hydrolysis16199
68380D-mannose-fermentation16024
68380L-glutamate-degradation29985
68380nitrate-reduction17632
68380tryptophan-energy source27897

metabolite production

@refChebi-IDmetaboliteproduction
11690835581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6838035581indole-
11690815688acetoin-
11690817234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380urease-3.5.1.5
116908oxidase-
116908beta-galactosidase+3.2.1.23
116908alcohol dehydrogenase-1.1.1.1
116908gelatinase-
116908amylase+
116908DNase-
116908caseinase-3.4.21.50
116908catalase+1.11.1.6
116908tween esterase+
116908gamma-glutamyltransferase-2.3.2.2
116908lecithinase-
116908lysine decarboxylase-4.1.1.18
116908ornithine decarboxylase-4.1.1.17
116908tryptophan deaminase-
116908urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116908-----+-----+--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116908---+/------++++/---------+/---+/---++-+/---+/--++--+/----------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
10674-+/--+--+--+----------++-+-----
10674---+--+--+-+/------+/-+-++-+++---

Isolation, sampling and environmental information

isolation

@refsample type
10674lumpy jaw in cows
116908Animal, Cow, jaw

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Oral cavity and airways#Mouth

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
10674yes2Risk group (German classification)
1169082Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinomyces bovis partial 16S rRNAX532241367ena1658
20218A.bovis 16S rRNA geneX810611434ena1658

External links

@ref: 10674

culture collection no.: DSM 43014, ATCC 13683, CIP 103258, CCUG 18308, NCTC 11535

straininfo link

  • @ref: 69830
  • straininfo: 35859

literature

  • topic: Phylogeny
  • Pubmed-ID: 21948094
  • title: Actinomyces weissii sp. nov., isolated from dogs.
  • authors: Hijazin M, Alber J, Lammler C, Kampfer P, Glaeser SP, Busse HJ, Kassmannhuber J, Prenger-Berninghoff E, Fornges T, Hassan AA, Abdulmawjood A, Zschock M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.035626-0
  • year: 2011
  • mesh: Actinomyces/*classification/genetics/*isolation & purification, Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Dogs/*microbiology, Fatty Acids/analysis, Molecular Sequence Data, Phospholipids/analysis, *Phylogeny, Polyamines/analysis, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10674Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43014)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43014
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40584Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15036
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69830Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35859.1StrainInfo: A central database for resolving microbial strain identifiers
116908Curators of the CIPCollection of Institut Pasteur (CIP 103258)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103258