Strain identifier

BacDive ID: 14799

Type strain: Yes

Species: Streptococcus dysgalactiae

Strain Designation: 0134, 134

Strain history: CIP <- 1988, NCFB <- T.M. Higgs: strain 0134

NCBI tax ID(s): 1334 (species)

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General

@ref: 8882

BacDive-ID: 14799

DSM-Number: 20662

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, motile, coccus-shaped, animal pathogen

description: Streptococcus dysgalactiae 0134 is a microaerophile, mesophilic, Gram-positive animal pathogen that was isolated from cow.

NCBI tax id

  • NCBI tax id: 1334
  • Matching level: species

strain history

@refhistory
8882<- NCDO; NCDO 2023 <- T.M. Higgs; 0134
118987CIP <- 1988, NCFB <- T.M. Higgs: strain 0134

doi: 10.13145/bacdive14799.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus dysgalactiae
  • full scientific name: Streptococcus dysgalactiae (ex Diernhofer 1932) Garvie et al. 1983

@ref: 8882

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus dysgalactiae subsp. dysgalactiae

full scientific name: Streptococcus dysgalactiae subsp. dysgalactiae (Garvie et al. 1983) Vandamme et al. 1996 emend. Vieira et al. 1998

strain designation: 0134, 134

type strain: yes

Morphology

cell morphology

  • @ref: 118987
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: yes

colony morphology

@reftype of hemolysishemolysis abilityincubation period
8882alpha11-2 days
118987

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8882COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8882TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
39689MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
118987CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
8882positivegrowth37mesophilic
39689positivegrowth37mesophilic
48708positivegrowth37mesophilic
118987positivegrowth25-41
118987nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8882microaerophile
118987facultative anaerobe

halophily

  • @ref: 118987
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 8882
  • murein short key: A11.05
  • type: A3alpha L-Lys-L-Ala2

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837028087glycogen-builds acid from
6837028017starch+builds acid from
6837016634raffinose-builds acid from
6837015443inulin-builds acid from
6837027082trehalose+builds acid from
6837017716lactose+builds acid from
6837017924D-sorbitol-builds acid from
6837016899D-mannitol-builds acid from
6837030849L-arabinose-builds acid from
6837016988D-ribose+builds acid from
6837029016arginine+hydrolysis
683704853esculin-hydrolysis
68370606565hippurate-hydrolysis
1189874853esculin-hydrolysis
118987606565hippurate-hydrolysis
11898717632nitrate-reduction
11898716301nitrite-reduction
11898717632nitrate+respiration
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan+builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose+builds acid from
6838116199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6837015688acetoinno
11898735581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6837015688acetoin-
11898715688acetoin-

enzymes

@refvalueactivityec
8882catalase-1.11.1.6
8882cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase+3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase+3.5.3.6
68370arginine dihydrolase+3.5.3.6
68370leucine arylamidase+3.4.11.1
68370alkaline phosphatase+3.1.3.1
68370beta-galactosidase-3.2.1.23
68370beta-glucuronidase+3.2.1.31
68370alpha-galactosidase-3.2.1.22
68370pyrrolidonyl arylamidase-3.4.19.3
68370beta-glucosidase-3.2.1.21
118987oxidase-
118987beta-galactosidase+3.2.1.23
118987alcohol dehydrogenase-1.1.1.1
118987catalase-1.11.1.6
118987gamma-glutamyltransferase-2.3.2.2
118987lysine decarboxylase-4.1.1.18
118987ornithine decarboxylase-4.1.1.17
118987urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118987-+---++---++--++----

API 20STR

@refVPHIPESCPYRAalpha GALbeta GURbeta GALPALLAPADHRIBARAMANSORLACTREINURAFAMDGLYGbeta HEM
8882-----+-++++---++--+--

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANUREGTA
8882+-++-++--++-+----+-----++---+--
8882+--+-++--++-+---++-----++---+--+
8882+-++-++--++-+----+-----++---+---
8882+--+-++--++-+----+-----++---+---

Isolation, sampling and environmental information

isolation

@refsample typesampling datecountryorigin.countrycontinent
8882cow
48708Cow,experimental infection1970United KingdomGBREurope
118987Animal, Cow

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Bovinae (Cow, Cattle)

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
8882yes2Risk group (German classification)
1189872Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus dysgalactiae subsp. dysgalactiae DNA, 16S-23S rRNA intergenic spacer, partial sequenceAB258406227ena99822
20218Streptococcus dysgalactiae subsp. dysgalactiae strain ATCC 43078 16S ribosomal RNA gene, partial sequenceAY1213591313ena99822
20218Streptococcus dysgalactiae strain ATCC 43078 16S ribosomal RNA gene, partial sequenceAY5844781471ena1334
20218Streptococcus dysgalactiae ATCC 43078 16S rRNA gene, partial sequenceU02912392ena1334
8882Streptococcus dysgalactiae DNA for 16S rRNA, strain ATCC 43078AB0024851506ena1334

Genome sequences

  • @ref: 66792
  • description: Streptococcus dysgalactiae strain FDAARGOS_1157
  • accession: 1334.232
  • assembly level: complete
  • database: patric
  • NCBI tax ID: 1334

GC content

  • @ref: 8882
  • GC-content: 38.8

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno96.844no
gram-positiveyes93.582no
anaerobicno95.653yes
halophileyes84.194no
spore-formingno95.098no
thermophileno99.465yes
glucose-utilyes90.077no
flagellatedno98.385no
aerobicno93.994no
glucose-fermentyes86.581no

External links

@ref: 8882

culture collection no.: DSM 20662, ATCC 43078, NCDO 2023, CCUG 27301, LMG 16023, LMG 15885, CIP 102914

straininfo link

  • @ref: 83916
  • straininfo: 92501

literature

  • topic: Phylogeny
  • Pubmed-ID: 14657127
  • title: rDNA sequence analyses of Streptococcus dysgalactiae subsp. equisimilis isolates from pigs.
  • authors: Kawata K, Minakami T, Mori Y, Katsumi M, Kataoka Y, Ezawa A, Kikuchi N, Takahashi T
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02666-0
  • year: 2003
  • mesh: Animals, Base Sequence, DNA Primers, DNA, Bacterial/genetics, DNA, Ribosomal/*genetics, *Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Alignment, Sequence Homology, Nucleic Acid, Streptococcus/*classification/*genetics/isolation & purification, Swine/*microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8882Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20662)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20662
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39689Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14653
48708Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 27301)https://www.ccug.se/strain?id=27301
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68370Automatically annotated from API 20STR
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
83916Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92501.1StrainInfo: A central database for resolving microbial strain identifiers
118987Curators of the CIPCollection of Institut Pasteur (CIP 102914)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102914