Strain identifier

BacDive ID: 14796

Type strain: Yes

Species: Streptococcus suis

Strain Designation: S735

Strain history: CIP <- 1988, NCTC, UK, Streptococcus sp., Group R <- 1961, M.T. Parker: strain 735, Streptococcus suis 2

NCBI tax ID(s): 1307 (species)

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General

@ref: 3683

BacDive-ID: 14796

DSM-Number: 9682

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, coccus-shaped

description: Streptococcus suis S735 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from pig.

NCBI tax id

  • NCBI tax id: 1307
  • Matching level: species

strain history

@refhistory
3683<- NCTC <- M.T. Parker, Henrichsen S735
123379CIP <- 1988, NCTC, UK, Streptococcus sp., Group R <- 1961, M.T. Parker: strain 735, Streptococcus suis 2

doi: 10.13145/bacdive14796.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus suis
  • full scientific name: Streptococcus suis (ex Elliott 1966) Kilpper-Bälz and Schleifer 1987

@ref: 3683

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus suis

full scientific name: Streptococcus suis (ex Elliott 1966) Kilpper-Bälz and Schleifer 1987

strain designation: S735

type strain: yes

Morphology

cell morphology

  • @ref: 123379
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 3683
  • type of hemolysis: alpha/beta
  • hemolysis ability: 1
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3683TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
3683COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
3683STREPTOCOCCUS SUIS MEDIUM (DSMZ Medium 696)yeshttps://mediadive.dsmz.de/medium/696Name: STREPTOCOCCUS SUIS MEDIUM (DSMZ Medium 696) Composition: Bovine serum 50.0 g/l Peptone 10.0 g/l Meat extract 8.0 g/l Lactose 5.0 g/l Glucose 5.0 g/l Yeast extract 3.0 g/l K2HPO4 2.5 g/l KH2PO4 2.5 g/l L-Cysteine HCl x H2O 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x 4 H2O 0.05 g/l Distilled water
40568MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
123379CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
3683positivegrowth37mesophilic
40568positivegrowth37mesophilic
45063positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
3683anaerobe
3683microaerophile
45063aerobe
123379facultative anaerobe

murein

  • @ref: 3683
  • murein short key: A11
  • type: A1alpha L-Lys-direct

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin+builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123379nitrate-reduction17632
123379nitrite-reduction16301
68381arginine+hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen+builds acid from28087
68381pullulan+builds acid from27941
68381maltose+builds acid from17306
68381melezitose-builds acid from6731
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 123379
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
3683catalase-1.11.1.6
3683cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase+3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
123379oxidase-
123379catalase-1.11.1.6
123379urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123379--+--+-----+--+-----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123379---------++++--------+-+/-++-++-+++-+++-----------+/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
3683+++++----++++---++++++-++++-+/----
3683+++++----++++----++/-+++-++++-+---
3683+++++----++++----+-+-+-+++--+-+-
3683+++++----++++----+-+/--+-++++/--+-+/--
3683+++++----++++----+-----+++------
3683+++++----++++----+-+++-++++-+---

Isolation, sampling and environmental information

isolation

@refsample type
3683pig
45063Pig
123379Animal, Pig

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Suidae (Pig,Swine)

taxonmaps

  • @ref: 69479
  • File name: preview.99_11.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_8;97_8;98_10;99_11&stattab=map
  • Last taxonomy: Streptococcus suis subclade
  • 16S sequence: GU360732
  • Sequence Identity:
  • Total samples: 81031
  • soil counts: 1630
  • aquatic counts: 3016
  • animal counts: 75793
  • plant counts: 592

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
36832Risk group (German classification)
1233792Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus suis strain 735 16S-23S rRNA intergenic spacer region, complete sequenceAF489613406ena1307
20218Streptococcus suis strain ATCC 43765 16S ribosomal RNA gene, partial sequenceDQ3031931476ena1307
20218Streptococcus suis strain ATCC 43765 16S ribosomal RNA gene, partial sequenceGU3607321336ena1307
20218Streptococcus suis strain BCRC 14750 16S-23S ribosomal RNA intergenic spacer, partial sequenceDQ204558405ena1307
20218Streptococcus suis DNA for 16S rRNA, strain ATCC 43765, NCTC 10234AB0025251457ena1307
20218Streptococcus suis strain S735 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer and tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY5851961563ena1184252
3683Streptococcus suis 16S ribosomal RNA gene, complete sequenceAF0094771535ena1307

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus suis NCTC10234GCA_900475585completencbi1307
66792Streptococcus suis S735GCA_000294495completencbi1184252
66792Streptococcus suis S7351184252.3completepatric1184252
66792Streptococcus suis strain NCTC102341307.2047completepatric1307
66792Streptococcus suis NCTC 102342917285515completeimg1307

GC content

  • @ref: 3683
  • GC-content: 38.4
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno95.273no
gram-positiveyes94.894no
anaerobicno96.449no
aerobicno96.551no
halophileyes72.817no
spore-formingno95.47no
thermophileno99.442yes
glucose-utilyes88.878no
motileno96.517no
glucose-fermentyes86.726no

External links

@ref: 3683

culture collection no.: DSM 9682, ATCC 43765, CCUG 7984, NCTC 10234, CIP 103217

straininfo link

  • @ref: 83913
  • straininfo: 8104

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phenotype8828129Characterization of virulence of the Streptococcus suis serotype 2 reference strain Henrichsen S 735 in newborn gnotobiotic pigs.Vecht U, Wisselink HJ, Stockhofe-Zurwieden N, Smith HEVet Microbiol10.1016/0378-1135(96)00028-41996Administration, Intranasal, Animals, Animals, Newborn, Injections, Intravenous/veterinary, Phenotype, Species Specificity, Specific Pathogen-Free Organisms, Streptococcal Infections/complications/microbiology/*veterinary, Streptococcus suis/chemistry/genetics/*pathogenicity, Swine, Swine Diseases/*microbiology, VirulencePhylogeny
8915993Comparative preparation methods of sialylated capsule antigen from Streptococcus suis type 2 with type specific antigenicity.Katsumi M, Saito T, Kataoka Y, Itoh T, Kikuchi N, Hiramune TJ Vet Med Sci10.1292/jvms.58.10_9471996Antigens, Bacterial/*immunology, Bacterial Capsules/*immunology, Chromatography, Gas, Chromatography, Gel, Chromatography, High Pressure Liquid, Cross Reactions, Epitopes/immunology, Magnetic Resonance Spectroscopy, N-Acetylneuraminic Acid, Polysaccharides, Bacterial/*immunology, Streptococcus suis/*immunology
Genetics11807057Characterization of Streptococcus suis genes encoding proteins homologous to sortase of gram-positive bacteria.Osaki M, Takamatsu D, Shimoji Y, Sekizaki TJ Bacteriol10.1128/jb.184.4.971-982.20022002Amino Acid Sequence, Aminoacyltransferases/*genetics, Bacterial Proteins/*genetics/metabolism, Base Sequence, Cell Wall, Cysteine Endopeptidases, DNA Gyrase/genetics, DNA, Bacterial, *Genes, Bacterial/physiology, Gram-Positive Bacteria/genetics, Molecular Sequence Data, Mutagenesis, Sequence Analysis, Protein, Sequence Homology, Amino Acid, Staphylococcus aureus/genetics, Streptococcus/genetics, Streptococcus mutans/genetics, Streptococcus pneumoniae/genetics, Streptococcus pyogenes/genetics, Streptococcus suis/*enzymology/geneticsTranscriptome
Phylogeny12620620Allelic variation in srtAs of Streptococcus suis strains.Osaki M, Takamatsu D, Shimoji Y, Sekizaki TFEMS Microbiol Lett10.1016/S0378-1097(03)00058-22003*Alleles, Aminoacyltransferases/analysis/*genetics, Bacterial Proteins, Base Sequence, Culture Media, Cysteine Endopeptidases, *Genetic Variation, Nucleic Acid Hybridization, Phylogeny, Polymerase Chain Reaction/methods, Prevalence, Streptococcus suis/classification/*enzymology/geneticsCultivation
Phylogeny18338579[Identification and characterization of a novel infection-related factor (cell wall hydrolase/autolysin) of Streptococcus suis serotype 2].Gu H, Lu CWei Sheng Wu Xue Bao2008Animals, Bacterial Proteins/chemistry/*genetics/metabolism, Humans, N-Acetylmuramoyl-L-alanine Amidase/chemistry/*genetics/metabolism, Phylogeny, Protein Structure, Tertiary, Streptococcal Infections/*microbiology/*veterinary, Streptococcus suis/classification/*enzymology/genetics/*pathogenicity, Sus scrofa, Swine Diseases/*microbiology, VirulenceMetabolism
Phylogeny19244430Streptococcus plurextorum sp. nov., isolated from pigs.Vela AI, Casamayor A, Sanchez Del Rey V, Dominguez L, Fernandez-Garayzabal JFInt J Syst Evol Microbiol10.1099/ijs.0.65636-02009Animals, Bacterial Proteins/genetics, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, DNA-Directed RNA Polymerases/genetics, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S, Species Specificity, Streptococcal Infections/microbiology/*veterinary, Streptococcus/*classification/genetics/*isolation & purification/physiology, Superoxide Dismutase/genetics, Swine, Swine Diseases/*microbiologyGenetics
Genetics19271540[Identification and detection of trag: a new infection-related gene expressed in vivo from isolates of Streptococcus suis].Zhu H, Gu H, Lu CWei Sheng Wu Xue Bao2008Animals, China, Disease Models, Animal, Gene Expression/*genetics, Molecular Sequence Data, Streptococcal Infections/*genetics/metabolism, *Streptococcus suis/genetics/immunology/pathogenicity, SwineMetabolism
Phylogeny19648324Streptococcus porci sp. nov., isolated from swine sources.Vela AI, Perez M, Zamora L, Palacios L, Dominguez L, Fernandez-Garayzabal JFInt J Syst Evol Microbiol10.1099/ijs.0.011171-02009Animals, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Streptococcus/*classification/genetics/*isolation & purification/metabolism, Swine/*microbiologyGenetics
Phylogeny20675439Streptococcus porcorum sp. nov., isolated from domestic and wild pigs.Vela AI, Sanchez V, Mentaberre G, Lavin S, Dominguez L, Fernandez-Garayzabal JFInt J Syst Evol Microbiol10.1099/ijs.0.022657-02010Animals, Bacterial Typing Techniques, Base Composition, Chile, DNA, Bacterial/genetics, Genes, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain, Streptococcus/*classification/genetics/isolation & purification, Swine/*microbiologyGenetics
Phylogeny23245487Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22, 26, and 33 based on DNA-DNA homology and sodA and recN phylogenies.Tien LHT, Nishibori T, Nishitani Y, Nomoto R, Osawa RVet Microbiol10.1016/j.vetmic.2012.11.0012012Animal Diseases/*microbiology, Animals, DNA, Bacterial/*genetics, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Serotyping, Streptococcal Infections/microbiology/*veterinary, Streptococcus suis/*classification/geneticsTranscriptome
Phylogeny25385995Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22 and 26: Streptococcus parasuis sp. nov.Nomoto R, Maruyama F, Ishida S, Tohya M, Sekizaki T, Osawa RInt J Syst Evol Microbiol10.1099/ijs.0.067116-02014Animals, DNA, Bacterial/genetics, Genes, Bacterial, Immune Sera/immunology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saliva/immunology, Sequence Analysis, DNA, Serogroup, Streptococcus/*classification, Streptococcus suis/*classification, Swine/immunologyGenetics
Phylogeny27473166Streptococcusmarmotae sp. nov., isolated from the respiratory tract of Marmota himalayana.Niu L, Lu S, Hu S, Jin D, Lai X, Yang J, Chen C, Wang Y, Wang Y, Bai X, Lan R, Lv G, Xie Y, Ye C, Xu JInt J Syst Evol Microbiol10.1099/ijsem.0.0013502016Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Genes, Bacterial, Marmota/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Respiratory System/*microbiology, Sequence Analysis, DNA, Streptococcus/*classification/genetics/isolation & purificationTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3683Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9682)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9682
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40568Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14991
45063Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 7984)https://www.ccug.se/strain?id=7984
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83913Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8104.1StrainInfo: A central database for resolving microbial strain identifiers
123379Curators of the CIPCollection of Institut Pasteur (CIP 103217)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103217