Strain identifier
BacDive ID: 14793
Type strain:
Species: Streptococcus downei
Strain Designation: MFe28
Strain history: CIP <- 1988, NCTC <- 1981, D. Beighton: strain Mfe28, Streptococcus mutans
NCBI tax ID(s): 764290 (strain), 1317 (species)
General
@ref: 2141
BacDive-ID: 14793
DSM-Number: 5635
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic
description: Streptococcus downei MFe28 is a microaerophile, mesophilic bacterium that was isolated from dental plaque of monkey.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1317 | species |
764290 | strain |
strain history
@ref | history |
---|---|
2141 | <- NCTC <- D. Beighton, MFe28 |
67770 | LMG 14514 <-- CCUG 24890 <-- NCTC 11391 <-- D. Beighton; R. Coll. of Surg. of Engl., UK; MFe28. |
118970 | CIP <- 1988, NCTC <- 1981, D. Beighton: strain Mfe28, Streptococcus mutans |
doi: 10.13145/bacdive14793.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Streptococcaceae
- genus: Streptococcus
- species: Streptococcus downei
- full scientific name: Streptococcus downei Whiley et al. 1988
@ref: 2141
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Streptococcaceae
genus: Streptococcus
species: Streptococcus downei
full scientific name: Streptococcus downei Whiley et al. 1988
strain designation: MFe28
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 95.765 | ||
69480 | 100 | positive | ||
118970 | no | positive | coccus-shaped |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
2141 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
40572 | MEDIUM 38 - for Streptococcus and Gemella Haemolysans | yes | Distilled water make up to (1000.000 ml);Horse serum (200.000 ml);Glucose (10.000 g);Brain heart infusion agar (52.000 g) | |
118970 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
118970 | CIP Medium 38 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=38 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2141 | positive | growth | 37 | mesophilic |
40572 | positive | growth | 37 | mesophilic |
48081 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
48081 | microaerophile |
118970 | facultative anaerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.996 |
murein
- @ref: 2141
- murein short key: A11.27
- type: A3alpha L-Lys-L-Thr-L-Ala2
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | + | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | + | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | esculin | - | builds acid from | 4853 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | - | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | + | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | - | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
118970 | nitrate | - | reduction | 17632 |
118970 | nitrite | - | reduction | 16301 |
68381 | arginine | - | hydrolysis | 29016 |
68381 | D-ribose | - | builds acid from | 16988 |
68381 | D-mannitol | + | builds acid from | 16899 |
68381 | sorbitol | - | builds acid from | 30911 |
68381 | lactose | + | builds acid from | 17716 |
68381 | trehalose | + | builds acid from | 27082 |
68381 | raffinose | - | builds acid from | 16634 |
68381 | sucrose | + | builds acid from | 17992 |
68381 | L-arabinose | - | builds acid from | 30849 |
68381 | D-arabitol | - | builds acid from | 18333 |
68381 | alpha-cyclodextrin | - | builds acid from | 40585 |
68381 | hippurate | - | hydrolysis | 606565 |
68381 | glycogen | - | builds acid from | 28087 |
68381 | pullulan | - | builds acid from | 27941 |
68381 | maltose | + | builds acid from | 17306 |
68381 | melibiose | - | builds acid from | 28053 |
68381 | melezitose | - | builds acid from | 6731 |
68381 | methyl beta-D-glucopyranoside | - | builds acid from | 320055 |
68381 | D-tagatose | + | builds acid from | 16443 |
68381 | urea | - | hydrolysis | 16199 |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
118970 | 35581 | indole | no |
68381 | 15688 | acetoin | yes |
metabolite tests
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: +
enzymes
@ref | value | activity | ec |
---|---|---|---|
68381 | urease | - | 3.5.1.5 |
68381 | beta-mannosidase | - | 3.2.1.25 |
68381 | glycyl tryptophan arylamidase | - | |
68381 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68381 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68381 | beta-galactosidase | - | 3.2.1.23 |
68381 | Alanyl-Phenylalanyl-Proline arylamidase | + | |
68381 | alkaline phosphatase | - | 3.1.3.1 |
68381 | alpha-galactosidase | - | 3.2.1.22 |
68381 | beta-glucuronidase | - | 3.2.1.31 |
68381 | beta-glucosidase | - | 3.2.1.21 |
68381 | arginine dihydrolase | - | 3.5.3.6 |
118970 | oxidase | - | |
118970 | catalase | - | 1.11.1.6 |
118970 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
118970 | - | - | - | - | - | + | - | - | - | - | - | + | - | - | - | + | - | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
118970 | - | - | - | - | - | - | - | - | - | + | + | + | +/- | - | - | - | - | + | - | - | - | - | - | - | - | - | - | + | + | - | + | + | - | - | - | - | - | - | - | - | - | +/- | - | - | - | - | - | - | + |
API rID32STR
@ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2141 | - | - | - | - | - | - | - | + | - | + | + | - | + | - | - | - | + | + | - | - | - | - | - | - | - | + | - | - | - | + | - | - |
2141 | - | - | - | - | - | - | - | + | - | + | + | - | + | - | - | - | + | + | - | - | - | - | - | - | - | + | - | - | - | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location | host species |
---|---|---|---|---|---|---|
2141 | dental plaque of monkey | United Kingdom | GBR | Europe | ||
48081 | Monkey dental plaque | United Kingdom | GBR | Europe | Downe | |
67770 | Dental plaque of monkeys (Macaca fascicularis) | Macaca fascicularis | ||||
118970 | Animal, Monkey, dental plaque |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Primates |
#Host Body-Site | #Oral cavity and airways | #Plaque |
#Host Body-Site | #Oral cavity and airways | #Tooth |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
2141 | 1 | Risk group (German classification) |
118970 | 2 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptococcus downei strain ATCC 33748 16S ribosomal RNA gene, complete sequence | AY188350 | 1548 | ena | 764290 |
20218 | Streptococcus downei strain BCRC 14752 16S-23S ribosomal RNA intergenic spacer, partial sequence | DQ204520 | 472 | ena | 1317 |
20218 | Streptococcus downei partial 16S rRNA gene, strain NCTC11391 | AJ420200 | 1419 | ena | 764290 |
20218 | S.downei 16S rRNA | X58306 | 1333 | ena | 764290 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Streptococcus downei MFe28 NCTC 11391 | GCA_900459175 | contig | ncbi | 764290 |
66792 | Streptococcus downei MFe28 strain NCTC 11391 | 764290.3 | wgs | patric | 764290 |
GC content
@ref | GC-content | method |
---|---|---|
2141 | 41.3 | |
67770 | 41.3 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 95.705 | no |
flagellated | no | 96.668 | no |
gram-positive | yes | 93.731 | no |
anaerobic | no | 93.35 | yes |
halophile | yes | 72.069 | no |
spore-forming | no | 94.868 | no |
glucose-util | yes | 87.336 | no |
aerobic | no | 97.768 | yes |
thermophile | no | 99.686 | no |
glucose-ferment | yes | 82.652 | no |
External links
@ref: 2141
culture collection no.: DSM 5635, ATCC 33748, NCTC 11391, CCUG 24890, JCM 31115, BCRC 14752, CIP 103222, CNCTC 6691, KCTC 3634, LMG 14514
straininfo link
- @ref: 83910
- straininfo: 10360
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Enzymology | 1832662 | Cloning of a Streptococcus sobrinus gtf gene that encodes a glucosyltransferase which produces a high-molecular-weight water-soluble glucan. | Hanada N, Yamashita Y, Shibata Y, Sato S, Katayama T, Takehara T, Inoue M | Infect Immun | 10.1128/iai.59.10.3434-3438.1991 | 1991 | Amino Acid Sequence, *Cloning, Molecular, *Genes, Bacterial, Glucans/*biosynthesis, Glucosyltransferases/analysis/*genetics/isolation & purification, Molecular Weight, Recombinant Proteins/biosynthesis, Sequence Homology, Nucleic Acid | Phylogeny |
Enzymology | 2142479 | Analysis of the Streptococcus downei gtfS gene, which specifies a glucosyltransferase that synthesizes soluble glucans. | Gilmore KS, Russell RR, Ferretti JJ | Infect Immun | 10.1128/iai.58.8.2452-2458.1990 | 1990 | Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Bacterial, *Genes, Bacterial, Glucans/*biosynthesis, Glucosyltransferases/*genetics, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Sequence Homology, Nucleic Acid, Streptococcus/enzymology/*genetics, Streptococcus mutans/genetics, Sucrose/metabolism | Genetics |
Enzymology | 2148181 | Characterization of the product of the gtfS gene of Streptococcus downei, a primer-independent enzyme synthesizing oligo-isomaltosaccharides. | Russell RR, Gilpin ML, Hanada N, Yamashita Y, Shibata Y, Takehara T | J Gen Microbiol | 10.1099/00221287-136-8-1631 | 1990 | Blotting, Western, DNA, Bacterial/analysis, *Genes, Bacterial, Glucans/*biosynthesis, Glucosyltransferases/*genetics, Isomaltose/*metabolism, Kinetics, Plasmids, Restriction Mapping, Streptococcus/enzymology/*genetics | Metabolism |
Genetics | 8478099 | Nucleotide sequence analysis of the gtfT gene from Streptococcus sobrinus OMZ176. | Hanada N, Isobe Y, Aizawa Y, Katayama T, Sato S, Inoue M | Infect Immun | 10.1128/iai.61.5.2096-2103.1993 | 1993 | Amino Acid Sequence, Base Sequence, Binding Sites, Chromosome Mapping, *Genes, Bacterial, Glucans/chemistry, Molecular Sequence Data, Oligodeoxyribonucleotides/chemistry, Polymerase Chain Reaction, Protein Sorting Signals/chemistry, Restriction Mapping, Sequence Alignment, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Streptococcus sobrinus/*genetics | Transcriptome |
Phylogeny | 10094712 | Isolation of an active catalytic core of Streptococcus downei MFe28 GTF-I glucosyltransferase. | Monchois V, Arguello-Morales M, Russell RR | J Bacteriol | 10.1128/JB.181.7.2290-2292.1999 | 1999 | *Bacterial Proteins, Catalysis, Catalytic Domain, Escherichia coli, Gene Expression, Glucosyltransferases/*genetics, Mutagenesis, Proteins/*genetics, Streptococcus/*enzymology/genetics | Enzymology |
Phylogeny | 14746543 | Species-specific PCR method for identification of Streptococcus downei. | Igarashi T, Asaga E, Murai C, Sasa R, Goto N | Lett Appl Microbiol | 10.1111/j.1472-765x.2003.01451.x | 2004 | DNA, Bacterial/analysis/isolation & purification, Dextranase/genetics, Electrophoresis, Agar Gel, Genes, Bacterial, Polymerase Chain Reaction, Sensitivity and Specificity, Species Specificity, Streptococcus/*classification/genetics/*isolation & purification | Enzymology |
Pathogenicity | 18283515 | Identification and characterization of an antigen I/II homologous gene, pah, from Streptococcus downei. | Tamura H, Yamada A, Kato H | Curr Microbiol | 10.1007/s00284-008-9117-9 | 2008 | Adult, Antigens, Bacterial/*genetics, Bacterial Adhesion/*physiology, Bacterial Proteins/*genetics, Humans, Membrane Glycoproteins, Saliva/microbiology, Streptococcus/*genetics, Streptococcus mutans, Tooth/microbiology | |
Enzymology | 19093149 | Identification and characterization of an autolysin gene, atlh, from Streptococcus downei. | Tamura H, Yamada A, Yoshida Y, Kato H | Curr Microbiol | 10.1007/s00284-008-9336-0 | 2008 | Amino Acid Sequence, Bacterial Proteins/chemistry/*genetics, Binding Sites, Biofilms/growth & development, Catalytic Domain, Cloning, Molecular, DNA, Bacterial/chemistry/genetics, Gene Deletion, Molecular Sequence Data, Molecular Weight, N-Acetylmuramoyl-L-alanine Amidase/chemistry/*genetics, Repetitive Sequences, Amino Acid, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Streptococcus/*genetics | Genetics |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
2141 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5635) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5635 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
40572 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/14996 | ||||
48081 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 24890) | https://www.ccug.se/strain?id=24890 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68381 | Automatically annotated from API rID32STR | |||||
68382 | Automatically annotated from API zym | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
83910 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID10360.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
118970 | Curators of the CIP | Collection of Institut Pasteur (CIP 103222) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103222 |