Strain identifier

BacDive ID: 14793

Type strain: Yes

Species: Streptococcus downei

Strain Designation: MFe28

Strain history: CIP <- 1988, NCTC <- 1981, D. Beighton: strain Mfe28, Streptococcus mutans

NCBI tax ID(s): 764290 (strain), 1317 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2141

BacDive-ID: 14793

DSM-Number: 5635

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Streptococcus downei MFe28 is a microaerophile, mesophilic bacterium that was isolated from dental plaque of monkey.

NCBI tax id

NCBI tax idMatching level
1317species
764290strain

strain history

@refhistory
2141<- NCTC <- D. Beighton, MFe28
67770LMG 14514 <-- CCUG 24890 <-- NCTC 11391 <-- D. Beighton; R. Coll. of Surg. of Engl., UK; MFe28.
118970CIP <- 1988, NCTC <- 1981, D. Beighton: strain Mfe28, Streptococcus mutans

doi: 10.13145/bacdive14793.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus downei
  • full scientific name: Streptococcus downei Whiley et al. 1988

@ref: 2141

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus downei

full scientific name: Streptococcus downei Whiley et al. 1988

strain designation: MFe28

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no95.765
69480100positive
118970nopositivecoccus-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2141TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
40572MEDIUM 38 - for Streptococcus and Gemella HaemolysansyesDistilled water make up to (1000.000 ml);Horse serum (200.000 ml);Glucose (10.000 g);Brain heart infusion agar (52.000 g)
118970CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
118970CIP Medium 38yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=38

culture temp

@refgrowthtypetemperaturerange
2141positivegrowth37mesophilic
40572positivegrowth37mesophilic
48081positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
48081microaerophile
118970facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.996

murein

  • @ref: 2141
  • murein short key: A11.27
  • type: A3alpha L-Lys-L-Thr-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
118970nitrate-reduction17632
118970nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381D-tagatose+builds acid from16443
68381urea-hydrolysis16199

metabolite production

@refChebi-IDmetaboliteproduction
11897035581indoleno
6838115688acetoinyes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
118970oxidase-
118970catalase-1.11.1.6
118970urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118970-----+-----+---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118970---------++++/-----+---------++-++---------+/-------+

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
2141-------+-++-+---++-------+---+--
2141-------+-++-+---++-------+---+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationhost species
2141dental plaque of monkeyUnited KingdomGBREurope
48081Monkey dental plaqueUnited KingdomGBREuropeDowne
67770Dental plaque of monkeys (Macaca fascicularis)Macaca fascicularis
118970Animal, Monkey, dental plaque

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Primates
#Host Body-Site#Oral cavity and airways#Plaque
#Host Body-Site#Oral cavity and airways#Tooth

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
21411Risk group (German classification)
1189702Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus downei strain ATCC 33748 16S ribosomal RNA gene, complete sequenceAY1883501548ena764290
20218Streptococcus downei strain BCRC 14752 16S-23S ribosomal RNA intergenic spacer, partial sequenceDQ204520472ena1317
20218Streptococcus downei partial 16S rRNA gene, strain NCTC11391AJ4202001419ena764290
20218S.downei 16S rRNAX583061333ena764290

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus downei MFe28 NCTC 11391GCA_900459175contigncbi764290
66792Streptococcus downei MFe28 strain NCTC 11391764290.3wgspatric764290

GC content

@refGC-contentmethod
214141.3
6777041.3thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno95.705no
flagellatedno96.668no
gram-positiveyes93.731no
anaerobicno93.35yes
halophileyes72.069no
spore-formingno94.868no
glucose-utilyes87.336no
aerobicno97.768yes
thermophileno99.686no
glucose-fermentyes82.652no

External links

@ref: 2141

culture collection no.: DSM 5635, ATCC 33748, NCTC 11391, CCUG 24890, JCM 31115, BCRC 14752, CIP 103222, CNCTC 6691, KCTC 3634, LMG 14514

straininfo link

  • @ref: 83910
  • straininfo: 10360

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1832662Cloning of a Streptococcus sobrinus gtf gene that encodes a glucosyltransferase which produces a high-molecular-weight water-soluble glucan.Hanada N, Yamashita Y, Shibata Y, Sato S, Katayama T, Takehara T, Inoue MInfect Immun10.1128/iai.59.10.3434-3438.19911991Amino Acid Sequence, *Cloning, Molecular, *Genes, Bacterial, Glucans/*biosynthesis, Glucosyltransferases/analysis/*genetics/isolation & purification, Molecular Weight, Recombinant Proteins/biosynthesis, Sequence Homology, Nucleic AcidPhylogeny
Enzymology2142479Analysis of the Streptococcus downei gtfS gene, which specifies a glucosyltransferase that synthesizes soluble glucans.Gilmore KS, Russell RR, Ferretti JJInfect Immun10.1128/iai.58.8.2452-2458.19901990Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Bacterial, *Genes, Bacterial, Glucans/*biosynthesis, Glucosyltransferases/*genetics, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Sequence Homology, Nucleic Acid, Streptococcus/enzymology/*genetics, Streptococcus mutans/genetics, Sucrose/metabolismGenetics
Enzymology2148181Characterization of the product of the gtfS gene of Streptococcus downei, a primer-independent enzyme synthesizing oligo-isomaltosaccharides.Russell RR, Gilpin ML, Hanada N, Yamashita Y, Shibata Y, Takehara TJ Gen Microbiol10.1099/00221287-136-8-16311990Blotting, Western, DNA, Bacterial/analysis, *Genes, Bacterial, Glucans/*biosynthesis, Glucosyltransferases/*genetics, Isomaltose/*metabolism, Kinetics, Plasmids, Restriction Mapping, Streptococcus/enzymology/*geneticsMetabolism
Genetics8478099Nucleotide sequence analysis of the gtfT gene from Streptococcus sobrinus OMZ176.Hanada N, Isobe Y, Aizawa Y, Katayama T, Sato S, Inoue MInfect Immun10.1128/iai.61.5.2096-2103.19931993Amino Acid Sequence, Base Sequence, Binding Sites, Chromosome Mapping, *Genes, Bacterial, Glucans/chemistry, Molecular Sequence Data, Oligodeoxyribonucleotides/chemistry, Polymerase Chain Reaction, Protein Sorting Signals/chemistry, Restriction Mapping, Sequence Alignment, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Streptococcus sobrinus/*geneticsTranscriptome
Phylogeny10094712Isolation of an active catalytic core of Streptococcus downei MFe28 GTF-I glucosyltransferase.Monchois V, Arguello-Morales M, Russell RRJ Bacteriol10.1128/JB.181.7.2290-2292.19991999*Bacterial Proteins, Catalysis, Catalytic Domain, Escherichia coli, Gene Expression, Glucosyltransferases/*genetics, Mutagenesis, Proteins/*genetics, Streptococcus/*enzymology/geneticsEnzymology
Phylogeny14746543Species-specific PCR method for identification of Streptococcus downei.Igarashi T, Asaga E, Murai C, Sasa R, Goto NLett Appl Microbiol10.1111/j.1472-765x.2003.01451.x2004DNA, Bacterial/analysis/isolation & purification, Dextranase/genetics, Electrophoresis, Agar Gel, Genes, Bacterial, Polymerase Chain Reaction, Sensitivity and Specificity, Species Specificity, Streptococcus/*classification/genetics/*isolation & purificationEnzymology
Pathogenicity18283515Identification and characterization of an antigen I/II homologous gene, pah, from Streptococcus downei.Tamura H, Yamada A, Kato HCurr Microbiol10.1007/s00284-008-9117-92008Adult, Antigens, Bacterial/*genetics, Bacterial Adhesion/*physiology, Bacterial Proteins/*genetics, Humans, Membrane Glycoproteins, Saliva/microbiology, Streptococcus/*genetics, Streptococcus mutans, Tooth/microbiology
Enzymology19093149Identification and characterization of an autolysin gene, atlh, from Streptococcus downei.Tamura H, Yamada A, Yoshida Y, Kato HCurr Microbiol10.1007/s00284-008-9336-02008Amino Acid Sequence, Bacterial Proteins/chemistry/*genetics, Binding Sites, Biofilms/growth & development, Catalytic Domain, Cloning, Molecular, DNA, Bacterial/chemistry/genetics, Gene Deletion, Molecular Sequence Data, Molecular Weight, N-Acetylmuramoyl-L-alanine Amidase/chemistry/*genetics, Repetitive Sequences, Amino Acid, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Streptococcus/*geneticsGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2141Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5635)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5635
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40572Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14996
48081Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 24890)https://www.ccug.se/strain?id=24890
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83910Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10360.1StrainInfo: A central database for resolving microbial strain identifiers
118970Curators of the CIPCollection of Institut Pasteur (CIP 103222)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103222