Strain identifier

BacDive ID: 14787

Type strain: Yes

Species: Streptococcus uberis

Strain history: CIP <- 1988, NCTC <- K. Diernhofer, type I

NCBI tax ID(s): 1349 (species)

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General

@ref: 8919

BacDive-ID: 14787

DSM-Number: 20569

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped, human pathogen

description: Streptococcus uberis DSM 20569 is a microaerophile, mesophilic, Gram-positive human pathogen of the family Streptococcaceae.

NCBI tax id

  • NCBI tax id: 1349
  • Matching level: species

strain history

@refhistory
8919<- NCTC; NCTC 3858 <- K. Diernhofer;
67770DSM 20569 <-- NCTC 3858 <-- K. Diernhofer.
123705CIP <- 1988, NCTC <- K. Diernhofer, type I

doi: 10.13145/bacdive14787.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus uberis
  • full scientific name: Streptococcus uberis Diernhofer 1932 (Approved Lists 1980)

@ref: 8919

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus uberis

full scientific name: Streptococcus uberis Diernhofer 1932

type strain: yes

Morphology

cell morphology

  • @ref: 123705
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysisincubation periodhemolysis ability
8919gamma1-2 days
1237051

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8919COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8919TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
41694MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123705CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
8919positivegrowth37mesophilic
41694positivegrowth37mesophilic
67770positivegrowth37mesophilic
123705positivegrowth30mesophilic
123705nogrowth10psychrophilic
123705nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8919microaerophile
123705facultative anaerobe

spore formation

  • @ref: 123705
  • spore formation: no

halophily

  • @ref: 123705
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 8919
  • murein short key: A11.05
  • type: A3alpha L-Lys-L-Ala2

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837028087glycogen-builds acid from
6837028017starch-builds acid from
6837016634raffinose-builds acid from
6837015443inulin+builds acid from
6837027082trehalose+builds acid from
6837017716lactose+builds acid from
6837017924D-sorbitol+builds acid from
6837016899D-mannitol+builds acid from
6837030849L-arabinose-builds acid from
6837016988D-ribose+builds acid from
6837029016arginine+hydrolysis
683704853esculin+hydrolysis
68370606565hippurate+hydrolysis
1237054853esculin+hydrolysis
12370517632nitrate-builds gas from
12370517632nitrate-reduction
12370516301nitrite-reduction
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol+builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate+hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
6837015688acetoinyes
12370535581indoleno
6838115688acetoinyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
6837015688acetoin+
12370515688acetoin-

enzymes

@refvalueactivityec
8919cytochrome-c oxidase-1.9.3.1
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase+3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
68370arginine dihydrolase+3.5.3.6
68370leucine arylamidase+3.4.11.1
68370alkaline phosphatase+3.1.3.1
68370beta-galactosidase-3.2.1.23
68370alpha-galactosidase-3.2.1.22
68370pyrrolidonyl arylamidase+3.4.19.3
68370beta-glucosidase+3.2.1.21
123705oxidase-
123705beta-galactosidase+3.2.1.23
123705alcohol dehydrogenase+1.1.1.1
123705catalase-1.11.1.6
123705lysine decarboxylase-4.1.1.18
123705ornithine decarboxylase-4.1.1.17
123705urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123705-+-+-+----++--+++---

API 20STR

@refVPHIPESCPYRAalpha GALbeta GURbeta GALPALLAPADHRIBARAMANSORLACTREINURAFAMDGLYGbeta HEM
8919++++-+-++++-+++++---
8919++++---++++-+++++---
8919++++-+-++++-+++++----

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
8919++-+-++++++-+---++--+++--+--+-+-
8919++++-++++++-+---++-++-+--+--+-+-
8919++++-++++++-+---++-++-+--+--+-+-
8919++++-++++++-+---++-++++--+--+-+-
8919++++-++++++-+---++-++++--+--+-+-
8919++++-++++++-+---+++/-++++--+--+-++/-
8919++++-++++++-+---++-++++--+--+-+-
8919++++--+++++-+---++----+--+--+---
8919++++--+++++-+---++----+--+--+---
8919++++-++++++-+---++-++++--+--+-+-

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
41694VienneAustriaAUTEurope
123705ViennaAustriaAUTEuropeUnknown source1933

taxonmaps

  • @ref: 69479
  • File name: preview.99_2664.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_79;97_1693;98_2063;99_2664&stattab=map
  • Last taxonomy: Streptococcus uberis subclade
  • 16S sequence: LC071829
  • Sequence Identity:
  • Total samples: 26016
  • soil counts: 829
  • aquatic counts: 630
  • animal counts: 24413
  • plant counts: 144

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
8919yesyes2Risk group (German classification)
1237052Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus uberis 16S-23S ribosomal RNA intergenic spacer, complete sequence, 23S ribosomal RNA partial sequenceAF255657585ena1349
20218Streptococcus uberis strain ATCC 19436 16S-23S ribosomal RNA intergenic spacer, complete sequenceAY347538338ena1349
20218Streptococcus uberis strain ATCC 19436 16S ribosomal RNA gene, partial sequenceJN630842803ena1349
8919Streptococcus uberis gene for 16S rRNA, strain:JCM 5709AB0235731501ena1349
67770Streptococcus uberis DNA for 16S rRNA, strain ATCC 19436, NCTC 3858AB0025261442ena1349
67770Streptococcus uberis gene for 16S ribosomal RNA, partial sequence, strain: JCM 5709LC0718291481ena1349

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus uberis NCTC3858GCA_900475595completencbi1349
66792Streptococcus uberis strain NCTC38581349.243completepatric1349
66792Streptococcus uberis NCTC 38582870424623completeimg1349

GC content

@refGC-contentmethod
891937.5
6777036.5thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno97.162no
gram-positiveyes94.049no
anaerobicno95.403yes
aerobicno95.935yes
halophileyes85.862no
spore-formingno94.905no
thermophileno99.313yes
glucose-utilyes88.67no
motileno95.655no
glucose-fermentyes83.997no

External links

@ref: 8919

culture collection no.: DSM 20569, ATCC 19436, CCUG 17930, JCM 5709, NCDO 2038, NCTC 3858, BCRC 12579, CCUG 27579, CECT 994, CIP 103219, LMG 9465, NCIMB 702038

straininfo link

  • @ref: 83904
  • straininfo: 125888

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
8434140An R-like protein of Streptococcus uberis stimulates opsonising antibodies.Groschup MH, Timoney JFRes Vet Sci10.1016/0034-5288(93)90021-71993Animals, Antibodies, Bacterial/*blood/immunology, Antigens, Bacterial/*immunology, Bacterial Proteins/*immunology, Cattle, Guinea Pigs, Opsonin Proteins/*blood/immunology, Streptococcus/*immunology
10024545Purification and cloning of a streptokinase from Streptococcus uberis.Johnsen LB, Poulsen K, Kilian M, Petersen TEInfect Immun10.1128/IAI.67.3.1072-1078.19991999Amino Acid Sequence, Animals, Bacterial Vaccines/immunology, Base Sequence, Cattle, Cloning, Molecular, Molecular Sequence Data, Plasminogen Activators/genetics/*isolation & purification, Streptococcus/*enzymology/immunology, Streptokinase/genetics/immunology/*isolation & purificationEnzymologyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8919Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20569)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20569
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41694Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14993
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68370Automatically annotated from API 20STR
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83904Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID125888.1StrainInfo: A central database for resolving microbial strain identifiers
123705Curators of the CIPCollection of Institut Pasteur (CIP 103219)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103219