Strain identifier

BacDive ID: 1477

Type strain: Yes

Species: Virgibacillus marismortui

Strain Designation: 123

Strain history: CIP <- 1998, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain 123

NCBI tax ID(s): 79660 (species)

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General

@ref: 4692

BacDive-ID: 1477

DSM-Number: 12325

keywords: 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Virgibacillus marismortui 123 is an obligate aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from water samples taken in 1936 by B.E. Volcani, inocula taken from samples in 1993.

NCBI tax id

  • NCBI tax id: 79660
  • Matching level: species

strain history

@refhistory
4692<- A. Ventosa, Univ. of Sevilla; 123
402451998, A. Ventosa, Fac. Pharm., Univ. Sevilla, Spain: strain 123
120833CIP <- 1998, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain 123

doi: 10.13145/bacdive1477.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Virgibacillus
  • species: Virgibacillus marismortui
  • full scientific name: Virgibacillus marismortui (Arahal et al. 1999) Heyrman et al. 2003

  • synonyms

    @refsynonym
    20215Salibacillus marismortui
    20215Bacillus marismortui

@ref: 4692

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Virgibacillus

species: Virgibacillus marismortui

full scientific name: Virgibacillus marismortui (Arahal et al. 1999) Heyrman et al. 2003

strain designation: 123

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
23063positive2.0-3.6 µm0.5-0.7 µmrod-shapedyes
120833positiverod-shapedyes

colony morphology

@refcolony colorcolony shape
23063creamcircular
120833

pigmentation

  • @ref: 23063
  • production: yes
  • color: cream

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4692NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
4692BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
40245Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120833CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
4692positivegrowth30mesophilic
23063positivegrowth15.0-50.0
23063positiveoptimum37.0mesophilic
40245positivegrowth37mesophilic
120833positivegrowth22-45
120833nogrowth10psychrophilic
120833nogrowth55thermophilic

culture pH

@refabilitytypepHPH range
23063positivegrowth6.0-9.0alkaliphile
23063positiveoptimum7.5
120833positivegrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23063obligate aerobe
120833obligate aerobe

spore formation

@refspore descriptiontype of sporespore formation
23063oval,terminal or subterminal,in swollen sporangiaendosporeyes
120833yes

halophily

@refsaltgrowthtested relationconcentration
120833NaClpositivegrowth2-10 %
120833NaClnogrowth0 %

murein

  • @ref: 23063
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2306317108D-arabinose-builds acid from
2306312936D-galactose-builds acid from
2306316899D-mannitol-builds acid from
2306316551D-trehalose-builds acid from
2306365327D-xylose-builds acid from
2306317716lactose-builds acid from
2306317992sucrose-builds acid from
2306316947citrate-carbon source
2306327897tryptophan-energy source
2306328017starch-hydrolysis
2306353426tween 80-hydrolysis
2306316301nitrite-reduction
2306315824D-fructose+builds acid from
2306317634D-glucose+builds acid from
2306317754glycerol+builds acid from
2306317306maltose+builds acid from
2306317234glucose+fermentation
23063casein+hydrolysis
2306316991dna+hydrolysis
230635291gelatin+hydrolysis
2306317632nitrate+reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
12083316947citrate-carbon source
1208334853esculin+hydrolysis
120833606565hippurate-hydrolysis
12083317632nitrate+reduction
12083316301nitrite-reduction
12083317632nitrate-respiration

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
23063100147nalidixic acidyesyes
230637507neomycinyesyes
2306328368novobiocinyesyes
2306328077rifampicinyesyes
2306317698chloramphenicolyesyes
2306348923erythromycinyesyes
2306318208penicillin gyesyes
2306317076streptomycinyesyes
2306327902tetracyclineyesyes

metabolite production

@refChebi-IDmetaboliteproduction
2306335581indoleno
2306316136hydrogen sulfideno
2306315688acetoinno
12083335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-testindole testcitrate test
2306315688acetoin-
2306317234glucose+
2306335581indole-
2306316947citrate-
12083315688acetoin-
12083317234glucose+

enzymes

@refvalueactivityec
23063arginine dihydrolase-3.5.3.6
23063catalase+1.11.1.6
23063cytochrome oxidase+1.9.3.1
23063phenylalanine deaminase-4.3.1.5
23063tryptophan deaminase-4.1.99.1
23063urease+3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120833oxidase+
120833beta-galactosidase+3.2.1.23
120833alcohol dehydrogenase-1.1.1.1
120833gelatinase+
120833amylase-
120833DNase+
120833caseinase+3.4.21.50
120833catalase+1.11.1.6
120833tween esterase-
120833gamma-glutamyltransferase-2.3.2.2
120833lecithinase-
120833lipase-
120833lysine decarboxylase-4.1.1.18
120833ornithine decarboxylase-4.1.1.17
120833phenylalanine ammonia-lyase-4.3.1.24
120833protease+
120833tryptophan deaminase-
120833urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120833-+++------+++-------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120833+---+-----+++--------++/-+++++---+---+/-+/---+------+/---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120833+++++--+-+++---+++++++++-------+-+++-+---+-------++-+++-+--++-----------++-+---++-----+-+-+++++-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment culture temperatureisolation date
4692water samples taken in 1936 by B.E. Volcani, inocula taken from samples in 1993Dead Sea, Northern basin, close to mouth of Jordan riverJordanJORAsia
2306337.0
120833Environment, Water sampleDead SeaIsraelISRAsia1994

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic
#Environmental#Terrestrial#Volcanic

taxonmaps

  • @ref: 69479
  • File name: preview.99_15704.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1861;97_2610;98_11847;99_15704&stattab=map
  • Last taxonomy: Virgibacillus
  • 16S sequence: AJ009793
  • Sequence Identity:
  • Total samples: 121
  • soil counts: 22
  • aquatic counts: 31
  • animal counts: 63
  • plant counts: 5

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
46921Risk group (German classification)
1208331Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Virgibacillus marismortui gene for 16S ribosomal RNA, 23S ribosomal RNA, tRNA-Ileu, tRNA-Ala, partial and complete sequence, clone: 900AB243786795ena79660
20218Virgibacillus marismortui gene for 16S ribosomal RNA, ITS1, 23S ribosomal RNA, partial and complete sequence, clone: 800AB243787688ena79660
20218Virgibacillus marismortui gene for 16S ribosomal RNA, ITS1, 23S ribosomal RNA, partial and complete sequence, clone: 700AB243788688ena79660
20218Virgibacillus marismortui isolate Sm2 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU723664643ena79660
20218Virgibacillus marismortui isolate Sm51 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU723665750ena79660
4692Bacillus marismortui strain 123, 16S ribosomal RNAAJ0097931563ena79660

GC content

  • @ref: 23063
  • GC-content: 40.7
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 4692

culture collection no.: DSM 12325, ATCC 700626, CECT 5066, CIP 105609

straininfo link

  • @ref: 71123
  • straininfo: 12957

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10319473Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea.Arahal DR, Marquez MC, Volcani BE, Schleifer KH, Ventosa AInt J Syst Bacteriol10.1099/00207713-49-2-5211999Bacillus/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Oceans and Seas, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sodium Chloride/pharmacology, Spores, Bacterial/physiology, *Water MicrobiologyGenetics
Phylogeny17473231Virgibacillus olivae sp. nov., isolated from waste wash-water from processing of Spanish-style green olives.Quesada T, Aguilera M, Morillo JA, Ramos-Cormenzana A, Monteoliva-Sanchez MInt J Syst Evol Microbiol10.1099/ijs.0.64550-02007Bacillaceae/chemistry/*classification/genetics/*isolation & purification, Base Composition, Carbohydrate Metabolism, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, *Food Microbiology, Genes, rRNA/genetics, Gentian Violet, Molecular Sequence Data, Nucleic Acid Hybridization, Olea/microbiology, Phenazines, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Water MicrobiologyBiotechnology
Phylogeny20127463Virgibacillus xinjiangensis sp. nov., isolated from a Salt Lake of Xin-jiang Province in China.Jeon CO, Kim JM, Park DJ, Xu LH, Jiang CL, Kim CJJ Microbiol10.1007/s12275-009-0107-92010Bacillaceae/chemistry/*classification/genetics/*isolation & purification, Base Composition, Cell Wall/chemistry, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Molecular Sequence Data, Peptidoglycan/analysis, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Water MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4692Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12325)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12325
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23063David R. Arahal,M. Carmen Márquez,Benjamin E. Volcani,Karl H. Schleifer,Antonio Ventosa10.1099/00207713-49-2-521Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead SeaIJSEM 49: 521-530 199910319473
40245Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17648
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71123Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12957.1StrainInfo: A central database for resolving microbial strain identifiers
120833Curators of the CIPCollection of Institut Pasteur (CIP 105609)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105609