Strain identifier

BacDive ID: 14732

Type strain: Yes

Species: Streptococcus intermedius

Strain Designation: 1877

Strain history: CIP <- 1988, NCFB <- W.E.C. Moore, Blacksburg, USA: strain VPI 3372A, <- A. Prévot, Inst. Pasteur, Paris, France: strain 1877

NCBI tax ID(s): 1095731 (strain), 1338 (species)

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General

@ref: 8923

BacDive-ID: 14732

DSM-Number: 20573

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, human pathogen

description: Streptococcus intermedius 1877 is a microaerophile, mesophilic human pathogen of the family Streptococcaceae.

NCBI tax id

NCBI tax idMatching level
1338species
1095731strain

strain history

@refhistory
8923<- ATCC; ATCC 27335 <- W. E. C. Moore; VPI 3372A <- A. Prevot; 1877
67770CCUG 32759 <-- NCFB 2227 <-- VPI 3372-A <-- A. Prévot 1877.
120155CIP <- 1988, NCFB <- W.E.C. Moore, Blacksburg, USA: strain VPI 3372A, <- A. Prévot, Inst. Pasteur, Paris, France: strain 1877

doi: 10.13145/bacdive14732.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus intermedius
  • full scientific name: Streptococcus intermedius Prévot 1925 (Approved Lists 1980)

@ref: 8923

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus intermedius

full scientific name: Streptococcus intermedius Prévot 1925 emend. Whiley and Beighton 1991

strain designation: 1877

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no95.302
69480100positive
120155nopositivecoccus-shaped

colony morphology

@reftype of hemolysisincubation period
8923gamma1-2 days
120155

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8923COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8923TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
41341MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
120155CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
8923positivegrowth37mesophilic
41341positivegrowth37mesophilic
67770positivegrowth37mesophilic
120155positivegrowth25-37mesophilic
120155nogrowth10psychrophilic
120155nogrowth41thermophilic
120155nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8923microaerophile
120155facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.989

halophily

  • @ref: 120155
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 8923
  • murein short key: A11.05
  • type: A3alpha L-Lys-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68370glycogen-builds acid from28087
68370starch+builds acid from28017
68370raffinose-builds acid from16634
68370inulin-builds acid from15443
68370trehalose+builds acid from27082
68370lactose+builds acid from17716
68370D-sorbitol-builds acid from17924
68370D-mannitol-builds acid from16899
68370L-arabinose-builds acid from30849
68370D-ribose-builds acid from16988
68370arginine+hydrolysis29016
68370esculin+hydrolysis4853
68370hippurate-hydrolysis606565
120155esculin-hydrolysis4853
120155hippurate+hydrolysis606565
120155nitrate-reduction17632
120155nitrite-reduction16301
68381arginine+hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan+builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381D-tagatose-builds acid from16443

metabolite production

@refChebi-IDmetaboliteproduction
6837015688acetoinyes
6838115688acetoinyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
6837015688acetoin+
12015515688acetoin+

enzymes

@refvalueactivityec
8923catalase-1.11.1.6
8923cytochrome-c oxidase-1.9.3.1
68381pyrrolidonyl arylamidase-3.4.19.3
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
68370arginine dihydrolase+3.5.3.6
68370leucine arylamidase+3.4.11.1
68370alkaline phosphatase+3.1.3.1
68370beta-galactosidase-3.2.1.23
68370beta-glucuronidase-3.2.1.31
68370alpha-galactosidase-3.2.1.22
68370pyrrolidonyl arylamidase-3.4.19.3
68370beta-glucosidase+3.2.1.21
120155oxidase-
120155beta-galactosidase+3.2.1.23
120155alcohol dehydrogenase+1.1.1.1
120155catalase-1.11.1.6
120155gamma-glutamyltransferase-2.3.2.2
120155lysine decarboxylase-4.1.1.18
120155ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    51165C12:07.312
    51165C14:015.714
    51165C16:030.616
    51165C15:0 3OH3.216.504
    51165C16:1 ω7c8.915.819
    51165C16:1 ω9c3.615.774
    51165C18:1 ω7c /12t/9t13.317.824
    51165C18:1 ω9c417.769
    51165C18:2 ω6,9c/C18:0 ANTE13.517.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120155-+---+++--++-+-+-+--

API 20STR

@refVPHIPESCPYRAalpha GALbeta GURbeta GALPALLAPADHRIBARAMANSORLACTREINURAFAMDGLYG
8923+-+----+++----++--+-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120155---------++++-------+++++++++-++---++-++---------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
8923+++--+---++-+---++--+/-+--++--+--+
8923+++--+---++-+---+++-++--++--+-++/-
8923+++--+---++-+---+++-+---++--+---
8923+++--+---++-+---+++/--+/----++------

Isolation, sampling and environmental information

isolation

  • @ref: 67770

taxonmaps

  • @ref: 69479
  • File name: preview.99_751.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_456;97_522;98_600;99_751&stattab=map
  • Last taxonomy: Streptococcus intermedius subclade
  • 16S sequence: AF104671
  • Sequence Identity:
  • Total samples: 90126
  • soil counts: 993
  • aquatic counts: 1629
  • animal counts: 86899
  • plant counts: 605

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
8923yesyes2Risk group (German classification)
1201552Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus intermedius strain ATCC 27335 16S-23S ribosomal RNA intergenic spacer, complete sequenceAY347549270ena1338
20218Streptococcus intermedius strain ATCC 27335 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceJN181386522ena1338
20218Streptococcus intermedius ATCC 27335 16S rRNA gene, partial sequenceU02916405ena1338
20218S.intermedius 16S ribosomal RNAZ69040509ena1338
8923Streptococcus intermedius strain ATCC27335 16S ribosomal RNA gene, partial sequenceAF1046711558ena1338

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus intermedius NCTC11324GCA_900475975completencbi1338
66792Streptococcus intermedius SK54 = ATCC 27335GCA_000258445contigncbi1095731
66792Streptococcus intermedius ATCC 273351316583.3wgspatric1095731
66792Streptococcus intermedius strain NCTC113241338.42completepatric1338
66792Streptococcus intermedius SK54, ATCC 273352537561861draftimg1095731
66792Streptococcus intermedius ATCC 273352541047048draftimg1095731
66792Streptococcus intermedius SK54 = ATCC 27335GCA_000413475contigncbi1095731

GC content

  • @ref: 8923
  • GC-content: 38.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno83no
flagellatedno97.705no
gram-positiveyes92.236no
anaerobicno90.117yes
aerobicno97.526no
halophileyes87.861no
spore-formingno94.303no
thermophileno99.485no
glucose-utilyes89.878no
motileno97.035no
glucose-fermentyes87.733no

External links

@ref: 8923

culture collection no.: DSM 20573, ATCC 27335, CIP 103248, CCUG 32759, NCTC 11324, CCUG 17827, JCM 12996, VPI 3372A, CECT 803, LMG 14510, LMG 17840, NCFB 2227, VPI 3372-A, NCDO 2227

straininfo link

  • @ref: 83850
  • straininfo: 10353

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity1995029Emended descriptions and recognition of Streptococcus constellatus, Streptococcus intermedius, and Streptococcus anginosus as distinct species.Whiley RA, Beighton DInt J Syst Bacteriol10.1099/00207713-41-1-11991Base Composition, DNA, Bacterial/analysis, Humans, Serotyping, Streptococcus/*classification/cytology/genetics/physiologyPhylogeny
Pathogenicity2111871Serogrouping of oral Streptococcus intermedius.Osano E, Tanaka T, Ozeki M, Makita I, Moriyama TMicrobiol Immunol1990Animals, Antigens, Bacterial/*analysis, Bacterial Typing Techniques, Humans, Immunodiffusion, Male, Mouth Diseases/complications/*microbiology, Rabbits, Streptococcal Infections/complications/*immunology, Streptococcus/*classification/immunology/isolation & purificationPhylogeny
Phylogeny2130861Genetic and physiological characteristics of oral Streptococcus intermedius.Osano E, Fujii Y, Hibi EAichi Gakuin Dent Sci1990Chromatography, High Pressure Liquid, DNA, Bacterial/analysis, Nucleic Acid Hybridization, Streptococcus/*classification/genetics/metabolismMetabolism
Metabolism2498150Chondroitinase C activity of Streptococcus intermedius.Hibi E, Osano E, Fujii Y, Moriyama TFEMS Microbiol Lett10.1111/j.1574-6968.1989.tb03284.x1989Chondroitin Lyases/*metabolism, Chondroitin Sulfates, Chondroitinases and Chondroitin Lyases/*metabolism, Dermatan Sulfate, Streptococcus/*enzymology, Substrate SpecificityEnzymology
Pathogenicity2610299[Taxonomic studies of the Streptococcus intermedius strains isolated from human oral cavities].Fujii YAichi Gakuin Daigaku Shigakkai Shi1989Humans, Mouth/*microbiology, Streptococcus/*classificationPhylogeny
Pathogenicity3444428Chondroitin sulfate-depolymerizing activity in Streptococcus intermedius and other streptococci.Osano E, Hibi E, Ozeki M, Fujii Y, Moriyama TMicrobiol Immunol10.1111/j.1348-0421.1987.tb01346.x1987Chondroitinases and Chondroitin Lyases/*biosynthesis, Chondroitinsulfatases/*biosynthesis, Dental Plaque/microbiology, Dental Pulp Diseases/microbiology, Humans, Streptococcal Infections/microbiology, Streptococcus/*enzymology/isolation & purificationEnzymology
Phylogeny8784576Rapid species identification of "Streptococcus milleri" strains by line blot hybridization: identification of a distinct 16S rRNA population closely related to Streptococcus constellatus.Jacobs JA, Schot CS, Bunschoten AE, Schouls LMJ Clin Microbiol10.1128/JCM.34.7.1717-1721.19961996Bacterial Typing Techniques, Base Sequence, DNA Probes/genetics, Genes, Bacterial, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Polymerase Chain Reaction, RNA, Bacterial/*genetics, RNA, Ribosomal, 16S/*genetics, Species Specificity, Streptococcus/*classification/*genetics/isolation & purificationPathogenicity
Metabolism11402009Expression and functional properties of the Streptococcus intermedius surface protein antigen I/II.Petersen FC, Pasco S, Ogier J, Klein JP, Assev S, Scheie AAInfect Immun10.1128/IAI.69.7.4647-4653.20012001Adhesins, Bacterial/genetics/immunology/metabolism/*physiology, Animals, Bacterial Proteins/genetics/immunology/metabolism/*physiology, Cell Line, Collagen/metabolism, Humans, Interleukin-8/metabolism, *Membrane Glycoproteins, Microscopy, Fluorescence, Mutagenesis, Rats, *StreptococcusPathogenicity
18729493Streptococcus mutans and Streptococcus intermedius adhesion to fibronectin films are oppositely influenced by ionic strength.Busscher HJ, van de Belt-Gritter B, Dijkstra RJ, Norde W, van der Mei HCLangmuir10.1021/la80169682008*Bacterial Adhesion, Chemical Phenomena, Chemistry, Physical, *Fibronectins, Microscopy, Atomic Force, Models, Chemical, Osmolar Concentration, Streptococcus intermedius/*cytology, Streptococcus mutans/*cytology, Surface Properties
Metabolism18927421Identification and molecular analysis of betaC-S lyase producing hydrogen sulfide in Streptococcus intermedius.Ito S, Nagamune H, Tamura H, Yoshida YJ Med Microbiol10.1099/jmm.0.2008/001677-02008Base Sequence, Carbon-Sulfur Lyases/*genetics, Cystathionine/metabolism, Cysteine/metabolism, Hydrogen Sulfide/*metabolism, Molecular Sequence Data, Recombinant Proteins/isolation & purification, Streptococcus intermedius/*enzymology/geneticsGenetics
Pathogenicity24610586Effective oral health in infective endocarditis: efficacy of high-street mouthwashes against the viridans group streptococci.Elshibly A, Coulter WA, Millar BC, Prendergast BD, Thornhill M, Irwin C, Goldsmith CE, Moore JEJ Investig Clin Dent10.1111/jicd.120882014Anti-Infective Agents, Local/*pharmacology, Bacteriological Techniques, Cetylpyridinium/pharmacology, Chlorhexidine/analogs & derivatives/pharmacology, Drug Combinations, Endocarditis, Bacterial/*microbiology, Humans, Mouth/*microbiology, Mouthwashes/*pharmacology, Oral Health, Salicylates/pharmacology, Streptococcal Infections/*microbiology, Streptococcus/drug effects, Streptococcus gordonii/drug effects, Streptococcus intermedius/drug effects, Streptococcus mutans/drug effects, Streptococcus oralis/drug effects, Streptococcus pneumoniae/drug effects, Terpenes/pharmacology, Viridans Streptococci/*drug effects

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8923Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20573)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20573
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41341Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15025
51165Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32759)https://www.ccug.se/strain?id=32759
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68370Automatically annotated from API 20STR
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
83850Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10353.1StrainInfo: A central database for resolving microbial strain identifiers
120155Curators of the CIPCollection of Institut Pasteur (CIP 103248)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103248