Strain identifier

BacDive ID: 14731

Type strain: Yes

Species: Streptococcus iniae

Strain Designation: PW

Strain history: CIP <- 1986, ATCC <- G. Pier and S. Madin: strain PW

NCBI tax ID(s): 1346 (species)

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General

@ref: 8926

BacDive-ID: 14731

DSM-Number: 20576

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped

description: Streptococcus iniae PW is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from abscess of amazon fresh water dolphin .

NCBI tax id

  • NCBI tax id: 1346
  • Matching level: species

strain history

@refhistory
8926<- ATCC; ATCC 29178 <- G. B. Pier and S. H. Madin; PW
120119CIP <- 1986, ATCC <- G. Pier and S. Madin: strain PW

doi: 10.13145/bacdive14731.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus iniae
  • full scientific name: Streptococcus iniae Pier and Madin 1976 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus shiloi

@ref: 8926

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus iniae

full scientific name: Streptococcus iniae Pier and Madin 1976

strain designation: PW

type strain: yes

Morphology

cell morphology

  • @ref: 120119
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis abilityincubation period
8926beta11-2 days
1201191

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8926COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8926TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
38975MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
120119CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
8926positivegrowth37mesophilic
38975positivegrowth37mesophilic
48710positivegrowth30-37mesophilic
120119positivegrowth25-37mesophilic
120119nogrowth10psychrophilic
120119nogrowth41thermophilic
120119nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8926microaerophile
48710aerobe
120119facultative anaerobe

halophily

  • @ref: 120119
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 8926
  • murein short key: A11.06
  • type: A3alpha L-Lys-L-Ala3

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose+builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
120119esculin+hydrolysis4853
120119hippurate+hydrolysis606565
120119nitrate-reduction17632
120119nitrite-reduction16301
68381arginine+hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol-builds acid from30911
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381pullulan+builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose+builds acid from6731
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin-
12011915688acetoin-

enzymes

@refvalueactivityec
8926catalase+1.11.1.6
8926cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381beta-glucuronidase+3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
120119oxidase-
120119beta-galactosidase+3.2.1.23
120119alcohol dehydrogenase-1.1.1.1
120119catalase-1.11.1.6
120119gamma-glutamyltransferase-2.3.2.2
120119lysine decarboxylase-4.1.1.18
120119ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120119-+-+-++--+++--++----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120119+---+----++++----+--+/-++/-++++++/--++-+-++-++---------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
8926++++++++--+-+----+++-+-+++-+--+-
8926++++++++--+-+----+++-+-+++-++-+-
8926++++++++-++-+----+++-+-+++-++-+-
8926++++-+++--+-+----+-+----++-+--+-

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitude
8926abscess of amazon fresh water dolphin (Inia geoffrensis)Inia geoffrensisSan FranciscoUSAUSANorth America37.7833-122.417
48710Amazon freshwater dolphin lesion
120119Animal, Amazon fresh water dolphinUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Aquatic mammal

taxonmaps

  • @ref: 69479
  • File name: preview.99_665.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_79;97_244;98_536;99_665&stattab=map
  • Last taxonomy: Streptococcus iniae subclade
  • 16S sequence: DQ303187
  • Sequence Identity:
  • Total samples: 6029
  • soil counts: 47
  • aquatic counts: 134
  • animal counts: 5813
  • plant counts: 35

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
89262Risk group (German classification)
1201192Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus iniae 16S-23S ribosomal RNA spacer region and tRNA-Ala gene, complete sequenceAF048773524ena1346
20218Streptococcus iniae strain ATCC29178 16S ribosomal RNA gene, partial sequenceAF3355721536ena1346
20218Streptococcus iniae strain ATCC 29178 16S ribosomal RNA gene, partial sequenceAY577823534ena1346
20218Streptococcus iniae strain ATCC 29178 16S-23S ribosomal RNA intergenic spacer, partial sequence; and tRNA-Ala (trnA) gene, complete sequenceGU330188511ena1346
8926Streptococcus iniae strain ATCC 29178 16S ribosomal RNA gene, partial sequenceDQ3031871466ena1346

GC content

  • @ref: 8926
  • GC-content: 32.9

External links

@ref: 8926

culture collection no.: CCUG 27303, LMG 14520, DSM 20576, ATCC 29178, CECT 7363, CIP 102508, NCDO 2722

straininfo link

  • @ref: 83849
  • straininfo: 9089

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9228748Restriction fragment length polymorphisms of 16S rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel.Eldar A, Lawhon S, Frelier PF, Assenta L, Simpson BR, Varner PW, Bercovier HFEMS Microbiol Lett10.1111/j.1574-6968.1997.tb12564.x1997DNA, Ribosomal/*analysis, Israel, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/*genetics, Streptococcus/*classification/genetics, United States
Phylogeny16546278Identification of Streptococcus iniae by commercial bacterial identification systems.Roach JC, Levett PN, Lavoie MCJ Microbiol Methods10.1016/j.mimet.2006.02.0122006Animals, Fish Diseases/microbiology, Fishes/microbiology, Polymerase Chain Reaction/*methods, Streptococcus/*classification/genetics/isolation & purificationEnzymology
Enzymology16875393Partial two-dimensional gel electrophoresis (2-DE) maps of Streptococcus iniae ATCC29178 and Lactococcus garvieae KG9408.Shin GW, Palaksha KJ, Yang HH, Shin YS, Kim YR, Lee EY, Oh MJ, Jung TSDis Aquat Organ10.3354/dao0700712006Animals, Bacterial Proteins/*chemistry/*classification, Databases, Protein, Electrophoresis, Gel, Two-Dimensional, Fishes, Lactococcus/chemistry/*genetics/physiology, Proteome/*genetics, Proteomics/methods, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods, Streptococcus/chemistry/*genetics/physiologyPhylogeny
Phylogeny21294750Rapid identification of Streptococcus iniae by specific PCR assay utilizing genetic markers in ITS rDNA.Zhou SM, Fan Y, Zhu XQ, Xie MQ, Li AXJ Fish Dis10.1111/j.1365-2761.2010.01233.x2011Animals, Base Sequence, DNA Primers/genetics, DNA, Bacterial/genetics/*isolation & purification, DNA, Ribosomal Spacer/*genetics/isolation & purification, Fish Diseases/*microbiology, Genetic Markers, Molecular Sequence Data, Polymerase Chain Reaction/*methods, RNA, Ribosomal/genetics/isolation & purification, Sequence Alignment, Streptococcus/classification/genetics/*isolation & purification, Tilapia/*microbiologyEnzymology
22347573Molecular epidemiology of zoonotic streptococcosis/lactococcosis in rainbow trout (Oncorhynchus mykiss) aquaculture in Iran.Haghighi Karsidani S, Soltani M, Nikbakhat-Brojeni G, Ghasemi M, Skall HIran J Microbiol2010
24031591In vitro antibacterial activities of ethanol extract of iranian propolis (EEIP) against fish pathogenic bacteria (Aeromonas hydrophila, Yersinia ruckeri & Streptococcus iniae).Tukmechi A, Ownagh A, Mohebbat ABraz J Microbiol10.1590/S1517-8382201000040000302010
24031866Streptococcus iniae outbreaks in Brazilian Nile tilapia (Oreochromis niloticus L:) farms.Figueiredo HC, Netto LN, Leal CA, Pereira UP, Mian GFBraz J Microbiol10.1590/S1517-838220120002000192012
25097961Streptococcus shiloi, the name for an agent causing septicemic infection in fish, is a junior synonym of Streptococcus iniae.Eldar A, Frelier PF, Assenta L, Varner PW, Lawhon S, Bercovier HInt J Syst Bacteriol10.1099/00207713-45-4-8401995
Pathogenicity34291412The potential risk of antibiotic resistance of Streptococcus iniae in sturgeon cultivation in Sichuan, China.Feng Y, Bai M, Geng Y, Chen D, Huang X, Ouyang P, Guo H, Zuo Z, Huang C, Lai WEnviron Sci Pollut Res Int10.1007/s11356-021-15501-12021Animals, Anti-Bacterial Agents/pharmacology, Aquaculture, China, Drug Resistance, Microbial/genetics, Fishes, Genes, Bacterial, *Streptococcal Infections, *Streptococcus iniaeCultivation
35529822Effects of heat shock protein inducer on Hsp70 gene expression and immune parameters during Streptococcus iniae infection in a Persian sturgeon fry.Baharloei M, Heidari B, Zamani H, Ghafouri H, Hadavi MVet Res Forum10.30466/vrf.2019.115181.27402021

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8926Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20576)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20576
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38975Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14203
48710Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 27303)https://www.ccug.se/strain?id=27303
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83849Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID9089.1StrainInfo: A central database for resolving microbial strain identifiers
120119Curators of the CIPCollection of Institut Pasteur (CIP 102508)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102508