Strain identifier

BacDive ID: 14729

Type strain: Yes

Species: Streptococcus ferus

Strain Designation: 8S1

Strain history: CIP <- 1988, ATCC <- A.L. Coykendall: strain 8S1

NCBI tax ID(s): 1123303 (strain), 1345 (species)

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General

@ref: 8866

BacDive-ID: 14729

DSM-Number: 20646

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, oval-shaped

description: Streptococcus ferus 8S1 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from mouth of wild rat.

NCBI tax id

NCBI tax idMatching level
1123303strain
1345species

strain history

@refhistory
8866<- K.H. Schleifer <- A.L. Coykendall, 8S1
119336CIP <- 1988, ATCC <- A.L. Coykendall: strain 8S1

doi: 10.13145/bacdive14729.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus ferus
  • full scientific name: Streptococcus ferus (ex Coykendall 1977) Coykendall 1983

@ref: 8866

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus ferus

full scientific name: Streptococcus ferus (ex Coykendall 1977) Coykendall 1983 emend. Baele et al. 2003

strain designation: 8S1

type strain: yes

Morphology

cell morphology

  • @ref: 119336
  • gram stain: positive
  • cell shape: oval-shaped
  • motility: no

colony morphology

  • @ref: 8866
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8866PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
34228MEDIUM 38 - for Streptococcus and Gemella HaemolysansyesDistilled water make up to (1000.000 ml);Horse serum (200.000 ml);Glucose (10.000 g);Brain heart infusion agar (52.000 g)
119336CIP Medium 38yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=38
119336CIP Medium 253yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=253

culture temp

@refgrowthtypetemperaturerange
8866positivegrowth37mesophilic
34228positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8866anaerobe
119336facultative anaerobe

spore formation

  • @ref: 119336
  • spore formation: no

murein

  • @ref: 8866
  • murein short key: A11.25
  • type: A3alpha L-Lys-L-Thr-L-Ala

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119336nitrate-reduction17632
119336nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol+builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

@refChebi-IDmetaboliteproduction
11933635581indoleno
6838115688acetoinyes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
119336oxidase-
119336catalase-1.11.1.6
119336urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119336--++-+----++---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119336---------++++----++--++++++++-++---++-++/--+------+/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
8866-+-----++++-+---++-----+++--+---
8866-+-----++++-+---++-------+--+---

Isolation, sampling and environmental information

isolation

@refsample type
8866mouth of wild rat
119336Animal, Wild rat, mouth

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body-Site#Oral cavity and airways#Mouth

taxonmaps

  • @ref: 69479
  • File name: preview.99_1340.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_775;97_903;98_1059;99_1340&stattab=map
  • Last taxonomy: Streptococcus ferus subclade
  • 16S sequence: AY584479
  • Sequence Identity:
  • Total samples: 8418
  • soil counts: 343
  • aquatic counts: 902
  • animal counts: 7058
  • plant counts: 115

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
88661Risk group (German classification)
1193362Risk group (French classification)

Sequence information

16S sequences

  • @ref: 8866
  • description: Streptococcus ferus strain ATCC 33477 16S ribosomal RNA gene, partial sequence
  • accession: AY584479
  • length: 1479
  • database: ena
  • NCBI tax ID: 1345

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus ferus DSM 20646GCA_000372425contigncbi1123303
66792Streptococcus ferus DSM 206461123303.3wgspatric1123303
66792Streptococcus ferus DSM 206462515154024draftimg1123303

GC content

  • @ref: 8866
  • GC-content: 44.2

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno95.936no
flagellatedno96.863no
gram-positiveyes92.112no
anaerobicno94.704no
aerobicno97.868no
halophileyes79.162no
spore-formingno95.843no
thermophileno99.478yes
glucose-utilyes87.849no
glucose-fermentyes87.418no

External links

@ref: 8866

culture collection no.: DSM 20646, ATCC 33477, CIP 103225, NCDO 2721, LMG 16520

straininfo link

  • @ref: 83847
  • straininfo: 14194

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8866Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20646)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20646
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34228Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14999
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83847Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14194.1StrainInfo: A central database for resolving microbial strain identifiers
119336Curators of the CIPCollection of Institut Pasteur (CIP 103225)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103225