Strain identifier

BacDive ID: 14727

Type strain: Yes

Species: Streptococcus equinus

Strain Designation: Hl2B, H12B

Strain history: CIP <- 1986, ATCC <- J.M. Sherman: strain H12B

NCBI tax ID(s): 525379 (strain), 1335 (species)

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General

@ref: 8908

BacDive-ID: 14727

DSM-Number: 20558

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, human pathogen

description: Streptococcus equinus Hl2B is a microaerophile, mesophilic human pathogen that was isolated from Horse feces.

NCBI tax id

NCBI tax idMatching level
1335species
525379strain

strain history

@refhistory
8908<- ATCC <- J.M. Sherman, Hl2B
67770UQM 3541 <-- NCDO 1037 <-- ATCC 9812 <-- J. M. Sherman H12B.
119122CIP <- 1986, ATCC <- J.M. Sherman: strain H12B

doi: 10.13145/bacdive14727.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus equinus
  • full scientific name: Streptococcus equinus Andrewes and Horder 1906 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus bovis

@ref: 8908

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus equinus

full scientific name: Streptococcus equinus Andrewes and Horder 1906

strain designation: Hl2B, H12B

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no95.114
69480100positive
119122nopositivecoccus-shaped

colony morphology

  • @ref: 119122

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8908COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8908TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
40428MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
119122CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
8908positivegrowth37mesophilic
40428positivegrowth37mesophilic
67770positivegrowth37mesophilic
119122positivegrowth30-37mesophilic
119122nogrowth10psychrophilic
119122nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8908microaerophile
119122facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.998

murein

  • @ref: 8908
  • murein short key: A11.29
  • type: A3alpha L-Lys-L-Thr-L-Ser(L-Ala)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119122esculin+hydrolysis4853
119122nitrate-reduction17632
119122nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose-builds acid from17716
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
11912215688acetoin+

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
119122oxidase-
119122beta-galactosidase+3.2.1.23
119122alcohol dehydrogenase-1.1.1.1
119122catalase-1.11.1.6
119122lysine decarboxylase-4.1.1.18
119122ornithine decarboxylase-4.1.1.17
119122urease-3.5.1.5

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119122---------++++--------+++++++--++------+----------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
8908-+--------+-+---++-------+------
8908-+--------+-+---++-------+--+---

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Horse feces

taxonmaps

  • @ref: 69479
  • File name: preview.99_1029.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_100;97_690;98_805;99_1029&stattab=map
  • Last taxonomy: Streptococcus
  • 16S sequence: LC096208
  • Sequence Identity:
  • Total samples: 36809
  • soil counts: 419
  • aquatic counts: 1799
  • animal counts: 33925
  • plant counts: 666

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
8908yesyes2Risk group (German classification)
1191222Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus equinus strain ATCC9812 16S ribosomal RNA gene, partial sequenceAF104116356ena525379
20218Streptococcus equinus NCDO1037T 16S ribosomal RNA gene, complete sequenceAF4297651463ena525379
20218Streptococcus equinus 16S rRNA gene, strain ATCC 9812AJ3016071433ena525379
20218Streptococcus equinus strain ATCC 9812 16S-23S ribosomal RNA intergenic spacer, complete sequenceAY347547284ena525379
20218Streptococcus equinus gene for 16S rRNA, partial sequence, strain: NBRC 12553AB6802951467ena1335
20218S.equinus 16S rRNAX583181334ena1335
67770Streptococcus equinus gene for 16S ribosomal RNA, partial sequence, strain: JCM 7879LC0962081498ena1335

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus equinus NCTC12969GCA_900459295contigncbi1335
66792Streptococcus equinus ATCC 9812GCA_000187265scaffoldncbi525379
66792Streptococcus equinus ATCC 9812525379.3wgspatric525379
66792Streptococcus equinus strain NCTC129691335.79wgspatric1335
66792Streptococcus equinus ATCC 9812649990006draftimg525379

GC content

@refGC-contentmethod
890837.6
6777037.6thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.674no
flagellatedno96.064no
gram-positiveyes94.59no
anaerobicno88.615yes
aerobicno98.37yes
halophileyes73.892no
spore-formingno95.199no
glucose-utilyes89.928no
thermophileno99.204yes
glucose-fermentyes81.449no

External links

@ref: 8908

culture collection no.: DSM 20558, ATCC 9812, NCDO 1037, CCUG 27302, IFO 12553, JCM 7879, BCRC 12578, CECT 973, CGMCC 1.2506, CIP 102504, HAMBI 1572, LMG 14897, NBRC 12553, NCIMB 701037, NCTC 12969

straininfo link

  • @ref: 83845
  • straininfo: 8748

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8908Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20558)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20558
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40428Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14197
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83845Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8748.1StrainInfo: A central database for resolving microbial strain identifiers
119122Curators of the CIPCollection of Institut Pasteur (CIP 102504)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102504