Strain identifier
BacDive ID: 14719
Type strain:
Species: Streptococcus equinus
Strain Designation: Pearl 11
Strain history: CIP <- 1986, NCFB <- P.M.F. Shattock, Reading, UK: strain Pearl 11
NCBI tax ID(s): 1210006 (strain), 1335 (species)
General
@ref: 8849
BacDive-ID: 14719
DSM-Number: 20480
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, human pathogen
description: Streptococcus equinus Pearl 11 is a microaerophile, mesophilic human pathogen that was isolated from cow dung.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1210006 | strain |
1335 | species |
strain history
@ref | history |
---|---|
8849 | <- NCDO <- P.M.F. Shattock, Pearl 11 |
67770 | DSM 20480 <-- NCDO 597 <-- P. M. F. Shattock strain Pearl 11. |
119123 | CIP <- 1986, NCFB <- P.M.F. Shattock, Reading, UK: strain Pearl 11 |
doi: 10.13145/bacdive14719.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Streptococcaceae
- genus: Streptococcus
- species: Streptococcus equinus
- full scientific name: Streptococcus equinus Andrewes and Horder 1906 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Streptococcus bovis
@ref: 8849
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Streptococcaceae
genus: Streptococcus
species: Streptococcus equinus
full scientific name: Streptococcus equinus Andrewes and Horder 1906
strain designation: Pearl 11
type strain: no
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 91.93 | ||
69480 | 100 | positive | ||
119123 | no | positive | coccus-shaped |
colony morphology
@ref | type of hemolysis | hemolysis ability | incubation period |
---|---|---|---|
8849 | alpha | 1 | 1-2 days |
119123 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8849 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
8849 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
38102 | MEDIUM 6 - Columbia agar with 10 % horse blood | yes | Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml) | |
119123 | CIP Medium 6 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8849 | positive | growth | 37 | mesophilic |
38102 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
119123 | positive | growth | 25-45 | |
119123 | no | growth | 10 | psychrophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
8849 | microaerophile |
119123 | facultative anaerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.991 |
halophily
- @ref: 119123
- salt: NaCl
- growth: no
- tested relation: growth
- concentration: 6.5 %
murein
- @ref: 8849
- murein short key: A11.29
- type: A3alpha L-Lys-L-Thr-L-Ser(L-Ala)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
119123 | 4853 | esculin | + | hydrolysis |
119123 | 606565 | hippurate | - | hydrolysis |
119123 | 17632 | nitrate | - | reduction |
119123 | 16301 | nitrite | - | reduction |
119123 | 17632 | nitrate | + | respiration |
68381 | 29016 | arginine | - | hydrolysis |
68381 | 16899 | D-mannitol | - | builds acid from |
68381 | 30911 | sorbitol | - | builds acid from |
68381 | 17716 | lactose | + | builds acid from |
68381 | 27082 | trehalose | - | builds acid from |
68381 | 16634 | raffinose | + | builds acid from |
68381 | 17992 | sucrose | + | builds acid from |
68381 | 30849 | L-arabinose | - | builds acid from |
68381 | 18333 | D-arabitol | - | builds acid from |
68381 | 40585 | alpha-cyclodextrin | - | builds acid from |
68381 | 606565 | hippurate | - | hydrolysis |
68381 | 28087 | glycogen | + | builds acid from |
68381 | 17306 | maltose | + | builds acid from |
68381 | 28053 | melibiose | + | builds acid from |
68381 | 6731 | melezitose | - | builds acid from |
68381 | 320055 | methyl beta-D-glucopyranoside | + | builds acid from |
68381 | 16443 | D-tagatose | - | builds acid from |
68381 | 16199 | urea | - | hydrolysis |
antibiotic resistance
- @ref: 119123
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- production: yes
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test |
---|---|---|---|
68381 | 15688 | acetoin | + |
119123 | 15688 | acetoin | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
8849 | catalase | - | 1.11.1.6 |
8849 | cytochrome-c oxidase | - | 1.9.3.1 |
68381 | urease | - | 3.5.1.5 |
68381 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68381 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68381 | beta-galactosidase | - | 3.2.1.23 |
68381 | Alanyl-Phenylalanyl-Proline arylamidase | + | |
68381 | alkaline phosphatase | - | 3.1.3.1 |
68381 | alpha-galactosidase | + | 3.2.1.22 |
68381 | beta-glucuronidase | - | 3.2.1.31 |
68381 | beta-glucosidase | + | 3.2.1.21 |
68381 | arginine dihydrolase | - | 3.5.3.6 |
119123 | oxidase | - | |
119123 | beta-galactosidase | + | 3.2.1.23 |
119123 | alcohol dehydrogenase | - | 1.1.1.1 |
119123 | catalase | - | 1.11.1.6 |
119123 | gamma-glutamyltransferase | - | 2.3.2.2 |
119123 | lysine decarboxylase | - | 4.1.1.18 |
119123 | ornithine decarboxylase | - | 4.1.1.17 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119123 | - | - | + | + | - | + | - | - | - | - | + | - | + | - | - | + | + | - | - | - |
API rID32STR
@ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8849 | - | + | - | - | + | - | - | - | - | + | - | + | + | - | - | - | + | + | - | - | - | - | - | + | + | + | + | - | + | - | + | - |
8849 | - | + | - | - | + | - | +/- | - | - | + | - | + | + | - | - | - | + | + | - | - | - | - | - | + | - | + | + | - | + | - | +/- | - |
8849 | - | + | - | - | + | - | - | - | - | + | - | + | + | - | - | - | + | + | - | - | - | + | - | + | + | + | + | - | + | - | - | - |
8849 | - | + | - | - | + | - | - | - | - | + | - | + | + | - | - | - | + | + | - | - | - | + | - | + | + | + | + | - | + | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
8849 | cow dung |
67770 | Cow feces |
119123 | Animal, Cow, dung |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Bovinae (Cow, Cattle) |
#Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
taxonmaps
- @ref: 69479
- File name: preview.99_1029.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_100;97_690;98_805;99_1029&stattab=map
- Last taxonomy: Streptococcus
- 16S sequence: LC145574
- Sequence Identity:
- Total samples: 36809
- soil counts: 419
- aquatic counts: 1799
- animal counts: 33925
- plant counts: 666
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
8849 | yes | yes | 2 | Risk group (German classification) |
119123 | 2 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptococcus bovis strain ATCC 33317 16S-23S ribosomal RNA intergenic spacer, complete sequence | AY347544 | 271 | ena | 1210006 |
20218 | Streptococcus bovis 16S ribosomal RNA | M58835 | 1551 | ena | 1335 |
20218 | S.bovis 16S rRNA | X58317 | 1334 | ena | 1335 |
8849 | Streptococcus bovis DNA for 16S rRNA, strain ATCC 33317, NCDO 597 | AB002482 | 1457 | ena | 1210006 |
67770 | Streptococcus equinus gene for 16S ribosomal RNA, partial sequence, strain: JCM 5802 | LC145574 | 1501 | ena | 1335 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Streptococcus equinus ATCC 33317 | GCA_000747195 | contig | ncbi | 1210006 |
66792 | Streptococcus equinus ATCC 33317 | 1210006.5 | wgs | patric | 1210006 |
66792 | Streptococcus bovis ATCC 33317 | 2600255054 | draft | img | 1210006 |
GC content
@ref | GC-content | method |
---|---|---|
8849 | 37.7 | |
67770 | 37.6 | thermal denaturation, midpoint method (Tm) |
67770 | 37.7 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 93.305 | no |
gram-positive | yes | 94.14 | no |
anaerobic | no | 88.399 | yes |
aerobic | no | 98.319 | yes |
halophile | yes | 79.667 | no |
spore-forming | no | 95.188 | no |
glucose-util | yes | 89.317 | no |
flagellated | no | 96.052 | no |
thermophile | no | 99.417 | yes |
glucose-ferment | yes | 76.394 | no |
External links
@ref: 8849
culture collection no.: DSM 20480, ATCC 33317, NCDO 597, NCTC 8177, WDCM 00133, JCM 5802, ACM 3539, CCUG 17828, CCUG 34832, CECT 213, CECT 974, CGMCC 1.2502, CIP 102302, JCM 7877, LMG 8518, NCIMB 700597
straininfo link
- @ref: 83837
- straininfo: 92503
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 11283085 | 16S ribosomal DNA sequence analysis distinguishes biotypes of Streptococcus bovis: Streptococcus bovis Biotype II/2 is a separate genospecies and the predominant clinical isolate in adult males. | Clarridge JE 3rd, Attorri SM, Zhang Q, Bartell J | J Clin Microbiol | 10.1128/JCM.39.4.1549-1552.2001 | 2001 | Adult, *Bacterial Typing Techniques, Child, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, *Genes, rRNA, Genotype, Humans, Male, Molecular Sequence Data, Phenotype, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, Streptococcal Infections/*microbiology, Streptococcus bovis/*classification/genetics/isolation & purification | Genetics |
Genetics | 25301652 | Draft Genome Sequences of Streptococcus bovis Strains ATCC 33317 and JB1. | Benahmed FH, Gopinath GR, Harbottle H, Cotta MA, Luo Y, Henderson C, Teri P, Soppet D, Rasmussen M, Whitehead TR, Davidson M | Genome Announc | 10.1128/genomeA.01012-14 | 2014 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
8849 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20480) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-20480 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
38102 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/13973 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68381 | Automatically annotated from API rID32STR | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
83837 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID92503.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119123 | Curators of the CIP | Collection of Institut Pasteur (CIP 102302) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102302 |