Strain identifier

BacDive ID: 147

Type strain: No

Species: Actinobaculum massiliense

Strain history: <- CCUG <- CIP <- C. Bibard

NCBI tax ID(s): 202789 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 7917

BacDive-ID: 147

DSM-Number: 19118

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Actinobaculum massiliense DSM 19118 is an anaerobe, mesophilic bacterium that was isolated from human urine, 81-yr-old woman with cystitis.

NCBI tax id

  • NCBI tax id: 202789
  • Matching level: species

strain history

  • @ref: 7917
  • history: <- CCUG <- CIP <- C. Bibard

doi: 10.13145/bacdive147.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Actinobaculum
  • species: Actinobaculum massiliense
  • full scientific name: Actinobaculum massiliense corrig. Greub and Raoult 2002

  • synonyms

    • @ref: 20215
    • synonym: Actinobaculum massiliae

@ref: 7917

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Actinobaculum

species: Actinobaculum massiliense

full scientific name: Actinobaculum massiliense Greub and Raoult 2006

type strain: no

Morphology

colony morphology

  • @ref: 57854
  • incubation period: 2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7917PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
7917CHOCOLATE AGAR (DSMZ Medium 429b)yeshttps://mediadive.dsmz.de/medium/429bName: CHOCOLATE AGAR (DSMZ Medium 429b) Composition: Horse blood 100.0 g/l Sheep blood 100.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
7917positivegrowth37mesophilic
57854positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7917anaerobe
57854anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase+3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
7917-----+++---+/------++++++++++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
7917human urine, 81-yr-old woman with cystitisMarseilleFranceFRAEurope
57854Human urine,81-yr-old woman,cystitisMarseilleFranceFRAEurope2001

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Human#Female
#Host Body Product#Fluids#Urine

taxonmaps

  • @ref: 69479
  • File name: preview.99_1698.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_247;96_965;97_1124;98_1340;99_1698&stattab=map
  • Last taxonomy: Actinobaculum massiliense subclade
  • 16S sequence: AF487679
  • Sequence Identity:
  • Total samples: 1977
  • soil counts: 31
  • aquatic counts: 21
  • animal counts: 1924
  • plant counts: 1

Safety information

risk assessment

  • @ref: 7917
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Actinobaculum massiliense strain CIP 107404 16S ribosomal RNA gene, partial sequence
  • accession: AF487679
  • length: 1504
  • database: ena
  • NCBI tax ID: 202789

External links

@ref: 7917

culture collection no.: DSM 19118, CCUG 47753, CIP 107404

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny25406238Dissection of the genus Actinobaculum: Reclassification of Actinobaculum schaalii Lawson et al. 1997 and Actinobaculum urinale Hall et al. 2003 as Actinotignum schaalii gen. nov., comb. nov. and Actinotignum urinale comb. nov., description of Actinotignum sanguinis sp. nov. and emended descriptions of the genus Actinobaculum and Actinobaculum suis; and re-examination of the culture deposited as Actinobaculum massiliense CCUG 47753T ( = DSM 19118T), revealing that it does not represent a strain of this species.Yassin AF, Sproer C, Pukall R, Sylvester M, Siering C, Schumann PInt J Syst Evol Microbiol10.1099/ijs.0.069294-02014Actinomycetaceae/*classification/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny25479952The status of the species Actinobaculum massiliense (Greub and Raoult 2006). Request for an Opinion.Yassin AF, Sproer C, Pukall R, Schumann PInt J Syst Evol Microbiol10.1099/ijs.0.0000112014Actinomycetaceae/*classification, *Phylogeny, RNA, Ribosomal, 16S/genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7917Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19118)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19118
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
57854Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47753)https://www.ccug.se/strain?id=47753
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta