Strain identifier

BacDive ID: 14699

Type strain: No

Species: Lactococcus lactis

Strain Designation: 117

Strain history: CIP <- 1987, NCFB <- 1984, DSM <- ATCC <- K. Kitahara: strain 117, Lactobacillus xylosus

NCBI tax ID(s): 1358 (species)

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General

@ref: 8605

BacDive-ID: 14699

DSM-Number: 20175

keywords: genome sequence, 16S sequence, Bacteria, human pathogen

description: Lactococcus lactis 117 is a human pathogen that was isolated from "Anchu" mash.

NCBI tax id

  • NCBI tax id: 1358
  • Matching level: species

strain history

@refhistory
8605<- ATCC <- K. Kitahara, 117 (Lactobacillus xylosus)
67770IAM 10070 <-- K. Kitahara.
67770ATCC 15577 <-- K. Kitahara 117.
120527CIP <- 1987, NCFB <- 1984, DSM <- ATCC <- K. Kitahara: strain 117, Lactobacillus xylosus

doi: 10.13145/bacdive14699.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Lactococcus
  • species: Lactococcus lactis
  • full scientific name: Lactococcus lactis (Lister 1873) Schleifer et al. 1986

  • synonyms

    @refsynonym
    20215Streptococcus lactis
    20215Bacterium lactis
    20215Lactobacillus xylosus
    20215Streptococcus lactis subsp. diacetilactis

@ref: 8605

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Lactococcus

species: Lactococcus lactis

full scientific name: Lactococcus lactis (Lister 1873) Schleifer et al. 1986

strain designation: 117

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no92
6948093.898positive

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8605TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
38748MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
120527CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
8605positivegrowth30
38748positivegrowth30
67770positivegrowth30

Physiology and metabolism

murein

  • @ref: 8605
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

observation

  • @ref: 67770
  • observation: quinones: MK-9, MK-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838129016arginine+hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin+builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
8605++---+/-----+-+--++--------+--+-+/--

Isolation, sampling and environmental information

isolation

@refsample type
8605"Anchu" mash
67770Anchu mash
120527Environment, Anchu mash

taxonmaps

  • @ref: 69479
  • File name: preview.99_170.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_58;96_124;97_132;98_149;99_170&stattab=map
  • Last taxonomy: Lactococcus
  • 16S sequence: LC311732
  • Sequence Identity:
  • Total samples: 157686
  • soil counts: 7135
  • aquatic counts: 13841
  • animal counts: 132033
  • plant counts: 4677

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
8605yes, in single cases1Risk group (German classification)
1205271Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
67770Lactococcus lactis subsp. lactis gene for 16S ribosomal RNA, partial sequence, strain: JCM 1158LC1535491485nuccore1360
67770Lactococcus lactis subsp. lactis gene for 16S ribosomal RNA, partial sequence, strain: JCM 20312LC3117321483nuccore1360

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactococcus lactis subsp. lactis LMG 7760GCA_001437565scaffoldncbi1360
66792Lactococcus lactis subsp. lactis strain LMG 77601360.94wgspatric1360
66792Lactococcus lactis lactis LMG 77602663763414draftimg1360

GC content

@refGC-contentmethod
860539.4
6777039.4thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes93.898no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no90.77no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no95.309no
69480spore-formingspore-formingAbility to form endo- or exosporesno85.606no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno98.5yes
69480flagellatedmotile2+Ability to perform flagellated movementno92no

External links

@ref: 8605

culture collection no.: DSM 20175, ATCC 15577, CCUG 21962, NRRL B-4449, LMG 7760, JCM 1158, CECT 984, NCFB 2738, NRIC 1074, JCM 20312, IAM 10070, CIP 102975, NCDO 2738

straininfo link

  • @ref: 83818
  • straininfo: 125702

literature

  • topic: Metabolism
  • Pubmed-ID: 11282589
  • title: Genetic evidence for a defective xylan degradation pathway in Lactococcus lactis.
  • authors: Erlandson KA, Delamarre SC, Batt CA
  • journal: Appl Environ Microbiol
  • DOI: 10.1128/AEM.67.4.1445-1452.2001
  • year: 2001
  • mesh: Amino Acid Sequence, Bacterial Proteins, Biodegradation, Environmental, Carbohydrate Epimerases/chemistry/*genetics/metabolism, Genes, Bacterial, Glycosides/metabolism, Lactococcus lactis/*genetics/metabolism, Molecular Sequence Data, Phosphotransferases (Alcohol Group Acceptor)/genetics, Sequence Alignment, Sequence Analysis, DNA, Transcription, Genetic, Xylans/genetics/*metabolism, Xylosidases/genetics/*metabolism
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
8605Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20175)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20175
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
38748Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14720
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
83818Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID125702.1StrainInfo: A central database for resolving microbial strain identifiers
120527Curators of the CIPCollection of Institut Pasteur (CIP 102975)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102975