Strain identifier
BacDive ID: 14691
Type strain:
Species: Lactococcus cremoris
Strain Designation: HP
Strain history: CIP <- 1986, NCFB, Streptococcus cremoris <- H.R. Whitehead: strain HP
NCBI tax ID(s): 1348655 (strain), 1359 (species)
General
@ref: 8499
BacDive-ID: 14691
DSM-Number: 20069
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped, human pathogen
description: Lactococcus cremoris HP is a microaerophile, mesophilic, Gram-positive human pathogen of the family Streptococcaceae.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1359 | species |
1348655 | strain |
strain history
@ref | history |
---|---|
8499 | <- ATCC <- NCDO <- NIRD <- Whitehead, HP |
67770 | CIP 102301 <-- NCFB 607 <-- H. R. Whitehead strain HP. |
118783 | CIP <- 1986, NCFB, Streptococcus cremoris <- H.R. Whitehead: strain HP |
doi: 10.13145/bacdive14691.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Streptococcaceae
- genus: Lactococcus
- species: Lactococcus cremoris
- full scientific name: Lactococcus cremoris (Orla-Jensen 1919) Li et al. 2021
synonyms
@ref synonym 20215 Streptococcus cremoris 20215 Lactococcus lactis subsp. cremoris 20215 Streptococcus lactis subsp. cremoris
@ref: 8499
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Streptococcaceae
genus: Lactococcus
species: Lactococcus cremoris subsp. cremoris
full scientific name: Lactococcus cremoris subsp. cremoris (Orla-Jensen 1919) Li et al. 2021
strain designation: HP
type strain: yes
Morphology
cell morphology
- @ref: 118783
- gram stain: positive
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8499 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
42090 | MEDIUM 40- for Lactobacillus and Leuconostoc | yes | Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g) | |
118783 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
118783 | CIP Medium 40 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8499 | positive | growth | 30 | mesophilic |
42090 | positive | growth | 30 | mesophilic |
47521 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
118783 | positive | growth | 15-30 | |
118783 | no | growth | 10 | psychrophilic |
118783 | no | growth | 37 | mesophilic |
118783 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
47521 | microaerophile |
118783 | facultative anaerobe |
murein
- @ref: 8499
- murein short key: A11.31
- type: A4alpha L-Lys-D-Asp
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
118783 | 17632 | nitrate | - | reduction |
118783 | 16301 | nitrite | - | reduction |
118783 | 17632 | nitrate | + | respiration |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | + | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | - | hydrolysis |
68379 | 16988 | D-ribose | + | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17716 | lactose | + | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
68381 | 29016 | arginine | - | hydrolysis |
68381 | 16988 | D-ribose | - | builds acid from |
68381 | 16899 | D-mannitol | - | builds acid from |
68381 | 30911 | sorbitol | - | builds acid from |
68381 | 17716 | lactose | + | builds acid from |
68381 | 27082 | trehalose | - | builds acid from |
68381 | 16634 | raffinose | - | builds acid from |
68381 | 17992 | sucrose | - | builds acid from |
68381 | 30849 | L-arabinose | - | builds acid from |
68381 | 18333 | D-arabitol | - | builds acid from |
68381 | 40585 | alpha-cyclodextrin | - | builds acid from |
68381 | 28087 | glycogen | - | builds acid from |
68381 | 27941 | pullulan | - | builds acid from |
68381 | 17306 | maltose | - | builds acid from |
68381 | 28053 | melibiose | - | builds acid from |
68381 | 6731 | melezitose | - | builds acid from |
68381 | 320055 | methyl beta-D-glucopyranoside | - | builds acid from |
68381 | 16443 | D-tagatose | - | builds acid from |
68381 | 16199 | urea | - | hydrolysis |
antibiotic resistance
- @ref: 118783
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: yes
- is resistant: no
metabolite production
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- production: yes
metabolite tests
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: +
enzymes
@ref | value | activity | ec |
---|---|---|---|
68381 | urease | - | 3.5.1.5 |
68381 | beta-mannosidase | - | 3.2.1.25 |
68381 | glycyl tryptophan arylamidase | - | |
68381 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68381 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68381 | beta-galactosidase | - | 3.2.1.23 |
68381 | Alanyl-Phenylalanyl-Proline arylamidase | + | |
68381 | alpha-galactosidase | - | 3.2.1.22 |
68381 | beta-glucuronidase | - | 3.2.1.31 |
68381 | arginine dihydrolase | - | 3.5.3.6 |
68379 | gelatinase | - | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | + | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | - | 3.2.1.20 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | - | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
68379 | pyrazinamidase | + | 3.5.1.B15 |
118783 | oxidase | - | |
118783 | alcohol dehydrogenase | - | 1.1.1.1 |
118783 | catalase | - | 1.11.1.6 |
118783 | lysine decarboxylase | - | 4.1.1.18 |
118783 | ornithine decarboxylase | - | 4.1.1.17 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | Control | RIB | XYL | MAN | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
47521 | - | + | + | - | - | - | - | - | + | - | - | - | + | - | - | + | - | - |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
118783 | - | - | + | + | - | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - |
API rID32STR
@ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8499 | - | + | + | - | - | - | - | - | - | + | - | - | - | - | - | - | + | + | - | - | - | - | + | - | - | - | - | - | - | - | - | - |
8499 | - | + | +/- | - | - | + | - | - | - | + | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
8499 | - | + | + | - | - | +/- | - | - | - | + | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
8499 | - | +/- | - | - | - | - | - | - | - | + | - | - | - | - | - | - | + | + | - | - | - | - | + | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | country | origin.country | continent | sample type |
---|---|---|---|---|
42090 | New zealand | NZL | Australia and Oceania | |
47521 | Cheese starter culture | |||
67770 | Raw milk | |||
118783 | New Zealand | NZL | Australia and Oceania |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Food production | #Dairy product |
#Engineered | #Food production | #Starter culture |
taxonmaps
- @ref: 69479
- File name: preview.99_170.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_58;96_124;97_132;98_149;99_170&stattab=map
- Last taxonomy: Lactococcus
- 16S sequence: LC483568
- Sequence Identity:
- Total samples: 157686
- soil counts: 7135
- aquatic counts: 13841
- animal counts: 132033
- plant counts: 4677
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
8499 | yes, in single cases | yes, in single cases | 1 | Risk group (German classification) |
118783 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Lactococcus lactis subsp. lactis gene for 16S rRNA, partial sequence, strain: YIT 2008 (= ATCC 19435) | AB008215 | 1540 | ena | 1360 |
20218 | Streptococcus cremoris 16S ribosomal RNA | M58836 | 1496 | ena | 1359 |
20218 | Lactococcus lactis subsp. cremoris strain NIZO2418T 16S ribosomal RNA gene, partial sequence | EU091472 | 519 | ena | 1359 |
20218 | Lactococcus lactis subsp. cremoris gene for 16S rRNA, partial sequence, strain: NBRC 100676 | AB681219 | 1466 | ena | 1359 |
20218 | Lactococcus lactis subsp. cremoris gene for 16S rRNA, partial sequence, strain: NCDO 607T | AB100802 | 1499 | ena | 1359 |
67770 | Lactococcus lactis subsp. cremoris gene for 16S rRNA, partial sequence, strain: YIT 2007 (= ATCC 19257) | AB008214 | 1540 | ena | 1359 |
67770 | Lactococcus lactis subsp. cremoris gene for 16S ribosomal RNA, partial sequence | LC483568 | 1485 | ena | 1359 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Lactococcus cremoris ATCC 19257 | GCA_002441765 | scaffold | ncbi | 1359 |
66792 | Lactococcus cremoris HP | GCA_001622215 | contig | ncbi | 1359 |
66792 | Lactococcus lactis subsp. cremoris NBRC 100676 | 1348655.3 | wgs | patric | 1348655 |
66792 | Lactococcus lactis subsp. cremoris strain ATCC 19257 | 1359.227 | wgs | patric | 1359 |
66792 | Lactococcus lactis subsp. cremoris strain ATCC 19257 | 1359.117 | wgs | patric | 1359 |
66792 | Lactococcus lactis subsp. cremoris strain LMG6897 | 1359.37 | wgs | patric | 1359 |
66792 | Lactococcus lactis ATCC 19257 | 2903255457 | draft | img | 1358 |
66792 | Lactococcus lactis cremoris NBRC 100676 | 2731957663 | draft | img | 1348655 |
66792 | Lactococcus cremoris LMG6897 | 2740892534 | draft | img | 1359 |
67770 | Lactococcus cremoris subsp. cremoris NBRC 100676 | GCA_001591705 | contig | ncbi | 1348655 |
67770 | Lactococcus cremoris LMG6897 | GCA_001622295 | contig | ncbi | 1359 |
67770 | Lactococcus cremoris ATCC 19257 | GCA_004354515 | scaffold | ncbi | 1359 |
66792 | Lactococcus cremoris subsp. cremoris HP | GCA_000534815 | contig | ncbi | 1295827 |
GC content
@ref | GC-content | method |
---|---|---|
8499 | 35.4 | |
67770 | 34.8-35.6 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 95.845 | no |
gram-positive | yes | 94.11 | no |
anaerobic | no | 94.888 | no |
halophile | yes | 73.802 | no |
spore-forming | no | 93.622 | no |
thermophile | no | 99.623 | yes |
glucose-util | yes | 90.001 | no |
aerobic | no | 97.193 | yes |
flagellated | no | 96.999 | no |
glucose-ferment | yes | 89.268 | no |
External links
@ref: 8499
culture collection no.: DSM 20069, ATCC 19257, NCDO 607, NCIB 8662, CCUG 21953, LMG 6897, IMET 10707, JCM 16167, BCRC 12586, CECT 7100, CIP 102301, CNCTC 6910, HAMBI 1588, NBRC 100676, NCFB 607, NCIMB 8662
straininfo link
- @ref: 83814
- straininfo: 92452
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 15599522 | Quantification by real-time PCR of Lactococcus lactis subsp. cremoris in milk fermented by a mixed culture. | Grattepanche F, Lacroix C, Audet P, Lapointe G | Appl Microbiol Biotechnol | 10.1007/s00253-004-1705-4 | 2004 | Animals, Base Sequence, Colony Count, Microbial/*methods, DNA Primers/genetics, DNA, Bacterial/genetics, Drug Resistance, Bacterial, Fermentation, Food Microbiology, Food Technology, Lactococcus lactis/classification/drug effects/*genetics/*isolation & purification, Milk/*microbiology, Nisin/*analogs & derivatives/pharmacology, Polymerase Chain Reaction/*methods, Species Specificity | Biotechnology |
Phylogeny | 33650946 | Elevation of Lactococcus lactis subsp. cremoris to the species level as Lactococcus cremoris sp. nov. and transfer of Lactococcus lactis subsp. tructae to Lactococcus cremoris as Lactococcus cremoris subsp. tructae comb. nov. | Li TT, Tian WL, Gu CT | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004727 | 2021 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
8499 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20069) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-20069 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
42090 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/13972 | ||||
47521 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 21953) | https://www.ccug.se/strain?id=21953 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68379 | Automatically annotated from API Coryne | |||||
68381 | Automatically annotated from API rID32STR | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
83814 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID92452.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
118783 | Curators of the CIP | Collection of Institut Pasteur (CIP 102301) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102301 |