Strain identifier

BacDive ID: 14660

Type strain: Yes

Species: Mammaliicoccus vitulinus

Strain Designation: DD756

Strain history: CIP <- 1996, N. El Solh, Inst. Pasteur, Paris, France, Staphylococcus vitulus <- ATCC <- W.E. Kloos: strain DD756 <- J.A. Webster

NCBI tax ID(s): 71237 (species)

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General

@ref: 5933

BacDive-ID: 14660

DSM-Number: 15615

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, human pathogen

description: Mammaliicoccus vitulinus DD756 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from ground lamb.

NCBI tax id

  • NCBI tax id: 71237
  • Matching level: species

strain history

@refhistory
5933<- ATCC <- W. E. Kloos; DD756 <- J. A. Webster
123881CIP <- 1996, N. El Solh, Inst. Pasteur, Paris, France, Staphylococcus vitulus <- ATCC <- W.E. Kloos: strain DD756 <- J.A. Webster

doi: 10.13145/bacdive14660.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Mammaliicoccus
  • species: Mammaliicoccus vitulinus
  • full scientific name: Mammaliicoccus vitulinus (Webster et al. 1994) Madhaiyan et al. 2020

  • synonyms

    @refsynonym
    20215Staphylococcus vitulus
    20215Staphylococcus pulvereri
    20215Staphylococcus vitulinus

@ref: 5933

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Mammaliicoccus

species: Mammaliicoccus vitulinus

full scientific name: Mammaliicoccus vitulinus (Webster et al. 1994) Madhaiyan et al. 2020

strain designation: DD756

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
123881positivecoccus-shapedno

colony morphology

  • @ref: 123881
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5933TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
42178MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123881CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
5933positivegrowth37mesophilic
42178positivegrowth37mesophilic
51648positivegrowth25-37mesophilic
123881positivegrowth10-41
123881nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
51648aerobe
123881facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.794

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123881hippurate+hydrolysis606565
123881nitrate+reduction17632
123881nitrite-reduction16301
68375urea-hydrolysis16199
68375arginine-hydrolysis29016
68375ornithine-degradation18257
68375esculin-hydrolysis4853
68375D-glucose+fermentation17634
68375D-fructose+fermentation15824
68375D-mannose-fermentation16024
68375maltose-fermentation17306
68375lactose-fermentation17716
68375trehalose-fermentation27082
68375raffinose-fermentation16634
68375D-ribose-fermentation16988
68375cellobiose-fermentation17057
68375nitrate+reduction17632
68375sucrose+fermentation17992
68375N-acetylglucosamine-fermentation59640
68375turanose-fermentation32528
68375arabinose-fermentation22599

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is resistant: yes
  • resistance conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinno
12388135581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
6837515688acetoin-
12388115688acetoin+
12388117234glucose+

enzymes

@refvalueactivityec
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase-3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5
123881oxidase+
123881beta-galactosidase-3.2.1.23
123881alcohol dehydrogenase-1.1.1.1
123881gelatinase+/-
123881amylase-
123881DNase-
123881caseinase+3.4.21.50
123881catalase+1.11.1.6
123881coagulase-
123881tween esterase-
123881gamma-glutamyltransferase-2.3.2.2
123881lecithinase-
123881lipase-
123881lysine decarboxylase-4.1.1.18
123881ornithine decarboxylase-4.1.1.17
123881protease+
123881urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    51648C14:00.414
    51648C16:00.616
    51648C13:0 ANTEISO0.712.701
    51648C13:0 iso2.312.612
    51648C14:0 ISO213.618
    51648C15:0 ANTEISO43.214.711
    51648C15:0 ISO35.914.621
    51648C16:0 iso0.415.626
    51648C16:1 ω11c115.757
    51648C16:1 ω7c alcohol0.715.386
    51648C17:0 anteiso3.916.722
    51648C17:0 iso2.216.629
    51648C17:1 ISO I/C16:0 DMA1.716.481
    51648C17:1 ω10c ISO3.116.387
    51648C18:2 ω6,9c/C18:0 ANTE117.724
    51648unknown 15.6650.915.665
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123881-+++-+----++---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123881----------+++/-----+/-------------+-------+/-----------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
5933----++----+/----+-----++----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123881+++++--+--+-----++-+-++---+-+--+-++++----+-------+-+-------++------------+-+--+++-----+--++++++---+

Isolation, sampling and environmental information

isolation

@refsample type
5933ground lamb
51648Ground lamb
123881Ground lamb

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Meat
#Host#Mammals#Caprinae (Sheep/Goat)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4196.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_96;98_105;99_4196&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: AB009946
  • Sequence Identity:
  • Total samples: 998
  • soil counts: 25
  • aquatic counts: 30
  • animal counts: 892
  • plant counts: 51

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
5933yes, in single cases1Risk group (German classification)
1238811Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus vitulinus rrn gene for 16S ribosomal RNAAB0099461497ena71237
20218Staphylococcus vitulinus strain ATCC 51145 16S ribosomal RNA gene, partial sequenceAY688104465ena71237

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mammaliicoccus vitulinus DSM 15615GCA_002902265scaffoldncbi71237
66792Mammaliicoccus vitulinus strain FDAARGOS_120771237.31completepatric71237
66792Staphylococcus vitulinus strain DSM 1561571237.13wgspatric71237
66792Mammaliicoccus vitulinus DSM 156152917439556draftimg71237

GC content

  • @ref: 5933
  • GC-content: 34

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes54no
gram-positiveyes92.552no
anaerobicno99.643yes
halophileyes95.053no
spore-formingno89.383no
glucose-utilyes86.611no
thermophileno99.743yes
flagellatedno92.693no
aerobicyes94.686no
motileno89.677no
glucose-fermentyes77.983yes

External links

@ref: 5933

culture collection no.: CCUG 33636, DSM 15615, ATCC 51145, CIP 104850

straininfo link

  • @ref: 83786
  • straininfo: 42827

literature

  • topic: Phylogeny
  • Pubmed-ID: 7520736
  • title: Identification of the Staphylococcus sciuri species group with EcoRI fragments containing rRNA sequences and description of Staphylococcus vitulus sp. nov.
  • authors: Webster JA, Bannerman TL, Hubner RJ, Ballard DN, Cole EM, Bruce JL, Fiedler F, Schubert K, Kloos WE
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-44-3-454
  • year: 1994
  • mesh: Animals, Base Composition, Cell Wall/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Deoxyribonuclease EcoRI, Food Microbiology, Humans, Mammals/microbiology, Nucleic Acid Hybridization, Operon, Peptidoglycan/analysis, RNA, Bacterial/*genetics, RNA, Ribosomal/*genetics, Species Specificity, Staphylococcus/*classification/*genetics/isolation & purification, Teichoic Acids/analysis
  • topic2: Biotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5933Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15615)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15615
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42178Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16804
51648Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33636)https://www.ccug.se/strain?id=33636
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
83786Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42827.1StrainInfo: A central database for resolving microbial strain identifiers
123881Curators of the CIPCollection of Institut Pasteur (CIP 104850)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104850