Strain identifier

BacDive ID: 14658

Type strain: No

Species: Mammaliicoccus vitulinus

Strain Designation: B92/78, NT215

Strain history: CIP <- 1995, PCM, Staphylococcus pulvereri <- Köln Univ., Poland

NCBI tax ID(s): 71237 (species)

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General

@ref: 3695

BacDive-ID: 14658

DSM-Number: 9930

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, Gram-positive, human pathogen

description: Mammaliicoccus vitulinus B92/78 is a facultative anaerobe, Gram-positive human pathogen that was isolated from human hip infection.

NCBI tax id

  • NCBI tax id: 71237
  • Matching level: species

strain history

@refhistory
3695<- PCM
123880CIP <- 1995, PCM, Staphylococcus pulvereri <- Köln Univ., Poland

doi: 10.13145/bacdive14658.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Mammaliicoccus
  • species: Mammaliicoccus vitulinus
  • full scientific name: Mammaliicoccus vitulinus (Webster et al. 1994) Madhaiyan et al. 2020

  • synonyms

    @refsynonym
    20215Staphylococcus vitulus
    20215Staphylococcus pulvereri
    20215Staphylococcus vitulinus

@ref: 3695

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Mammaliicoccus

species: Mammaliicoccus vitulinus

full scientific name: Mammaliicoccus vitulinus (Webster et al. 1994) Madhaiyan et al. 2020

strain designation: B92/78, NT215

type strain: no

Morphology

cell morphology

  • @ref: 123880
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis ability
3695alpha/beta1
1238801

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3695COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
3695TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34686MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123880CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
3695positivegrowth37
34686positivegrowth37
123880positivegrowth10-41
123880nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 123880
  • oxygen tolerance: facultative anaerobe

murein

  • @ref: 3695
  • murein short key: A11.08
  • type: A3alpha L-Lys-L-Ala-Gly4-5

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
123880esculin-hydrolysis4853
123880hippurate+hydrolysis606565
123880nitrate+reduction17632
123880nitrite-reduction16301
123880tributyrin-hydrolysis35020
123880nitrate-respiration17632
68375urea-hydrolysis16199
68375arginine-hydrolysis29016
68375ornithine-degradation18257
68375esculin-hydrolysis4853
68375D-glucose+fermentation17634
68375D-fructose+fermentation15824
68375D-mannose-fermentation16024
68375maltose-fermentation17306
68375lactose-fermentation17716
68375trehalose+fermentation27082
68375D-mannitol+fermentation16899
68375raffinose-fermentation16634
68375D-ribose-fermentation16988
68375cellobiose-fermentation17057
68375nitrate+reduction17632
68375sucrose+fermentation17992
68375N-acetylglucosamine-fermentation59640
68375turanose-fermentation32528
68375arabinose-fermentation22599

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is resistant: yes
  • resistance conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinyes
12388035581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
6837515688acetoin+
12388015688acetoin-
12388017234glucose-

enzymes

@refvalueactivityec
3695catalase+1.11.1.6
3695cytochrome-c oxidase+1.9.3.1
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase-3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5
123880oxidase+
123880beta-galactosidase-3.2.1.23
123880alcohol dehydrogenase-1.1.1.1
123880gelatinase+/-
123880amylase-
123880DNase-
123880caseinase-3.4.21.50
123880catalase+1.11.1.6
123880coagulase-
123880tween esterase-
123880gamma-glutamyltransferase-2.3.2.2
123880lecithinase-
123880lipase-
123880lysine decarboxylase-4.1.1.18
123880ornithine decarboxylase-4.1.1.17
123880phenylalanine ammonia-lyase-4.3.1.24
123880protease-
123880urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    51841C14:00.414
    51841C16:00.416
    51841C13:0 ANTEISO1.412.701
    51841C13:0 iso2.912.612
    51841C14:0 ISO213.618
    51841C15:0 ANTEISO4314.711
    51841C15:0 ISO35.914.621
    51841C15:1 ISO AT 50.414.387
    51841C16:1 ω11c115.757
    51841C16:1 ω7c alcohol0.915.386
    51841C17:0 anteiso216.722
    51841C17:0 iso116.629
    51841C17:1 ISO I/C16:0 DMA2.216.481
    51841C17:1 ω10c ISO3.416.387
    51841C18:2 ω6,9c/C18:0 ANTE0.917.724
    51841Unidentified0.516.3
    51841Unidentified0.418.217
    51841unknown 15.6651.215.665
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123880-+++-+----+----++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123880+---++/-----+++----+---------+/---++------++---------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
3695----++---++---++----++----

Isolation, sampling and environmental information

isolation

@refsample type
3695human hip infection
51841Hip infection
123880Hip infection

isolation source categories

Cat1Cat2
#Infection#Disease
#Host#Human

taxonmaps

  • @ref: 69479
  • File name: preview.99_4196.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_96;98_105;99_4196&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: AB009942
  • Sequence Identity:
  • Total samples: 998
  • soil counts: 25
  • aquatic counts: 30
  • animal counts: 892
  • plant counts: 51

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
3695yes, in single cases1Risk group (German classification)
1238801Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus vitulinus rrn gene for 16S ribosomal RNAAB0099421497nuccore71237
20218Staphylococcus pulvereri strain DSM 9930 16S ribosomal RNA gene, partial sequenceAY688086465nuccore71237
3695Staphylococcus pulvereri NT165 16S ribosomal RNA, partial sequenceU127641009nuccore33947

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mammaliicoccus vitulinus DSM 9930GCA_003970525scaffoldncbi71237
66792Mammaliicoccus vitulinus CCM 4481GCA_014635645scaffoldncbi71237
66792Mammaliicoccus vitulinus strain FDAARGOS_115371237.29completepatric71237
66792Staphylococcus vitulinus strain CCM 448171237.27wgspatric71237
66792Staphylococcus vitulinus strain DSM 993071237.20wgspatric71237

GC content

  • @ref: 3695
  • GC-content: 27
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes56no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes84.29no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no98.401yes
69480spore-formingspore-formingAbility to form endo- or exosporesno65.61no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes70.149yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.079yes
69480flagellatedmotile2+Ability to perform flagellated movementno79.882no

External links

@ref: 3695

culture collection no.: CCUG 33938, CIP 104364, CCM 4481, DSM 9930, ATCC 51698, PCM 2443

straininfo link

  • @ref: 83784
  • straininfo: 43129

literature

  • topic: Phylogeny
  • Pubmed-ID: 7857796
  • title: Staphylococcus pulvereri sp. nov., isolated from human and animal specimens.
  • authors: Zakrzewska-Czerwinska J, Gaszewska-Mastalarz A, Lis B, Gamian A, Mordarski M
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-45-1-169
  • year: 1995
  • mesh: Animals, Base Composition, Base Sequence, Humans, Molecular Sequence Data, RNA, Ribosomal, 16S/chemistry/genetics, Staphylococcus/chemistry/genetics/*isolation & purification
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3695Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9930)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9930
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34686Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16264
51841Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33938)https://www.ccug.se/strain?id=33938
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83784Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43129.1StrainInfo: A central database for resolving microbial strain identifiers
123880Curators of the CIPCollection of Institut Pasteur (CIP 104364)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104364