Strain identifier

BacDive ID: 14636

Type strain: Yes

Species: Staphylococcus hominis

Strain Designation: DM 122

Strain history: CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France, Staphylococcus hominis <- ATCC <- Kloos: strain DM 122

NCBI tax ID(s): 1290 (species)

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General

@ref: 8719

BacDive-ID: 14636

DSM-Number: 20328

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, coccus-shaped

description: Staphylococcus hominis DM 122 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from human skin.

NCBI tax id

  • NCBI tax id: 1290
  • Matching level: species

strain history

@refhistory
8719<- K.H. Schleifer <- W.E. Kloos, DM 122
67770DSM 20328 <-- K. H. Schleifer <-- W. E. Kloos; DM 122.
119956CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France, Staphylococcus hominis <- ATCC <- Kloos: strain DM 122

doi: 10.13145/bacdive14636.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus hominis
  • full scientific name: Staphylococcus hominis Kloos and Schleifer 1975 (Approved Lists 1980)

@ref: 8719

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus hominis subsp. hominis

full scientific name: Staphylococcus hominis subsp. hominis (Kloos and Schleifer 1975) Kloos et al. 1998

strain designation: DM 122

type strain: yes

Morphology

cell morphology

  • @ref: 119956
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: yes

colony morphology

@refincubation periodhemolysis ability
87191-2 days
1199561

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8719COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8719TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
32816MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119956CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
8719positivegrowth37mesophilic
32816positivegrowth37mesophilic
52786positivegrowth37mesophilic
67770positivegrowth37mesophilic
119956positivegrowth22-45
119956nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
52786aerobe
119956facultative anaerobe

murein

  • @ref: 8719
  • murein short key: A11.03
  • type: A3alpha L-Lys-Gly2-4-L-Ser1-2-Gly

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
119956606565hippurate-hydrolysis
11995617632nitrate+reduction
11995616301nitrite-reduction
6837516199urea+hydrolysis
6837529016arginine-hydrolysis
6837518257ornithine-degradation
683754853esculin-hydrolysis
6837517634D-glucose+fermentation
6837515824D-fructose+fermentation
6837516024D-mannose-fermentation
6837517306maltose+fermentation
6837517716lactose+fermentation
6837527082trehalose+fermentation
6837516634raffinose-fermentation
6837516988D-ribose-fermentation
6837517057cellobiose-fermentation
6837517632nitrate+reduction
6837517992sucrose+fermentation
6837532528turanose+fermentation

metabolite production

  • @ref: 119956
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 119956
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
8719catalase+1.11.1.6
8719cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase-3.2.1.31
68375L-arginine arylamidase-
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease+3.5.1.5
119956oxidase-
119956beta-galactosidase+3.2.1.23
119956alcohol dehydrogenase-1.1.1.1
119956gelatinase-
119956DNase-
119956catalase+1.11.1.6
119956coagulase-
119956tween esterase-
119956gamma-glutamyltransferase-2.3.2.2
119956lecithinase-
119956lysine decarboxylase-4.1.1.18
119956ornithine decarboxylase-4.1.1.17
119956urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119956-++++--+--++---+----

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8719+---++-+++----++---+/--++/-+--
8719+---++-+++----+-+/--+/-+/--+++--
8719+---++-+++----++-----+++--
8719+---++-+++----------+++--
8719+---++-+++----++-----+++--
8719+---++-+++----+------+++--
8719+---++-++++---+++---+/-++++-
8719+---++-+++----++-----+++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119956++++---+-++++------------------+----------------------------+--------------+-----------------------

Isolation, sampling and environmental information

isolation

@refsample type
8719human skin
52786Human skin
67770Human skin
119956Human, Skin

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Organ#Skin, Nail, Hair

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
87192Risk group (German classification)
1199562Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus hominis subsp. hominis strain DSM 20328 16S ribosomal RNA gene, partial sequenceAY688063464ena145391
20218Staphylococcus hominis 16S ribosomal RNA (16S rRNA) geneL376011468ena1290
20218S.hominis gene for 16S ribosomal RNAZ26905771ena1290
8719Staphylococcus hominis strain DM 122 16S ribosomal RNA, partial sequenceNR_0369561544nuccore1290
67770S.hominis gene for 16S ribosomal RNAX661011544ena1290

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus hominis strain NCTC113201290.133wgspatric1290
66792Staphylococcus hominis subsp. hominis strain NCTC 11320145391.8wgspatric145391
67770Staphylococcus hominis subsp. hominis NCTC 11320GCA_002901845scaffoldncbi145391
67770Staphylococcus hominis NCTC 11320GCA_900458635contigncbi1290

GC content

@refGC-contentmethod
871933.7
6777033.7thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno91.089no
gram-positiveyes91.396no
anaerobicno99.385no
aerobicyes89.821yes
halophileyes95.939no
spore-formingno91.542no
glucose-utilyes87.115no
glucose-fermentyes74.598no
flagellatedno93.212no
thermophileno99.515yes

External links

@ref: 8719

culture collection no.: DSM 20328, ATCC 27844, JCM 31912, CCUG 35516, BCRC 12156, CCM 3474, CECT 234, CIP 81.57, JCM 2419, KCTC 3343, LMG 13348, MTCC 6150, NBRC 110726, NCIMB 14488, NCTC 11320, NRRL B-14737, CCUG 6468, GIFU 9125

straininfo link

  • @ref: 83764
  • straininfo: 7700

literature

  • topic: Genetics
  • Pubmed-ID: 12700250
  • title: Identification in methicillin-susceptible Staphylococcus hominis of an active primordial mobile genetic element for the staphylococcal cassette chromosome mec of methicillin-resistant Staphylococcus aureus.
  • authors: Katayama Y, Takeuchi F, Ito T, Ma XX, Ui-Mizutani Y, Kobayashi I, Hiramatsu K
  • journal: J Bacteriol
  • DOI: 10.1128/JB.185.9.2711-2722.2003
  • year: 2003
  • mesh: Amino Acid Sequence, Chromosomes, Bacterial/*genetics, Gene Transfer, Horizontal, *Genes, Bacterial, Methicillin/pharmacology, Methicillin Resistance/*genetics, Molecular Sequence Data, Penicillins/pharmacology, Sequence Alignment, Species Specificity, Staphylococcus aureus/drug effects/*genetics, Staphylococcus hominis/drug effects/*genetics
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8719Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20328)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20328
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32816Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11220
52786Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 35516)https://www.ccug.se/strain?id=35516
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
83764Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7700.1StrainInfo: A central database for resolving microbial strain identifiers
119956Curators of the CIPCollection of Institut Pasteur (CIP 81.57)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.57