Strain identifier

BacDive ID: 14631

Type strain: Yes

Species: Mammaliicoccus sciuri

Strain history: CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France <- ATCC <- W.E. Kloos: strain SC116, Sciurus carolinensis

NCBI tax ID(s): 1296 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 8750

BacDive-ID: 14631

DSM-Number: 20345

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, human pathogen

description: Mammaliicoccus sciuri DSM 20345 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from skin of eastern gray squirrel .

NCBI tax id

  • NCBI tax id: 1296
  • Matching level: species

strain history

@refhistory
8750<- K.H. Schleifer <- W.E. Kloos, SC 116
67770ATCC 29062 <-- W. E. Kloos SC 116.
122519CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France <- ATCC <- W.E. Kloos: strain SC116, Sciurus carolinensis

doi: 10.13145/bacdive14631.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Mammaliicoccus
  • species: Mammaliicoccus sciuri
  • full scientific name: Mammaliicoccus sciuri (Kloos et al. 1976) Madhaiyan et al. 2020

  • synonyms

    @refsynonym
    20215Staphylococcus sciuri
    20215Staphylococcus sciuri subsp. rodentium
    20215Staphylococcus sciuri subsp. carnaticus

@ref: 8750

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Staphylococcaceae

genus: Mammaliicoccus

species: Mammaliicoccus sciuri

full scientific name: Mammaliicoccus sciuri (Kloos et al. 1976) Madhaiyan et al. 2020

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
122519positivecoccus-shapedno

colony morphology

@reftype of hemolysisincubation period
8750gamma1-2 days
122519

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8750COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8750TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
42159MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122519CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
8750positivegrowth37mesophilic
42159positivegrowth37mesophilic
46285positivegrowth37mesophilic
67770positivegrowth37mesophilic
122519positivegrowth10-45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
46285aerobe
122519facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.683

murein

  • @ref: 8750
  • murein short key: A11.08
  • type: A3alpha L-Lys-L-Ala-Gly4-5

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose+builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
122519hippurate+hydrolysis606565
122519nitrate+reduction17632
122519nitrite-reduction16301
68375urea-hydrolysis16199
68375arginine-hydrolysis29016
68375ornithine-degradation18257
68375esculin+hydrolysis4853
68375D-glucose+fermentation17634
68375D-fructose+fermentation15824
68375D-mannose+fermentation16024
68375maltose+fermentation17306
68375lactose+fermentation17716
68375trehalose+fermentation27082
68375D-mannitol+fermentation16899
68375raffinose-fermentation16634
68375D-ribose+fermentation16988
68375cellobiose+fermentation17057
68375nitrate+reduction17632
68375sucrose+fermentation17992
68375N-acetylglucosamine+fermentation59640
68375turanose+fermentation32528
68375arabinose+fermentation22599

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is resistant: yes
  • resistance conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinno
12251935581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6837515688acetoin-
12251915688acetoin-

enzymes

@refvalueactivityec
8750catalase+1.11.1.6
8750cytochrome-c oxidase+1.9.3.1
68375beta-glucuronidase+3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase+3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5
122519oxidase+
122519beta-galactosidase-3.2.1.23
122519alcohol dehydrogenase-1.1.1.1
122519gelatinase+
122519amylase-
122519DNase+/-
122519caseinase+3.4.21.50
122519catalase+1.11.1.6
122519coagulase-
122519tween esterase-
122519gamma-glutamyltransferase-2.3.2.2
122519lecithinase-
122519lipase-
122519lysine decarboxylase-4.1.1.18
122519ornithine decarboxylase-4.1.1.17
122519phenylalanine ammonia-lyase-4.3.1.24
122519protease+
122519urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122519-+++-+----++--++++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122519+--++----+/-+++----++/---+/-+++++/-+--++-+----++/---+/-------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8750---++++++++-+++---+-++++++
8750---++++++++-+++-----++++++
8750---++++++++-+++---+-++++++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122519+++++--+-+++---++++++-+--++-+--++++++----+---+---+---++----++------------+-+---++-----+---+++++---+

Isolation, sampling and environmental information

isolation

@refsample typehost speciessampling dategeographic locationcountryorigin.countrycontinentisolation date
8750skin of eastern gray squirrel (Sciurus carolinensis)Sciurus carolinensis
46285Eastern gray squirrel skin1988-06-15
67770Eastern grey squirrel skin
122519Eastern gray squirrel skinSunbury, PennsylvaniaUnited States of AmericaUSANorth America1972

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Rodentia (Other)
#Host Body-Site#Organ#Skin, Nail, Hair

taxonmaps

  • @ref: 69479
  • File name: preview.99_116.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_96;98_105;99_116&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: AJ421446
  • Sequence Identity:
  • Total samples: 7505
  • soil counts: 269
  • aquatic counts: 251
  • animal counts: 6869
  • plant counts: 116

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
8750yes, in single casesyes1Risk group (German classification)
1225191Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus sciuri 16S ribosomal RNA gene, partial sequenceAF041358906ena1296
20218Staphylococcus sciuri subsp. sciuri strain DSM 20345 16S ribosomal RNA gene, partial sequenceAY688097465ena147467
8750Staphylococcus sciuri subsp. sciuri 16S rRNA gene, strain DSM 20345TAJ4214461528ena147467

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mammaliicoccus sciuri NCTC12103GCA_900474615completencbi1296
66792Staphylococcus sciuri subsp. sciuri strain DSM 20345147467.8wgspatric1296
66792Staphylococcus sciuri subsp. sciuri strain NCTC 12103147467.21wgspatric1296
66792Staphylococcus sciuri subsp. sciuri strain NCTC12103147467.22completepatric1296
66792Staphylococcus sciuri sciuri DSM 203452627854141draftimg147467
67770Mammaliicoccus sciuri DSM 20345GCA_001046995contigncbi1296
67770Mammaliicoccus sciuri NCTC 12103GCA_002901825scaffoldncbi1296

GC content

@refGC-contentmethod
875036.5
6777032.7genome sequence analysis
6777036.5thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno85no
flagellatedno92.944no
gram-positiveyes92.402no
anaerobicno99.692yes
aerobicyes94.117yes
halophileyes94.899no
spore-formingno87.476no
thermophileno99.889yes
glucose-utilyes88.506no
motileno86.634no
glucose-fermentyes74.795yes

External links

@ref: 8750

culture collection no.: DSM 20345, ATCC 29062, BCRC 12927, CCM 3473, CCUG 15598, CNCTC 5683, JCM 2425, NCTC 12103, SC 116, CIP 81.62, MTCC 6154, NRRL B-14777

straininfo link

  • @ref: 83759
  • straininfo: 105433

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology9440511Genetic organization of the mecA region in methicillin-susceptible and methicillin-resistant strains of Staphylococcus sciuri.Wu S, de Lencastre H, Tomasz AJ Bacteriol10.1128/JB.180.2.236-242.19981998Amino Acid Sequence, *Bacterial Proteins, Base Sequence, Carrier Proteins/*genetics, Cloning, Molecular, DNA Fingerprinting, *Hexosyltransferases, Methicillin Resistance/*genetics, Microbial Sensitivity Tests, Molecular Sequence Data, Muramoylpentapeptide Carboxypeptidase/*genetics, Penicillin-Binding Proteins, *Peptidyl Transferases, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Staphylococcus/*geneticsGenetics
Phylogeny27654339High intraspecies heterogeneity within Staphylococcus sciuri and rejection of its classification into S. sciuri subsp. sciuri, S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium.Svec P, Petras P, Pantucek R, Doskar J, Sedlacek IInt J Syst Evol Microbiol10.1099/ijsem.0.0014932016Base Composition, DNA, Bacterial/genetics, *Phylogeny, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNA, Staphylococcus/*classification/geneticsTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8750Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20345)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20345
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42159Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11226
46285Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 15598)https://www.ccug.se/strain?id=15598
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
83759Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID105433.1StrainInfo: A central database for resolving microbial strain identifiers
122519Curators of the CIPCollection of Institut Pasteur (CIP 81.62)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.62