Strain identifier

BacDive ID: 14614

Type strain: Yes

Species: Staphylococcus lutrae

Strain Designation: M340/94

Strain history: CIP <- 1998, G. Foster, Vet. Serv., Inverness, UK: strain M340/94

NCBI tax ID(s): 155085 (species)

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General

@ref: 3864

BacDive-ID: 14614

DSM-Number: 10244

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped, animal pathogen

description: Staphylococcus lutrae M340/94 is an aerobe, mesophilic, Gram-positive animal pathogen that was isolated from mammary gland of otter .

NCBI tax id

  • NCBI tax id: 155085
  • Matching level: species

strain history

@refhistory
3864<- G. Foster, M340/94
326971998, G. Foster, Vet. Serv., Inverness, UK: strain M340/94
120713CIP <- 1998, G. Foster, Vet. Serv., Inverness, UK: strain M340/94

doi: 10.13145/bacdive14614.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus lutrae
  • full scientific name: Staphylococcus lutrae Foster et al. 1997

@ref: 3864

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus lutrae

full scientific name: Staphylococcus lutrae Foster et al. 1997

strain designation: M340/94

type strain: yes

Morphology

cell morphology

  • @ref: 120713
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis abilityincubation period
3864beta11-2 days
120713

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3864COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
3864TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
32697MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
120713CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
3864positivegrowth37mesophilic
32697positivegrowth37mesophilic
54290positivegrowth37mesophilic
120713positivegrowth25-41
120713nogrowth10psychrophilic
120713nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
54290aerobe
120713facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose+builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
120713hippurate-hydrolysis606565
120713nitrate+reduction17632
120713nitrite-reduction16301
68375urea+hydrolysis16199
68375arginine-hydrolysis29016
68375ornithine-degradation18257
68375esculin-hydrolysis4853
68375D-glucose+fermentation17634
68375D-fructose+fermentation15824
68375D-mannose+fermentation16024
68375maltose-fermentation17306
68375lactose+fermentation17716
68375trehalose+fermentation27082
68375D-mannitol+fermentation16899
68375raffinose-fermentation16634
68375D-ribose-fermentation16988
68375cellobiose-fermentation17057
68375nitrate+reduction17632
68375sucrose-fermentation17992
68375N-acetylglucosamine+fermentation59640
68375turanose-fermentation32528
68375arabinose-fermentation22599

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is sensitive: yes
  • sensitivity conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinno
12071335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6837515688acetoin-
12071315688acetoin-

enzymes

@refvalueactivityec
3864catalase+1.11.1.6
3864cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase-3.2.1.31
68375alkaline phosphatase+3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase+3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease+3.5.1.5
120713oxidase-
120713beta-galactosidase+3.2.1.23
120713alcohol dehydrogenase-1.1.1.1
120713gelatinase+/-
120713amylase-
120713DNase+
120713caseinase+3.4.21.50
120713catalase+1.11.1.6
120713coagulase+
120713tween esterase-
120713gamma-glutamyltransferase-2.3.2.2
120713lecithinase+
120713lipase+
120713lysine decarboxylase-4.1.1.18
120713ornithine decarboxylase-4.1.1.17
120713phenylalanine ammonia-lyase-4.3.1.24
120713urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase+3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120713-++++-----++-+-----+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120713+---+----++++----+---+------+--+-----------+-----

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
3864+---+++-+++---+-+-++/---+---
3864+---+++-+++---+-+-++--+---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120713+++++------++------+----+------+++---------------+---------+---------------+--+++-----+---+++++---+

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentsampling dateisolation date
3864mammary gland of otter (Lutra lutra)Lutra lutraScotland, Isle of SkyeUnited KingdomGBREurope
54290Otter,mammary gland and supramammary lymph nodeIsle of SkyeUnited KingdomGBREurope1994
120713Otter, Lutra lutra, mammary glandIsle of Skye, ScotlandUnited KingdomGBREurope1994

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Canidae (Dog)
#Host Body-Site#Organ#Mammary gland

taxonmaps

  • @ref: 69479
  • File name: preview.99_1134.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_3;98_888;99_1134&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: X84731
  • Sequence Identity:
  • Total samples: 413
  • soil counts: 3
  • aquatic counts: 5
  • animal counts: 399
  • plant counts: 6

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
3864yes2Risk group (German classification)
1207131Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus lutrae strain DSM 10244 16S ribosomal RNA gene, partial sequenceAY688077464ena155085
3864Staphylococcus sp. gene for 16S ribosomal RNAX847311511ena29387

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus lutrae DSM 10244GCA_002902165scaffoldncbi155085
66792Staphylococcus lutrae strain DSM 10244155085.7wgspatric155085
66792Staphylococcus lutrae DSM 102442917434650draftimg155085

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno93.223no
gram-positiveyes90.965no
anaerobicno99.212yes
halophileyes88.45no
spore-formingno87.486no
glucose-utilyes87.581no
aerobicyes89.631yes
thermophileno99.606yes
motileno90.548no
glucose-fermentyes81.42yes

External links

@ref: 3864

culture collection no.: DSM 10244, CCUG 38494, CIP 105399, CCM 4681, ATCC 700373

straininfo link

  • @ref: 83743
  • straininfo: 43900

literature

  • topic: Phylogeny
  • Pubmed-ID: 9226903
  • title: Staphylococcus lutrae sp. nov., a new coagulase-positive species isolated from otters.
  • authors: Foster G, Ross HM, Hutson RA, Collins MD
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-47-3-724
  • year: 1997
  • mesh: Animals, Coagulase/*metabolism, DNA, Bacterial/analysis, Molecular Sequence Data, Otters/*microbiology, Phylogeny, RNA, Ribosomal, 16S/analysis, Scotland, Staphylococcal Infections/microbiology/*veterinary, Staphylococcus/*classification/enzymology/genetics
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3864Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10244)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10244
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32697Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17414
54290Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 38494)https://www.ccug.se/strain?id=38494
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83743Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43900.1StrainInfo: A central database for resolving microbial strain identifiers
120713Curators of the CIPCollection of Institut Pasteur (CIP 105399)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105399