Strain identifier
BacDive ID: 14605
Type strain:
Species: Staphylococcus muscae
Strain Designation: MB4
Strain history: CIP <- 1993, V. Hajek, Palacky Univ., Olomouc, Czech Republic: strain MB4
NCBI tax ID(s): 1294 (species)
General
@ref: 2969
BacDive-ID: 14605
DSM-Number: 7068
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped
description: Staphylococcus muscae MB4 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from fly, body surface.
NCBI tax id
- NCBI tax id: 1294
- Matching level: species
strain history
@ref | history |
---|---|
2969 | <- CCM <- V. Hajek, MB4 |
40749 | 1993, V. Hajek, Inst. Med. Microbiol., Olomouc, Czechoslovakia: strain MB4 |
121204 | CIP <- 1993, V. Hajek, Palacky Univ., Olomouc, Czech Republic: strain MB4 |
doi: 10.13145/bacdive14605.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Staphylococcaceae
- genus: Staphylococcus
- species: Staphylococcus muscae
- full scientific name: Staphylococcus muscae Hájek et al. 1992
@ref: 2969
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Staphylococcaceae
genus: Staphylococcus
species: Staphylococcus muscae
full scientific name: Staphylococcus muscae Hájek et al. 1992
strain designation: MB4
type strain: yes
Morphology
cell morphology
- @ref: 121204
- gram stain: positive
- cell shape: coccus-shaped
- motility: no
colony morphology
@ref | type of hemolysis | hemolysis ability |
---|---|---|
2969 | beta | 1 |
121204 | 1 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
2969 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
2969 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
40749 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
121204 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2969 | positive | growth | 37 | mesophilic |
40749 | positive | growth | 37 | mesophilic |
53517 | positive | growth | 37 | mesophilic |
121204 | positive | growth | 37 | mesophilic |
121204 | no | growth | 15 | psychrophilic |
121204 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
53517 | aerobe |
121204 | facultative anaerobe |
murein
- @ref: 2969
- murein short key: A11.02
- type: A3alpha L-Lys-Gly5-6
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | + | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | maltose | - | builds acid from | 17306 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | esculin | - | builds acid from | 4853 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | - | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | - | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | - | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | + | builds acid from | 65327 |
68371 | D-ribose | - | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | + | builds acid from | 17754 |
121204 | nitrate | + | reduction | 17632 |
121204 | nitrite | - | reduction | 16301 |
121204 | tributyrin | - | hydrolysis | 35020 |
68375 | arginine | - | hydrolysis | 29016 |
68375 | ornithine | - | degradation | 18257 |
68375 | esculin | - | hydrolysis | 4853 |
68375 | D-mannose | - | fermentation | 16024 |
68375 | maltose | - | fermentation | 17306 |
68375 | raffinose | - | fermentation | 16634 |
68375 | D-ribose | - | fermentation | 16988 |
68375 | cellobiose | - | fermentation | 17057 |
68375 | nitrate | + | reduction | 17632 |
68375 | turanose | - | fermentation | 32528 |
68375 | arabinose | - | fermentation | 22599 |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. | is resistant |
---|---|---|---|---|---|---|
68375 | 28368 | novobiocin | yes | yes | 1.8 µg | |
121204 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) | yes | no | yes |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68375 | 15688 | acetoin | no |
121204 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test |
---|---|---|---|
68375 | 15688 | acetoin | - |
121204 | 15688 | acetoin | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68375 | beta-glucuronidase | + | 3.2.1.31 |
68375 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68375 | L-arginine arylamidase | - | |
68375 | beta-galactosidase | - | 3.2.1.23 |
68375 | beta-glucosidase | - | 3.2.1.21 |
68375 | ornithine decarboxylase | - | 4.1.1.17 |
68375 | arginine dihydrolase | - | 3.5.3.6 |
121204 | oxidase | - | |
121204 | beta-galactosidase | - | 3.2.1.23 |
121204 | alcohol dehydrogenase | - | 1.1.1.1 |
121204 | gelatinase | - | |
121204 | DNase | +/- | |
121204 | catalase | + | 1.11.1.6 |
121204 | coagulase | - | |
121204 | tween esterase | + | |
121204 | lecithinase | + | |
121204 | lysine decarboxylase | - | 4.1.1.18 |
121204 | ornithine decarboxylase | - | 4.1.1.17 |
121204 | urease | - | 3.5.1.5 |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121204 | + | - | - | - | - | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - |
API ID32STA
@ref | URE | ADH Arg | ODC | ESC | GLU | FRU | MNE | MAL | LAC | TRE | MAN | RAF | RIB | CEL | NIT | VP | beta GAL | ArgA | PAL | PyrA | NOVO | SAC | NAG | TUR | ARA | beta GUR |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2969 | - | - | - | - | +/- | +/- | - | - | - | +/- | - | - | - | - | + | - | - | - | + | - | - | +/- | +/- | - | - | + |
2969 | + | - | - | - | + | + | - | - | +/- | + | + | - | - | - | + | - | - | - | +/- | - | - | +/- | + | - | - | + |
2969 | - | - | - | - | + | + | - | - | - | +/- | - | - | - | - | + | - | - | - | - | - | - | +/- | +/- | - | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location |
---|---|---|---|---|---|
2969 | fly, body surface | Czech Republic | CZE | Europe | |
53517 | Fly body surface (Stomoxys calcitrans) | Czech Republic | CZE | Europe | Olomouc |
121204 | Other, Flies trapped in cow sheds | Czech Republic | CZE | Europe |
isolation source categories
- Cat1: #Host
- Cat2: #Arthropoda
- Cat3: #Insecta
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
2969 | 1 | Risk group (German classification) |
121204 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Staphylococcus muscae strain DSM 7068 16S ribosomal RNA gene, partial sequence | AY688079 | 464 | ena | 1294 |
2969 | Staphylococcus muscae partial 16S rRNA gene, type strain DSM7068T | FR733703 | 1537 | ena | 1294 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Staphylococcus muscae ATCC 49910 | GCA_003019275 | complete | ncbi | 1294 |
66792 | Staphylococcus muscae CCM 4175 | GCA_014634965 | scaffold | ncbi | 1294 |
66792 | Staphylococcus muscae DSM 7068 | GCA_002901905 | scaffold | ncbi | 1294 |
66792 | Staphylococcus muscae strain ATCC 49910 | 1294.8 | complete | patric | 1294 |
66792 | Staphylococcus muscae strain CCM 4175 | 1294.9 | wgs | patric | 1294 |
66792 | Staphylococcus muscae strain DSM 7068 | 1294.7 | wgs | patric | 1294 |
66792 | Staphylococcus muscae DSM 7068 | 2917496769 | draft | img | 1294 |
GC content
- @ref: 2969
- GC-content: 41.0
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 94.383 | no |
gram-positive | yes | 93.773 | no |
anaerobic | no | 98.489 | yes |
halophile | yes | 95.774 | no |
spore-forming | no | 91.192 | no |
glucose-util | yes | 86.657 | no |
aerobic | yes | 88.51 | yes |
thermophile | no | 99.664 | yes |
motile | no | 90.862 | no |
glucose-ferment | yes | 85.029 | no |
External links
@ref: 2969
culture collection no.: DSM 7068, ATCC 49910, CCM 4175, CCUG 36972, CIP 103641
straininfo link
- @ref: 83735
- straininfo: 42748
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 1371067 | Staphylococcus muscae, a new species isolated from flies. | Hajek V, Ludwig W, Schleifer KH, Springer N, Zitzelsberger W, Kroppenstedt RM, Kocur M | Int J Syst Bacteriol | 10.1099/00207713-42-1-97 | 1992 | Animals, Base Sequence, Diptera/*microbiology, Molecular Sequence Data, RNA, Bacterial/chemistry, RNA, Ribosomal, 16S/chemistry, Staphylococcus/classification/genetics/*isolation & purification | Genetics |
Phylogeny | 19819995 | Staphylococcus rostri sp. nov., a haemolytic bacterium isolated from the noses of healthy pigs. | Riesen A, Perreten V | Int J Syst Evol Microbiol | 10.1099/ijs.0.012443-0 | 2009 | Animals, Bacterial Proteins/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Hemolysin Proteins/metabolism, Hemolysis, Molecular Sequence Data, Nose/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Staphylococcus/*classification/genetics/*isolation & purification/metabolism, Sus scrofa/*microbiology, Switzerland | Metabolism |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
2969 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7068) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-7068 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
40749 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15461 | ||||
53517 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 36972) | https://www.ccug.se/strain?id=36972 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68371 | Automatically annotated from API 50CH acid | |||||
68375 | Automatically annotated from API ID32STA | |||||
83735 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42748.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121204 | Curators of the CIP | Collection of Institut Pasteur (CIP 103641) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103641 |