Strain identifier

BacDive ID: 14573

Type strain: No

Species: Staphylococcus simulans

Strain Designation: AW 215

Strain history: <- K.H. Schleifer <- W.E. Kloos, AW 215

NCBI tax ID(s): 1286 (species)

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General

@ref: 8714

BacDive-ID: 14573

DSM-Number: 20323

keywords: 16S sequence, Bacteria, mesophilic, human pathogen

description: Staphylococcus simulans AW 215 is a mesophilic human pathogen that was isolated from human skin.

NCBI tax id

  • NCBI tax id: 1286
  • Matching level: species

strain history

  • @ref: 8714
  • history: <- K.H. Schleifer <- W.E. Kloos, AW 215

doi: 10.13145/bacdive14573.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus simulans
  • full scientific name: Staphylococcus simulans Kloos and Schleifer 1975 (Approved Lists 1980)

@ref: 8714

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus simulans

full scientific name: Staphylococcus simulans Kloos and Schleifer 1975

strain designation: AW 215

type strain: no

Morphology

colony morphology

  • @ref: 8714
  • type of hemolysis: beta
  • hemolysis ability: 1
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8714COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8714TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 8714
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

murein

  • @ref: 8714
  • murein short key: A11.02
  • type: A3alpha L-Lys-Gly5-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837516199urea+hydrolysis
6837529016arginine+hydrolysis
6837518257ornithine-degradation
683754853esculin-hydrolysis
6837517634D-glucose+fermentation
6837515824D-fructose+fermentation
6837517306maltose-fermentation
6837517716lactose+fermentation
6837527082trehalose-fermentation
6837516899D-mannitol+fermentation
6837516634raffinose-fermentation
6837516988D-ribose-fermentation
6837517057cellobiose-fermentation
6837517632nitrate+reduction
6837517992sucrose+fermentation
6837559640N-acetylglucosamine+fermentation
6837532528turanose-fermentation
6837522599arabinose-fermentation

metabolite production

  • @ref: 68375
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

  • @ref: 68375
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
8714catalase+1.11.1.6
8714cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase+3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase-3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase+3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase+3.5.3.6
68375urease+3.5.1.5

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8714++--+++-+-+---+-+---+/-++--+
8714++--++--+-+---+-+----++--+
8714++--+++-+-+---+-+----++--+

Isolation, sampling and environmental information

isolation

  • @ref: 8714
  • sample type: human skin

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Organ#Skin, Nail, Hair

Safety information

risk assessment

  • @ref: 8714
  • pathogenicity human: yes, in single cases
  • pathogenicity animal: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Staphylococcus simulans strain DSM 20323 16S ribosomal RNA gene, partial sequence
  • accession: AY688100
  • length: 464
  • database: ena
  • NCBI tax ID: 1286

GC content

  • @ref: 8714
  • GC-content: 36.9

External links

@ref: 8714

culture collection no.: DSM 20323, ATCC 27849, CCM 2724

straininfo link

  • @ref: 83703
  • straininfo: 44790

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8714Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20323)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20323
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
68375Automatically annotated from API ID32STA
83703Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44790.1StrainInfo: A central database for resolving microbial strain identifiers