Strain identifier

BacDive ID: 14572

Type strain: Yes

Species: Staphylococcus simulans

Strain Designation: MK 148, MK148

Strain history: CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France <- ATCC <- W.E. Kloos: strain MK148

NCBI tax ID(s): 1286 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 8713

BacDive-ID: 14572

DSM-Number: 20322

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, human pathogen

description: Staphylococcus simulans MK 148 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from human skin.

NCBI tax id

  • NCBI tax id: 1286
  • Matching level: species

strain history

@refhistory
8713<- K.H. Schleifer <- W.E. Kloos, MK 148
67770Y. Kosako <-- R. Sakazaki <-- ATCC 27848 <-- W. E. Kloos MK 148.
122626CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France <- ATCC <- W.E. Kloos: strain MK148

doi: 10.13145/bacdive14572.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus simulans
  • full scientific name: Staphylococcus simulans Kloos and Schleifer 1975 (Approved Lists 1980)

@ref: 8713

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus simulans

full scientific name: Staphylococcus simulans Kloos and Schleifer 1975

strain designation: MK 148, MK148

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
122626negativecoccus-shapedno

colony morphology

@reftype of hemolysishemolysis ability
8713beta1
1226261

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8713COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8713TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34889MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
122626CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
8713positivegrowth37mesophilic
34889positivegrowth37mesophilic
44978positivegrowth37mesophilic
67770positivegrowth37mesophilic
122626positivegrowth10-45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44978aerobe
122626facultative anaerobe

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
8713Mueller-Hinton Agar371aerob34383838-4034444236-380>50103232-343422-2432-34383436122230-3220-22163628183442>50403803222-2434-36
8713Mueller-Hinton Agar26-28323032-3430404032-340>501030-3230-323220-2232343238-40142226181642-442818n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.
8713Mueller-Hinton Agar3726323030283836-383005010303232223236323612-14222616-1814302818n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.671

murein

  • @ref: 8713
  • murein short key: A11.02
  • type: A3alpha L-Lys-Gly5-6

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122626hippurate+hydrolysis606565
122626nitrate+reduction17632
122626nitrite-reduction16301
68375urea+hydrolysis16199
68375ornithine-degradation18257
68375esculin-hydrolysis4853
68375D-glucose+fermentation17634
68375D-fructose+fermentation15824
68375lactose+fermentation17716
68375trehalose+fermentation27082
68375D-mannitol+fermentation16899
68375raffinose-fermentation16634
68375nitrate+reduction17632
68375sucrose+fermentation17992
68375N-acetylglucosamine+fermentation59640
68375turanose-fermentation32528
68375arabinose-fermentation22599

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinno
12262635581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
6837515688acetoin-
12262615688acetoin-
12262617234glucose+

enzymes

@refvalueactivityec
8713catalase+1.11.1.6
8713cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase+3.2.1.31
68375L-arginine arylamidase-
68375beta-galactosidase+3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375urease+3.5.1.5
122626oxidase-
122626beta-galactosidase+3.2.1.23
122626alcohol dehydrogenase+1.1.1.1
122626gelatinase-
122626amylase-
122626DNase+/-
122626caseinase-3.4.21.50
122626catalase+1.11.1.6
122626coagulase-
122626tween esterase-
122626gamma-glutamyltransferase-2.3.2.2
122626lecithinase+
122626lipase-
122626lysine decarboxylase-4.1.1.18
122626ornithine decarboxylase-4.1.1.17
122626phenylalanine ammonia-lyase-4.3.1.24
122626protease+
122626urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122626--++------+--+++----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122626----+/------++---------+------+-++-----------------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8713++--++--+++---+-+--+-++--+
8713++--++--+++-+/--+-+-+/-++/-++--+
8713++--++--+++---+-+-+++++--+
8713++--+++++++-+++-+--++/-++--+
8713+---++--+++-+-+-+-+-+++--+
8713++--++--+++-+-+-+-+++++--+
8713++--++--+++-+-+-+-+++++--+
8713++--++--+++-+-+-+-+/-+-++--+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122626++-+---+---+-----------------------------------------------++--------------------------------------

Isolation, sampling and environmental information

isolation

@refsample type
8713human skin
44978Human skin
67770Human skin
122626Human, Skin

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Organ#Skin, Nail, Hair

taxonmaps

  • @ref: 69479
  • File name: preview.99_2513.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_1609;98_1955;99_2513&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: D83373
  • Sequence Identity:
  • Total samples: 296
  • soil counts: 17
  • aquatic counts: 18
  • animal counts: 249
  • plant counts: 12

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
8713yes, in single casesyes, in single cases1Risk group (German classification)
1226261Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus simulans strain DSM 20322 16S ribosomal RNA gene, partial sequenceAY688099464ena1286
8713Staphylococcus simulans gene for 16S rRNA, partial sequence, strain: ATCC 27848 (= MAFF 910161)D833731476ena1286

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus simulans NCTC11046GCA_900474685completencbi1286
66792Staphylococcus simulans Staphylococcus simulans ATCC 27848GCA_900240105chromosomencbi1286
66792Staphylococcus simulans strain NCTC 110461286.76wgspatric1286
66792Staphylococcus simulans strain NCTC110461286.118completepatric1286
66792Staphylococcus simulans strain Staphylococcus simulans ATCC 278481286.80completepatric1286
67770Staphylococcus simulans NCTC 11046GCA_002902285scaffoldncbi1286

GC content

@refGC-contentmethod
871334.0
6777034thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno83no
flagellatedno90.602no
gram-positiveyes91.11no
anaerobicno98.73yes
aerobicyes87.95yes
halophileyes93.73no
spore-formingno91.561no
thermophileno99.726yes
glucose-utilyes88.309no
motileno85.155no
glucose-fermentyes81.915yes

External links

@ref: 8713

culture collection no.: DSM 20322, ATCC 27848, CCM 2705, CCUG 7327 A, NCTC 11046, JCM 2424, BCRC 12928, CCUG 7327, CECT 4538, CIP 81.64, HAMBI 2058, NBRC 109714, NRRL B-14753, VTT E-97784, GIFU 9127

straininfo link

  • @ref: 83702
  • straininfo: 39214

literature

  • Pubmed-ID: 7315345
  • title: A polypeptide antigen from a strain of Staphylococcus simulans. 2. Antigenic and biological properties.
  • authors: Osland A, Sveen K
  • journal: Acta Pathol Microbiol Scand B
  • DOI: 10.1111/j.1699-0463.1981.tb00176_89b.x
  • year: 1981
  • mesh: Animals, Antibodies, Bacterial/biosynthesis, Antigens, Bacterial/*immunology, Bacterial Proteins/*immunology, Cell Migration Inhibition, Chemotaxis, Leukocyte, Complement C4/physiology, Mice, Peptides/immunology, Shwartzman Phenomenon, Staphylococcus/*immunology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8713Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20322)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20322
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34889Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11228
44978Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 7327 A)https://www.ccug.se/strain?id=7327
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
83702Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39214.1StrainInfo: A central database for resolving microbial strain identifiers
122626Curators of the CIPCollection of Institut Pasteur (CIP 81.64)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.64