Strain identifier

BacDive ID: 14572

Type strain: Yes

Species: Staphylococcus simulans

Strain Designation: MK 148

Strain history: Y. Kosako <-- R. Sakazaki <-- ATCC 27848 <-- W. E. Kloos MK 148.

NCBI tax ID(s): 1286 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8713

BacDive-ID: 14572

DSM-Number: 20322

keywords: human pathogen, mesophilic, aerobe, Bacteria, 16S sequence, genome sequence

description: Staphylococcus simulans MK 148 is an aerobe, mesophilic human pathogen that was isolated from human skin.

NCBI tax id

  • NCBI tax id: 1286
  • Matching level: species

strain history

doi: 10.13145/bacdive14572.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus simulans
  • full scientific name: Staphylococcus simulans Kloos and Schleifer 1975 (Approved Lists 1980)

@ref: 8713

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus simulans

full scientific name: Staphylococcus simulans Kloos and Schleifer 1975

strain designation: MK 148

type strain: yes

Morphology

colony morphology

  • @ref: 8713
  • type of hemolysis: beta

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8713COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://bacmedia.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8713TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://bacmedia.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34889MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)

culture temp

@refgrowthtypetemperaturerange
8713positivegrowth37mesophilic
34889positivegrowth37mesophilic
44978positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 44978
  • oxygen tolerance: aerobe

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
8713Mueller-Hinton Agar371aerob34383838-4034444236-380>50103232-343422-2432-34383436122230-3220-22163628183442>50403803222-2434-36
8713Mueller-Hinton Agar26-28323032-3430404032-340>501030-3230-323220-2232343238-40142226181642-442818n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.
8713Mueller-Hinton Agar3726323030283836-383005010303232223236323612-14222616-1814302818n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.

murein

  • @ref: 8713
  • murein short key: A11.02
  • type: A3alpha L-Lys-Gly5-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837522599arabinose-fermentation
6837532528turanose-fermentation
6837559640N-acetylglucosamine+fermentation
6837517992sucrose+fermentation
6837517632nitrate+reduction
6837516634raffinose-fermentation
6837516899D-mannitol+fermentation
6837527082trehalose+fermentation
6837517716lactose+fermentation
6837515824D-fructose+fermentation
6837517634D-glucose+fermentation
683754853esculin-hydrolysis
6837518257ornithine-degradation
6837516199urea+hydrolysis

metabolite production

  • @ref: 68375
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

  • @ref: 68375
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
8713catalase+1.11.1.6
8713cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase+3.2.1.31
68375L-arginine arylamidase-
68375beta-galactosidase+3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375urease+3.5.1.5

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8713++--++--+++---+-+--+-++--+
8713++--++--+++-+/--+-+-+/-++/-++--+
8713++--++--+++---+-+-+++++--+
8713++--+++++++-+++-+--++/-++--+
8713+---++--+++-+-+-+-+-+++--+
8713++--++--+++-+-+-+-+++++--+
8713++--++--+++-+-+-+-+++++--+
8713++--++--+++-+-+-+-+/-+-++--+

Isolation, sampling and environmental information

isolation

@refsample type
8713human skin
44978Human skin
67770Human skin

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Organ#Skin, Nail, Hair

Safety information

risk assessment

  • @ref: 8713
  • pathogenicity human: yes, in single cases
  • pathogenicity animal: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus simulans strain DSM 20322 16S ribosomal RNA gene, partial sequenceAY688099464ena1286
8713Staphylococcus simulans gene for 16S rRNA, partial sequence, strain: ATCC 27848 (= MAFF 910161)D833731476ena1286

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus simulans NCTC11046GCA_900474685completencbi1286
66792Staphylococcus simulans Staphylococcus simulans ATCC 27848GCA_900240105chromosomencbi1286
66792Staphylococcus simulans NCTC 11046GCA_002902285scaffoldncbi1286
66792Staphylococcus simulans strain NCTC 110461286.76wgspatric1286
66792Staphylococcus simulans strain NCTC110461286.118completepatric1286
66792Staphylococcus simulans strain Staphylococcus simulans ATCC 278481286.80completepatric1286
67770Staphylococcus simulans strain NCTC11046 genome assembly, chromosome: 1LS483313ena1286
67770Staphylococcus simulans isolate Staphylococcus simulans ATCC 27848 genome assembly, chromosome: ILT963435ena1286
67770Staphylococcus simulans strain NCTC 11046, whole genome shotgun sequencing projectPPRU00000000ncbi1286

GC content

@refGC-contentmethod
871334.0
6777034thermal denaturation, midpoint method (Tm)

External links

@ref: 8713

culture collection no.: DSM 20322, ATCC 27848, CCM 2705, CCUG 7327 A, NCTC 11046, JCM 2424, BCRC 12928, CCUG 7327, CECT 4538, CIP 81.64, HAMBI 2058, NBRC 109714, NRRL B-14753, VTT E-97784

straininfo link

@refpassport
20218http://www.straininfo.net/strains/144749
20218http://www.straininfo.net/strains/106052
20218http://www.straininfo.net/strains/144747

literature

  • Pubmed-ID: 7315345
  • title: A polypeptide antigen from a strain of Staphylococcus simulans. 2. Antigenic and biological properties.
  • authors: Osland A, Sveen K
  • journal: Acta Pathol Microbiol Scand B
  • DOI: 10.1111/j.1699-0463.1981.tb00176_89b.x
  • year: 1981
  • mesh: Animals, Antibodies, Bacterial/biosynthesis, Antigens, Bacterial/*immunology, Bacterial Proteins/*immunology, Cell Migration Inhibition, Chemotaxis, Leukocyte, Complement C4/physiology, Mice, Peptides/immunology, Shwartzman Phenomenon, Staphylococcus/*immunology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8713Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20322)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20322
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34889Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11228
44978Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 7327 A)https://www.ccug.se/strain?id=7327
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68375Automatically annotated from API ID32STA