Strain identifier

BacDive ID: 14572

Type strain: Yes

Species: Staphylococcus simulans

Strain Designation: MK 148, MK148

Strain history: CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France <- ATCC <- W.E. Kloos: strain MK148

NCBI tax ID(s): 1286 (species)

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General

@ref: 8713

BacDive-ID: 14572

DSM-Number: 20322

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, coccus-shaped

description: Staphylococcus simulans MK 148 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from human skin.

NCBI tax id

  • NCBI tax id: 1286
  • Matching level: species

strain history

@refhistory
8713<- K.H. Schleifer <- W.E. Kloos, MK 148
67770Y. Kosako <-- R. Sakazaki <-- ATCC 27848 <-- W. E. Kloos MK 148.
122626CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France <- ATCC <- W.E. Kloos: strain MK148

doi: 10.13145/bacdive14572.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus simulans
  • full scientific name: Staphylococcus simulans Kloos and Schleifer 1975 (Approved Lists 1980)

@ref: 8713

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus simulans

full scientific name: Staphylococcus simulans Kloos and Schleifer 1975

strain designation: MK 148, MK148

type strain: yes

Morphology

cell morphology

  • @ref: 122626
  • gram stain: negative
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis ability
8713beta1
1226261

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8713COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8713TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34889MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
122626CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
8713positivegrowth37
34889positivegrowth37
44978positivegrowth37
67770positivegrowth37
122626positivegrowth10-45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44978aerobe
122626facultative anaerobe

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
8713Mueller-Hinton Agar371aerob34383838-4034444236-380>50103232-343422-2432-34383436122230-3220-22163628183442>50403803222-2434-36
8713Mueller-Hinton Agar26-28323032-3430404032-340>501030-3230-323220-2232343238-40142226181642-442818n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.
8713Mueller-Hinton Agar3726323030283836-383005010303232223236323612-14222616-1814302818n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.

spore formation

  • @ref: 125439
  • spore formation: no
  • confidence: 97.5

murein

  • @ref: 8713
  • murein short key: A11.02
  • type: A3alpha L-Lys-Gly5-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837522599arabinose-fermentation
6837532528turanose-fermentation
6837559640N-acetylglucosamine+fermentation
6837517992sucrose+fermentation
6837517632nitrate+reduction
6837516899D-mannitol+fermentation
6837527082trehalose+fermentation
6837517716lactose+fermentation
6837515824D-fructose+fermentation
683754853esculin-hydrolysis
6837518257ornithine-degradation
6837516199urea+hydrolysis
122626606565hippurate+hydrolysis
12262617632nitrate+reduction
12262616301nitrite-reduction
68371Potassium 5-ketogluconate-builds acid from
6837127613amygdalin-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837516634raffinose-fermentation
6837517634D-glucose+fermentation
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose+builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinno
12262635581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
6837515688acetoin-
12262615688acetoin-
12262617234glucose+

enzymes

@refvalueactivityec
8713catalase+1.11.1.6
8713cytochrome-c oxidase-1.9.3.1
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382alkaline phosphatase-3.1.3.1
122626oxidase-
122626beta-galactosidase+3.2.1.23
122626alcohol dehydrogenase+1.1.1.1
122626gelatinase-
122626amylase-
122626DNase+/-
122626caseinase-3.4.21.50
122626catalase+1.11.1.6
122626coagulase-
122626tween esterase-
122626gamma-glutamyltransferase-2.3.2.2
122626lecithinase+
122626lipase-
122626lysine decarboxylase-4.1.1.18
122626ornithine decarboxylase-4.1.1.17
122626phenylalanine ammonia-lyase-4.3.1.24
122626protease+
122626urease+3.5.1.5
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375urease+3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382trypsin-3.4.21.4
68375beta-glucuronidase+3.2.1.31
68375L-arginine arylamidase-
68375beta-galactosidase+3.2.1.23

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122626--++------+--+++----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122626----+/------++---------+------+-++-----------------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8713++--++--+++---+-+--+-++--+
8713++--++--+++-+/--+-+-+/-++/-++--+
8713++--++--+++---+-+-+++++--+
8713++--+++++++-+++-+--++/-++--+
8713+---++--+++-+-+-+-+-+++--+
8713++--++--+++-+-+-+-+++++--+
8713++--++--+++-+-+-+-+++++--+
8713++--++--+++-+-+-+-+/-+-++--+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122626++-+---+---+-----------------------------------------------++--------------------------------------

Isolation, sampling and environmental information

isolation

@refsample type
8713human skin
44978Human skin
67770Human skin
122626Human, Skin

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Organ#Skin, Nail, Hair

taxonmaps

  • @ref: 69479
  • File name: preview.99_2513.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_1609;98_1955;99_2513&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: D83373
  • Sequence Identity:
  • Total samples: 296
  • soil counts: 17
  • aquatic counts: 18
  • animal counts: 249
  • plant counts: 12

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
8713yes, in single casesyes, in single cases1Risk group (German classification)
1226261Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus simulans strain DSM 20322 16S ribosomal RNA gene, partial sequenceAY688099464nuccore1286
8713Staphylococcus simulans gene for 16S rRNA, partial sequence, strain: ATCC 27848 (= MAFF 910161)D833731476nuccore1286

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus simulans NCTC11046GCA_900474685completencbi1286
66792Staphylococcus simulans Staphylococcus simulans ATCC 27848GCA_900240105chromosomencbi1286
66792Staphylococcus simulans strain NCTC 110461286.76wgspatric1286
66792Staphylococcus simulans strain NCTC110461286.118completepatric1286
66792Staphylococcus simulans strain Staphylococcus simulans ATCC 278481286.80completepatric1286
67770Staphylococcus simulans NCTC 11046GCA_002902285scaffoldncbi1286

GC content

@refGC-contentmethod
871334.0
6777034thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthaerobe87.1
125439BacteriaNetgram_stainReaction to gram-stainingpositive70.8
125439BacteriaNetmotilityAbility to perform movementno74
125439BacteriaNetspore_formationAbility to form endo- or exosporesno97.5
125438gram-positivegram-positivePositive reaction to Gram-stainingyes88.366no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no98.797yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes51.473no
125438spore-formingspore-formingAbility to form endo- or exosporesno70.056no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno94.5yes
125438motile2+flagellatedAbility to perform flagellated movementno80.911no

External links

@ref: 8713

culture collection no.: DSM 20322, ATCC 27848, CCM 2705, CCUG 7327 A, NCTC 11046, JCM 2424, BCRC 12928, CCUG 7327, CECT 4538, CIP 81.64, HAMBI 2058, NBRC 109714, NRRL B-14753, VTT E-97784, GIFU 9127

straininfo link

  • @ref: 83702
  • straininfo: 39214

literature

  • Pubmed-ID: 7315345
  • title: A polypeptide antigen from a strain of Staphylococcus simulans. 2. Antigenic and biological properties.
  • authors: Osland A, Sveen K
  • journal: Acta Pathol Microbiol Scand B
  • DOI: 10.1111/j.1699-0463.1981.tb00176_89b.x
  • year: 1981
  • mesh: Animals, Antibodies, Bacterial/biosynthesis, Antigens, Bacterial/*immunology, Bacterial Proteins/*immunology, Cell Migration Inhibition, Chemotaxis, Leukocyte, Complement C4/physiology, Mice, Peptides/immunology, Shwartzman Phenomenon, Staphylococcus/*immunology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8713Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20322)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20322
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34889Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11228
44978Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 7327 A)https://www.ccug.se/strain?id=7327
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83702Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39214.1StrainInfo: A central database for resolving microbial strain identifiers
122626Curators of the CIPCollection of Institut Pasteur (CIP 81.64)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.64
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1