Strain identifier

BacDive ID: 14568

Type strain: Yes

Species: Staphylococcus schleiferi

Strain Designation: N850274, N 850274

Strain history: CIP <- 1993, J. Freney, Lyon Med. Univ., Lyon, France: strain N 850274 <- J. Fleurette, E. Herriot Hosp., Lyon, France

NCBI tax ID(s): 1295 (species)

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General

@ref: 1830

BacDive-ID: 14568

DSM-Number: 4807

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, human pathogen

description: Staphylococcus schleiferi N850274 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from jugular catheter.

NCBI tax id

  • NCBI tax id: 1295
  • Matching level: species

strain history

@refhistory
1830<- J. Fleurette <- Centre National des Staphylocoques, Lyon N850274
122511CIP <- 1993, J. Freney, Lyon Med. Univ., Lyon, France: strain N 850274 <- J. Fleurette, E. Herriot Hosp., Lyon, France

doi: 10.13145/bacdive14568.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus schleiferi
  • full scientific name: Staphylococcus schleiferi Freney et al. 1988

@ref: 1830

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus schleiferi subsp. schleiferi

full scientific name: Staphylococcus schleiferi subsp. schleiferi (Freney et al. 1988) Igimi et al. 1990

strain designation: N850274, N 850274

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
122511positivecoccus-shapedno

colony morphology

  • @ref: 122511
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1830COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
1830TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34406MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
122511CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
122511CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
1830positivegrowth37mesophilic
34406positivegrowth37mesophilic
48178positivegrowth37mesophilic
122511positivegrowth30-45
122511nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
48178aerobe
122511facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.837

murein

  • @ref: 1830
  • murein short key: A11.02
  • type: A3alpha L-Lys-Gly5-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12251117632nitrate+reduction
12251116301nitrite-reduction
12251135020tributyrin-hydrolysis
6837516199urea-hydrolysis
6837529016arginine+hydrolysis
6837518257ornithine-degradation
683754853esculin-hydrolysis
6837517634D-glucose+fermentation
6837515824D-fructose+fermentation
6837516024D-mannose+fermentation
6837517306maltose-fermentation
6837517716lactose-fermentation
6837527082trehalose-fermentation
6837516899D-mannitol-fermentation
6837516634raffinose-fermentation
6837516988D-ribose-fermentation
6837517057cellobiose-fermentation
6837517632nitrate+reduction
6837517992sucrose-fermentation
6837532528turanose-fermentation
6837522599arabinose-fermentation

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is sensitive: yes
  • sensitivity conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinyes
12251135581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6837515688acetoin+
12251115688acetoin-

enzymes

@refvalueactivityec
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase+3.4.19.3
68375alkaline phosphatase+3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase+3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase+3.5.3.6
68375urease-3.5.1.5
122511oxidase-
122511beta-galactosidase+3.2.1.23
122511alcohol dehydrogenase-1.1.1.1
122511gelatinase+/-
122511DNase+
122511catalase+1.11.1.6
122511coagulase-
122511lecithinase+
122511lipase+
122511lysine decarboxylase-4.1.1.18
122511ornithine decarboxylase-4.1.1.17
122511urease-3.5.1.5

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
1830-+--+++-------+++-++--+/----
1830-+--+++-------+++-++--+---
1830-+--+++-------+++-++--+---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
1830jugular catheter
48178Jugular catheterLyon ?FranceFRAEurope
122511Other, Jugular catheterLyonFranceFRAEurope1985

isolation source categories

Cat1Cat2Cat3
#Infection#Medical device#Catheter
#Host Body-Site#Organ#Vascular system

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
1830yesyes2Risk group (German classification)
1225111Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus schleiferi subsp. schleiferi strain ATCC 43808 16S ribosomal RNA gene, partial sequenceEU157299366ena74707
20218S.schleiferi gene for 16S ribosomal RNAZ26904771ena1295
20218Staphylococcus schleiferi subsp. schleiferi strain DSM 4807 16S ribosomal RNA gene, partial sequenceAY688092464ena74707
183016S rRNA [Staphylococcus schleiferi, Genomic, 1554 nt]S835681554ena1295

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus schleiferi ATCC 43808GCA_011137195contigncbi1295
66792Staphylococcus schleiferi subsp. schleiferi NCTC 12218GCA_003968865scaffoldncbi74707
66792Staphylococcus schleiferi strain ATCC 438081295.80wgspatric1295
66792Staphylococcus schleiferi strain NCTC122181295.64wgspatric1295
66792Staphylococcus schleiferi subsp. schleiferi strain NCTC 1221874707.3wgspatric74707
66792Staphylococcus schleiferi NCTC12218GCA_900458895completencbi1295

GC content

  • @ref: 1830
  • GC-content: 37.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno62no
flagellatedno87.973no
flagellatedno87.973no
gram-positiveyes88.842no
gram-positiveyes88.842no
anaerobicno99.139yes
anaerobicno99.139yes
aerobicyes91.932yes
aerobicyes91.932yes
halophileyes91.94no
halophileyes91.94no
spore-formingno88.933no
spore-formingno88.933no
thermophileno99.843no
thermophileno99.843no
glucose-utilyes89.251no
glucose-utilyes89.251no
motileno88.231no
motileno88.231no
glucose-fermentyes85.591yes
glucose-fermentyes85.591yes

External links

@ref: 1830

culture collection no.: DSM 4807, ATCC 43808, CCUG 25351, NCTC 12218, CCM 4070, CIP 103643

straininfo link

  • @ref: 83698
  • straininfo: 7699

literature

  • topic: Phylogeny
  • Pubmed-ID: 2275856
  • title: Staphylococcus schleiferi subsp. coagulans subsp. nov., isolated from the external auditory meatus of dogs with external ear otitis.
  • authors: Igimi S, Takahashi E, Mitsuoka T
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-40-4-409
  • year: 1990
  • mesh: Animals, DNA, Bacterial/chemistry, Dog Diseases/*microbiology, Dogs, Ear, External/microbiology, Nucleic Acid Hybridization, Otitis Media/*microbiology/veterinary, Sequence Homology, Nucleic Acid, Staphylococcus/genetics/*isolation & purification
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1830Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4807)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4807
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34406Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15463
48178Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 25351)https://www.ccug.se/strain?id=25351
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68375Automatically annotated from API ID32STA
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
83698Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7699.1StrainInfo: A central database for resolving microbial strain identifiers
122511Curators of the CIPCollection of Institut Pasteur (CIP 103643)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103643