Strain identifier

BacDive ID: 14567

Type strain: Yes

Species: Staphylococcus saccharolyticus

Strain Designation: S1

Strain history: CIP <- 1989, DSM <- ATCC <- W.E.C. Moore, Blacksburg, USA: strain VPI 5661 <- Foubert: strain S1, Peptococcus saccharolyticus

NCBI tax ID(s): 33028 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 8621

BacDive-ID: 14567

DSM-Number: 20359

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, coccus-shaped

description: Staphylococcus saccharolyticus S1 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from plasma.

NCBI tax id

  • NCBI tax id: 33028
  • Matching level: species

strain history

@refhistory
8621<- ATCC <- E.L. Foubert, S1 <- S.A.E. Gunter
67770DSM 20359 <-- ATCC 14953 <-- W. E. Moore VPI 5661 <-- E. L. Foubert S1 <-- S. A. E. Gunter.
122337CIP <- 1989, DSM <- ATCC <- W.E.C. Moore, Blacksburg, USA: strain VPI 5661 <- Foubert: strain S1, Peptococcus saccharolyticus

doi: 10.13145/bacdive14567.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus saccharolyticus
  • full scientific name: Staphylococcus saccharolyticus (Foubert and Douglas 1948) Kilpper-Bälz and Schleifer 1984

  • synonyms

    @refsynonym
    20215Peptococcus saccharolyticus
    20215Micrococcus saccharolyticus

@ref: 8621

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus saccharolyticus

full scientific name: Staphylococcus saccharolyticus (Foubert and Douglas 1948) Kilpper-Bälz and Schleifer 1984

strain designation: S1

type strain: yes

Morphology

cell morphology

  • @ref: 122337
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 122337

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8621COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8621PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
34237MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
122337CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
8621positivegrowth37mesophilic
34237positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8621anaerobe
122337facultative anaerobe

murein

  • @ref: 8621
  • murein short key: A11.03
  • type: A3alpha L-Lys-Gly2-4-L-Ser1-2-Gly

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
122337nitrate+reduction17632
122337nitrite-reduction16301
122337tributyrin-hydrolysis35020
122337sodium thiosulfate-builds gas from132112
68375urea+hydrolysis16199
68375ornithine-degradation18257
68375esculin-hydrolysis4853
68375trehalose-fermentation27082
68375D-mannitol-fermentation16899
68375raffinose-fermentation16634
68375D-ribose-fermentation16988
68375cellobiose-fermentation17057
68375arabinose-fermentation22599

antibiotic resistance

  • @ref: 122337
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinyes
12233735581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6837515688acetoin+
12233715688acetoin+

enzymes

@refvalueactivityec
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375urease+3.5.1.5
122337oxidase-
122337beta-galactosidase+3.2.1.23
122337alcohol dehydrogenase+1.1.1.1
122337gelatinase-
122337DNase-
122337catalase+1.11.1.6
122337lecithinase-
122337lysine decarboxylase-4.1.1.18
122337ornithine decarboxylase-4.1.1.17
122337urease+3.5.1.5

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122337+---------+++----+/-------------+/-------------------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8621++--+++-------++----+-+---
8621+---++--------++----------
8621+---+++++-----++--+--+++--
8621+--------------+----------
8621+--------------+----------

Isolation, sampling and environmental information

isolation

@refsample type
8621plasma
67770Plasma
122337Human, Plasma

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Fluids

taxonmaps

  • @ref: 69479
  • File name: preview.99_86.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_3;98_3;99_86&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: NR_113405
  • Sequence Identity:
  • Total samples: 8633
  • soil counts: 44
  • aquatic counts: 284
  • animal counts: 8287
  • plant counts: 18

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
86212Risk group (German classification)
1223371Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus saccharolyticus 16S ribosomal RNA (16S rRNA) geneL376021527ena33028
20218Staphylococcus saccharolyticus ATCC 14953 16S rRNA gene, partial sequenceU02922398ena33028
20218Staphylococcus saccharolyticus strain DSM 20359 16S ribosomal RNA gene, partial sequenceAY688087464ena33028
20218Staphylococcus saccharolyticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 1768AB6466161512ena33028
8621Staphylococcus saccharolyticus strain JCM 1768 16S ribosomal RNA, partial sequenceNR_1134051512nuccore33028

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus saccharolyticus NCTC 11807GCA_003968885scaffoldncbi33028
66792Staphylococcus saccharolyticus strain FDAARGOS_107433028.25completepatric33028
66792Staphylococcus saccharolyticus strain NCTC 1180733028.10wgspatric33028
66792Staphylococcus saccharolyticus strain NCTC1180733028.9wgspatric33028
67770Staphylococcus saccharolyticus NCTC11807GCA_900458815contigncbi33028

GC content

  • @ref: 8621
  • GC-content: 34.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes90.516no
anaerobicno98.829yes
halophileyes93.395no
spore-formingno92.72no
glucose-utilyes89.049no
motileno92.214no
flagellatedno92.527no
aerobicyes84.124yes
thermophileno99.857yes
glucose-fermentyes82.723no

External links

@ref: 8621

culture collection no.: DSM 20359, ATCC 14953, JCM 1768, CCUG 24040, CCUG 9989, CIP 103275, NCTC 11807, NRRL B-14778, VPI 5661

straininfo link

  • @ref: 83697
  • straininfo: 36214

literature

  • topic: Cultivation
  • Pubmed-ID: 7077295
  • title: Stimulation of the growth of cutaneous strains of Peptococcus saccharolyticus by iron, haematin and blood.
  • authors: Strom MS, Douglas HC, Evans CA
  • journal: J Gen Microbiol
  • DOI: 10.1099/00221287-128-2-387
  • year: 1982
  • mesh: Aerobiosis, Anaerobiosis, *Blood, Culture Media, Ferric Compounds/*pharmacology, Heme/*analogs & derivatives, Hemin/*pharmacology, Humans, Iron/*pharmacology, Peptococcus/*growth & development, Skin/microbiology
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8621Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20359)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20359
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34237Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15054
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83697Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36214.1StrainInfo: A central database for resolving microbial strain identifiers
122337Curators of the CIPCollection of Institut Pasteur (CIP 103275)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103275