Strain identifier

BacDive ID: 14518

Type strain: Yes

Species: Staphylococcus cohnii

Strain Designation: GH 137, GH137

Strain history: CIP <- 1981, J. Pillet, Inst. Pasteur, Garches, France <- DSM <- K.H. Schleifer <- W.E. Kloos: strain GH137

NCBI tax ID(s): 29382 (species)

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General

@ref: 8669

BacDive-ID: 14518

DSM-Number: 20260

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, human pathogen

description: Staphylococcus cohnii GH 137 is a facultative anaerobe, mesophilic, Gram-positive human pathogen that was isolated from human skin.

NCBI tax id

  • NCBI tax id: 29382
  • Matching level: species

strain history

@refhistory
8669<- K.H. Schleifer <- W.E. Kloos, GH 137
67770Y. Kosako <-- R. Sakazaki <-- ATCC 29974 <-- W. E. Kloos GH 137.
118331CIP <- 1981, J. Pillet, Inst. Pasteur, Garches, France <- DSM <- K.H. Schleifer <- W.E. Kloos: strain GH137

doi: 10.13145/bacdive14518.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus cohnii
  • full scientific name: Staphylococcus cohnii Schleifer and Kloos 1975 (Approved Lists 1980)

@ref: 8669

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus cohnii

full scientific name: Staphylococcus cohnii Schleifer and Kloos 1975

strain designation: GH 137, GH137

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
118331positivecoccus-shapedno

colony morphology

@reftype of hemolysishemolysis ability
8669beta1
1183311

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8669COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8669TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
33536MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
118331CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
8669positivegrowth37mesophilic
33536positivegrowth37mesophilic
67770positivegrowth37mesophilic
118331positivegrowth22-41
118331nogrowth10psychrophilic
118331nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 118331
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69481no94
69480no98.948

murein

  • @ref: 8669
  • murein short key: A11.02
  • type: A3alpha L-Lys-Gly5-6

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose-builds acid from28066
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
118331hippurate-hydrolysis606565
118331nitrate-reduction17632
118331nitrite-reduction16301
68375urea-hydrolysis16199
68375arginine-hydrolysis29016
68375ornithine-degradation18257
68375esculin-hydrolysis4853
68375D-glucose+fermentation17634
68375D-fructose+fermentation15824
68375D-mannose-fermentation16024
68375maltose+fermentation17306
68375lactose-fermentation17716
68375trehalose+fermentation27082
68375D-mannitol+fermentation16899
68375raffinose-fermentation16634
68375D-ribose-fermentation16988
68375cellobiose-fermentation17057
68375nitrate-reduction17632
68375sucrose-fermentation17992
68375N-acetylglucosamine-fermentation59640
68375turanose-fermentation32528
68375arabinose-fermentation22599

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinno
11833135581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
6837515688acetoin-
11833115688acetoin-
11833117234glucose+

enzymes

@refvalueactivityec
8669catalase+1.11.1.6
8669cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase+3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5
118331oxidase-
118331beta-galactosidase-3.2.1.23
118331alcohol dehydrogenase-1.1.1.1
118331gelatinase-
118331amylase-
118331DNase-
118331caseinase-3.4.21.50
118331catalase+1.11.1.6
118331coagulase-
118331tween esterase+
118331lecithinase-
118331lipase-
118331lysine decarboxylase-4.1.1.18
118331ornithine decarboxylase+4.1.1.17
118331phenylalanine ammonia-lyase-4.3.1.24
118331protease-
118331urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118331-+++-+----++--++----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118331+---+-----++-----+---------+---+-----+/--+/-------+--

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8669----++-+-++-------+-+-----
8669----++-+-++-------+-+-----
8669----++-+-++-------+-------
8669----++-+-++-------+-+/------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118331++++-----++--------+--------+--+-+-------------------+------+------------+-+--+-------+----+---+---

Isolation, sampling and environmental information

isolation

@refsample type
8669human skin
67770Human skin
118331Human, Skin

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Organ#Skin, Nail, Hair

taxonmaps

  • @ref: 69479
  • File name: preview.99_1035.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_3;98_3;99_1035&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: D83361
  • Sequence Identity:
  • Total samples: 759
  • soil counts: 26
  • aquatic counts: 20
  • animal counts: 679
  • plant counts: 34

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
8669yes, in single cases1Risk group (German classification)
1183311Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus cohnii subsp. cohnii strain DSM 20260 16S ribosomal RNA gene, partial sequenceAY688045464ena74704
8669Staphylococcus cohnii subsp. cohnii gene for 16S rRNA, partial sequence, strain: ATCC 29974 (=MAFF 911487)D833611477ena74704

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus cohnii Staphylococcus cohnii ATCC 29974GCA_900240165chromosomencbi29382
66792Staphylococcus cohnii subsp. cohnii NBRC 109713GCA_007992675contigncbi74704
66792Staphylococcus cohnii strain NCTC1104129382.51wgspatric29382
66792Staphylococcus cohnii strain Staphylococcus cohnii ATCC 2997429382.25completepatric29382
66792Staphylococcus cohnii subsp. cohnii strain NBRC 10971374704.14wgspatric74704
66792Staphylococcus cohnii subsp. cohnii strain NCTC 1104174704.9wgspatric74704
67770Staphylococcus cohnii subsp. cohnii NCTC 11041GCA_002902365scaffoldncbi74704
67770Staphylococcus cohnii NCTC11041GCA_900458255contigncbi29382

GC content

@refGC-contentmethod
866937.1
6777036.7-37.7thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno94no
gram-positiveyes90.161no
anaerobicno99.327no
halophileyes95.809no
spore-formingno89.923no
glucose-utilyes87.394no
motileno86.749no
aerobicyes91.174no
thermophileno99.763yes
flagellatedno90.054no
glucose-fermentyes85.997no

External links

@ref: 8669

culture collection no.: DSM 20260, ATCC 29974, CCM 2736, JCM 2417, BCRC 12155, CCUG 35144, CCUG 6463, CCUG 7322, CIP 81.54, NBRC 109713, NCIMB 13637, NCTC 11041, NRRL B-14756

straininfo link

  • @ref: 83649
  • straininfo: 105431

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1854641Staphylococcus cohnii subspecies: Staphylococcus cohnii subsp. cohnii subsp. nov. and Staphylococcus cohnii subsp. urealyticum subsp. nov.Kloos WE, Wolfshohl JFInt J Syst Bacteriol10.1099/00207713-41-2-2841991Animals, Cell Wall/chemistry, DNA, Bacterial, Fatty Acids/metabolism, Humans, Lactose/metabolism, Microbial Sensitivity Tests, Nucleic Acid Hybridization, Peptidoglycan/analysis, Pigmentation, Primates/microbiology, Staphylococcus/*classification/enzymology/genetics/metabolism, Teichoic Acids/analysisMetabolism
Phylogeny14657137Staphylococcus nepalensis sp. nov., isolated from goats of the Himalayan region.Spergser J, Wieser M, Taubel M, Rossello-Mora RA, Rosengarten R, Busse HJInt J Syst Evol Microbiol10.1099/ijs.0.02646-02003Altitude, Animals, DNA Fingerprinting, Geography, Goats/*microbiology, India, Microbial Sensitivity Tests, Molecular Sequence Data, *Phylogeny, Polymerase Chain Reaction, Staphylococcus/*classification/genetics/isolation & purification/physiologyGenetics
Genetics33576800Comparative Genomics Suggests a Taxonomic Revision of the Staphylococcus cohnii Species Complex.Lavecchia A, Chiara M, De Virgilio C, Manzari C, Pazzani C, Horner D, Pesole G, Placido AGenome Biol Evol10.1093/gbe/evab0202021Genes, Bacterial, Genome, Bacterial, Genomics, Nucleic Acid Hybridization, Phylogeny, Staphylococcus/*classification/genetics/isolation & purification, Whole Genome SequencingPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8669Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20260)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20260
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33536Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11217
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83649Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID105431.1StrainInfo: A central database for resolving microbial strain identifiers
118331Curators of the CIPCollection of Institut Pasteur (CIP 81.54)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.54