Strain identifier

BacDive ID: 14450

Type strain: No

Species: Staphylococcus aureus

Strain history: CIP <- 1990, ATCC <- FDA, Washington, USA

NCBI tax ID(s): 1280 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1393

BacDive-ID: 14450

DSM-Number: 3463

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-positive, coccus-shaped, human pathogen

description: Staphylococcus aureus DSM 3463 is a facultative anaerobe, mesophilic, Gram-positive human pathogen of the family Staphylococcaceae.

NCBI tax id

  • NCBI tax id: 1280
  • Matching level: species

strain history

@refhistory
1393<- ATCC; ATCC 29737 <- U.S. Food and Drug Administration (FDA), Washington, D.C., USA;
116574CIP <- 1990, ATCC <- FDA, Washington, USA

doi: 10.13145/bacdive14450.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus aureus
  • full scientific name: Staphylococcus aureus Rosenbach 1884 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Staphylococcus aureus subsp. anaerobius

@ref: 1393

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus aureus

full scientific name: Staphylococcus aureus Rosenbach 1884

type strain: no

Morphology

cell morphology

  • @ref: 116574
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis abilityincubation period
1393beta11-2 days
1165741

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1393COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
1393TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
41344MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116574CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
1393positivegrowth37mesophilic
41344positivegrowth37mesophilic
116574positivegrowth15-37
116574nogrowth10psychrophilic
116574nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116574
  • oxygen tolerance: facultative anaerobe

antibiogram

@refmediumPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactamincubation temperature
1393Mueller-Hinton Agar44-46384246364442300503432242422-2430-3230282601222188262434-36303840-4234320282034
1393Mueller-Hinton Agar322630322826282003620201812102420202001012168141820n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.37

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
116574nitrate+reduction17632
116574nitrite-reduction16301
116574tributyrin-hydrolysis35020
68375arginine+hydrolysis29016
68375ornithine-degradation18257
68375esculin-hydrolysis4853
68375D-glucose+fermentation17634
68375D-fructose+fermentation15824
68375D-mannose+fermentation16024
68375maltose+fermentation17306
68375lactose+fermentation17716
68375trehalose+fermentation27082
68375D-mannitol+fermentation16899
68375raffinose-fermentation16634
68375cellobiose-fermentation17057
68375nitrate+reduction17632
68375sucrose+fermentation17992
68375N-acetylglucosamine+fermentation59640
68375arabinose-fermentation22599

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinyes
11657435581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6837515688acetoin+
11657415688acetoin+

enzymes

@refvalueactivityec
1393catalase+1.11.1.6
1393cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase-3.2.1.31
68375alkaline phosphatase+3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase+3.5.3.6
116574oxidase-
116574beta-galactosidase+3.2.1.23
116574alcohol dehydrogenase+1.1.1.1
116574gelatinase+/-
116574DNase+
116574catalase+1.11.1.6
116574coagulase+
116574lecithinase+
116574lysine decarboxylase-4.1.1.18
116574ornithine decarboxylase-4.1.1.17
116574urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116574-+++------+----+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116574+---+----++++----+---+-----++-++-------+---------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
1393++--+++++++---++--+--+++/---
1393-+--+++++++-+-++--++++++--

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
1393yesyes2Risk group (German classification)
1165742Risk group (French classification)

External links

@ref: 1393

culture collection no.: DSM 3463, ATCC 29737, CCUG 19207, CIP 103428, NCIMB 12703

straininfo link

  • @ref: 83593
  • straininfo: 4997

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
4042664Efficacy of topical chloramphenicol and tobramycin ophthalmic solutions in preventing severe Staphylococcus aureus keratitis in rabbits.Edwards JG, Schlech BACurr Eye Res10.3109/027136885090952481985Animals, Chloramphenicol/*administration & dosage/therapeutic use, Keratitis/drug therapy/*etiology, Ophthalmic Solutions, Rabbits, *Staphylococcal Infections, Tobramycin/*administration & dosage/therapeutic use
10861973Studies on antibacterial activity of Ficus racemosa Linn. leaf extract.Mandal SC, Saha BP, Pal MPhytother Res10.1002/1099-1573(200006)14:4<278::aid-ptr592>3.0.co;2-72000Anti-Bacterial Agents/*pharmacology, Bacillus/drug effects, Escherichia coli/drug effects, Microbial Sensitivity Tests, Plant Extracts/*pharmacology, Plant Leaves/chemistry, Plants, Medicinal/*chemistry, Pseudomonas aeruginosa/drug effects, Staphylococcus aureus/drug effects, Trees/*chemistryPathogenicity
11346296Antimicrobial activity in urine: effect on leukocyte count and bacterial culture results.Abu Shaqra QMNew Microbiol2001Anti-Bacterial Agents/administration & dosage/pharmacology/*urine, Colony Count, Microbial/*methods, Escherichia coli/drug effects/growth & development, Humans, Leukocyte Count/*methods, Microbial Sensitivity Tests, Reproducibility of Results, Staphylococcus aureus/drug effects/growth & development, Urinary Tract Infections/diagnosis/immunology/microbiology/urine, Urine/chemistry/*cytology/*microbiologyPathogenicity
17021070Genetic background and antibiotic resistance of Staphylococcus aureus strains isolated in the Republic of Georgia.Revazishvili T, Bakanidze L, Gomelauri T, Zhgenti E, Chanturia G, Kekelidze M, Rajanna C, Kreger A, Sulakvelidze AJ Clin Microbiol10.1128/JCM.01030-062006Anti-Bacterial Agents/*pharmacology, *Drug Resistance, Multiple, Bacterial, Georgia (Republic)/epidemiology, Humans, Phylogeny, Staphylococcal Infections/epidemiology/microbiology, Staphylococcus aureus/*drug effects/*geneticsPathogenicityPhylogeny
20235748Synthesis of 1-(4-methoxybenzyl)-3-cyclopropyl-1H-pyrazol-5-amine derivatives as antimicrobial agents.Raju H, Nagamani TS, Chandrappa S, Ananda H, Vinaya K, Thimmegowda NR, Byregowda SM, Rangappa KSJ Enzyme Inhib Med Chem10.3109/147563609033576012010Anti-Bacterial Agents, Anti-Infective Agents/*chemical synthesis/chemistry/*pharmacology, Antifungal Agents, Benzamides/*chemical synthesis/pharmacology, Fungi/drug effects, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects, Microbial Sensitivity Tests, Pyrazoles, Structure-Activity RelationshipPathogenicity
23407707An Enhancing Effect of Gold Nanoparticles on the Lethal Action of 2450 MHz Electromagnetic Radiation in Microwave Oven.Mollazadeh-Moghaddam K, Moradi BV, Dolatabadi-Bazaz R, Shakibae M, Shahverdi ARAvicenna J Med Biotechnol2011
30719037Total Phenolic Content and Antioxidant and Antibacterial Activities of Pereskia bleo.Johari MA, Khong HYAdv Pharmacol Sci10.1155/2019/74285932019
32202302Molecular detection of methicillin heat-resistant Staphylococcus aureus strains in pasteurized camel milk in Saudi Arabia.Aljahani AH, Alarjani KM, Hassan ZK, Elkhadragy MF, Ismail EA, Al-Masoud AH, Yehia HMBiosci Rep10.1042/BSR201934702020Animals, Bacterial Proteins/genetics/isolation & purification, Camelus, Cefoxitin/pharmacology/therapeutic use, DNA, Bacterial/isolation & purification, *Hot Temperature, Humans, Methicillin-Resistant Staphylococcus aureus/drug effects/genetics/*isolation & purification, Microbial Sensitivity Tests/methods, Milk/*microbiology, Pasteurization/*methods, Penicillin-Binding Proteins/genetics/isolation & purification, Polymerase Chain Reaction, Saudi Arabia, Staphylococcal Food Poisoning/drug therapy/microbiology/*prevention & controlEnzymologyPhylogeny
32359985Prevalence of methicillin-resistant (mecA gene) and heat-resistant Staphylococcus aureus strains in pasteurized camel milk.Yehia HM, Al-Masoud AH, Alarjani KM, Alamri MSJ Dairy Sci10.3168/jds.2019-176312020Animals, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/*genetics, Camelus/*microbiology, Cefoxitin/pharmacology, Female, Hot Temperature, Methicillin/pharmacology, *Methicillin Resistance, Methicillin-Resistant Staphylococcus aureus/drug effects/genetics/*isolation & purification/physiology, Milk/*microbiology, Penicillin-Binding Proteins/*genetics, Saudi Arabia, ThermotoleranceStressEnzymology
35421124Inhibitory potentials of Streptomyces exfoliatus strain 'MUJA10' against bacterial pathogens isolated from rural areas in Riyadh, Saudi Arabia.Alahadeb JIPLoS One10.1371/journal.pone.02662972022Anti-Bacterial Agents/pharmacology, *Escherichia coli/genetics, Humans, Microbial Sensitivity Tests, RNA, Ribosomal, 16S/genetics, Saudi Arabia, Soil, Staphylococcus aureus, *StreptomycesPhylogenyPathogenicity
36294927Evaluation of Green Silver Nanoparticles Fabricated by Spirulina platensis Phycocyanin as Anticancer and Antimicrobial Agents.Soror AS, Ahmed MW, Hassan AEA, Alharbi M, Alsubhi NH, Al-Quwaie DA, Alrefaei GI, Binothman N, Aljadani M, Qahl SH, Jaber FA, Abdalla HLife (Basel)10.3390/life121014932022

Reference

@idauthorscataloguedoi/urltitle
1393Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3463)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3463
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41344Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15225
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
83593Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID4997.1StrainInfo: A central database for resolving microbial strain identifiers
116574Curators of the CIPCollection of Institut Pasteur (CIP 103428)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103428