Strain identifier
BacDive ID: 14450
Type strain:
Species: Staphylococcus aureus
Strain history: CIP <- 1990, ATCC <- FDA, Washington, USA
NCBI tax ID(s): 1280 (species)
General
@ref: 1393
BacDive-ID: 14450
DSM-Number: 3463
keywords: Bacteria, facultative anaerobe, mesophilic, Gram-positive, coccus-shaped, human pathogen
description: Staphylococcus aureus DSM 3463 is a facultative anaerobe, mesophilic, Gram-positive human pathogen of the family Staphylococcaceae.
NCBI tax id
- NCBI tax id: 1280
- Matching level: species
strain history
@ref | history |
---|---|
1393 | <- ATCC; ATCC 29737 <- U.S. Food and Drug Administration (FDA), Washington, D.C., USA; |
116574 | CIP <- 1990, ATCC <- FDA, Washington, USA |
doi: 10.13145/bacdive14450.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Staphylococcaceae
- genus: Staphylococcus
- species: Staphylococcus aureus
- full scientific name: Staphylococcus aureus Rosenbach 1884 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Staphylococcus aureus subsp. anaerobius
@ref: 1393
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Staphylococcaceae
genus: Staphylococcus
species: Staphylococcus aureus
full scientific name: Staphylococcus aureus Rosenbach 1884
type strain: no
Morphology
cell morphology
- @ref: 116574
- gram stain: positive
- cell shape: coccus-shaped
- motility: no
colony morphology
@ref | type of hemolysis | hemolysis ability | incubation period |
---|---|---|---|
1393 | beta | 1 | 1-2 days |
116574 | 1 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
1393 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
1393 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
41344 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
116574 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
1393 | positive | growth | 37 | mesophilic |
41344 | positive | growth | 37 | mesophilic |
116574 | positive | growth | 15-37 | |
116574 | no | growth | 10 | psychrophilic |
116574 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 116574
- oxygen tolerance: facultative anaerobe
antibiogram
@ref | medium | Penicillin G | oxacillin | ampicillin | ticarcillin | mezlocillin | cefalotin | cefazolin | cefotaxime | aztreonam | imipenem | tetracycline | chloramphenicol | gentamycin | amikacin | vancomycin | erythromycin | lincomycin | ofloxacin | norfloxacin | colistin | pipemidic acid | nitrofurantoin | bacitracin | polymyxin b | kanamycin | neomycin | doxycycline | ceftriaxone | clindamycin | fosfomycin | moxifloxacin | linezolid | nystatin | quinupristin/dalfopristin | teicoplanin | piperacillin/tazobactam | incubation temperature |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1393 | Mueller-Hinton Agar | 44-46 | 38 | 42 | 46 | 36 | 44 | 42 | 30 | 0 | 50 | 34 | 32 | 24 | 24 | 22-24 | 30-32 | 30 | 28 | 26 | 0 | 12 | 22 | 18 | 8 | 26 | 24 | 34-36 | 30 | 38 | 40-42 | 34 | 32 | 0 | 28 | 20 | 34 | |
1393 | Mueller-Hinton Agar | 32 | 26 | 30 | 32 | 28 | 26 | 28 | 20 | 0 | 36 | 20 | 20 | 18 | 12 | 10 | 24 | 20 | 20 | 20 | 0 | 10 | 12 | 16 | 8 | 14 | 18 | 20 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | 37 |
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | + | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | + | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | esculin | - | builds acid from | 4853 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | + | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | + | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | + | builds acid from | 17754 |
116574 | nitrate | + | reduction | 17632 |
116574 | nitrite | - | reduction | 16301 |
116574 | tributyrin | - | hydrolysis | 35020 |
68375 | arginine | + | hydrolysis | 29016 |
68375 | ornithine | - | degradation | 18257 |
68375 | esculin | - | hydrolysis | 4853 |
68375 | D-glucose | + | fermentation | 17634 |
68375 | D-fructose | + | fermentation | 15824 |
68375 | D-mannose | + | fermentation | 16024 |
68375 | maltose | + | fermentation | 17306 |
68375 | lactose | + | fermentation | 17716 |
68375 | trehalose | + | fermentation | 27082 |
68375 | D-mannitol | + | fermentation | 16899 |
68375 | raffinose | - | fermentation | 16634 |
68375 | cellobiose | - | fermentation | 17057 |
68375 | nitrate | + | reduction | 17632 |
68375 | sucrose | + | fermentation | 17992 |
68375 | N-acetylglucosamine | + | fermentation | 59640 |
68375 | arabinose | - | fermentation | 22599 |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68375 | 15688 | acetoin | yes |
116574 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test |
---|---|---|---|
68375 | 15688 | acetoin | + |
116574 | 15688 | acetoin | + |
enzymes
@ref | value | activity | ec |
---|---|---|---|
1393 | catalase | + | 1.11.1.6 |
1393 | cytochrome-c oxidase | - | 1.9.3.1 |
68375 | beta-glucuronidase | - | 3.2.1.31 |
68375 | alkaline phosphatase | + | 3.1.3.1 |
68375 | L-arginine arylamidase | - | |
68375 | beta-galactosidase | - | 3.2.1.23 |
68375 | beta-glucosidase | - | 3.2.1.21 |
68375 | ornithine decarboxylase | - | 4.1.1.17 |
68375 | arginine dihydrolase | + | 3.5.3.6 |
116574 | oxidase | - | |
116574 | beta-galactosidase | + | 3.2.1.23 |
116574 | alcohol dehydrogenase | + | 1.1.1.1 |
116574 | gelatinase | +/- | |
116574 | DNase | + | |
116574 | catalase | + | 1.11.1.6 |
116574 | coagulase | + | |
116574 | lecithinase | + | |
116574 | lysine decarboxylase | - | 4.1.1.18 |
116574 | ornithine decarboxylase | - | 4.1.1.17 |
116574 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116574 | - | + | + | + | - | - | - | - | - | - | + | - | - | - | - | + | - | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116574 | + | - | - | - | + | - | - | - | - | + | + | + | + | - | - | - | - | + | - | - | - | + | - | - | - | - | - | + | + | - | + | + | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - |
API ID32STA
@ref | URE | ADH Arg | ODC | ESC | GLU | FRU | MNE | MAL | LAC | TRE | MAN | RAF | RIB | CEL | NIT | VP | beta GAL | ArgA | PAL | PyrA | NOVO | SAC | NAG | TUR | ARA | beta GUR |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1393 | + | + | - | - | + | + | + | + | + | + | + | - | - | - | + | + | - | - | + | - | - | + | + | +/- | - | - |
1393 | - | + | - | - | + | + | + | + | + | + | + | - | + | - | + | + | - | - | + | + | + | + | + | + | - | - |
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
1393 | yes | yes | 2 | Risk group (German classification) |
116574 | 2 | Risk group (French classification) |
External links
@ref: 1393
culture collection no.: DSM 3463, ATCC 29737, CCUG 19207, CIP 103428, NCIMB 12703
straininfo link
- @ref: 83593
- straininfo: 4997
literature
Pubmed-ID | title | authors | journal | DOI | year | mesh | topic | topic2 |
---|---|---|---|---|---|---|---|---|
4042664 | Efficacy of topical chloramphenicol and tobramycin ophthalmic solutions in preventing severe Staphylococcus aureus keratitis in rabbits. | Edwards JG, Schlech BA | Curr Eye Res | 10.3109/02713688509095248 | 1985 | Animals, Chloramphenicol/*administration & dosage/therapeutic use, Keratitis/drug therapy/*etiology, Ophthalmic Solutions, Rabbits, *Staphylococcal Infections, Tobramycin/*administration & dosage/therapeutic use | ||
10861973 | Studies on antibacterial activity of Ficus racemosa Linn. leaf extract. | Mandal SC, Saha BP, Pal M | Phytother Res | 10.1002/1099-1573(200006)14:4<278::aid-ptr592>3.0.co;2-7 | 2000 | Anti-Bacterial Agents/*pharmacology, Bacillus/drug effects, Escherichia coli/drug effects, Microbial Sensitivity Tests, Plant Extracts/*pharmacology, Plant Leaves/chemistry, Plants, Medicinal/*chemistry, Pseudomonas aeruginosa/drug effects, Staphylococcus aureus/drug effects, Trees/*chemistry | Pathogenicity | |
11346296 | Antimicrobial activity in urine: effect on leukocyte count and bacterial culture results. | Abu Shaqra QM | New Microbiol | 2001 | Anti-Bacterial Agents/administration & dosage/pharmacology/*urine, Colony Count, Microbial/*methods, Escherichia coli/drug effects/growth & development, Humans, Leukocyte Count/*methods, Microbial Sensitivity Tests, Reproducibility of Results, Staphylococcus aureus/drug effects/growth & development, Urinary Tract Infections/diagnosis/immunology/microbiology/urine, Urine/chemistry/*cytology/*microbiology | Pathogenicity | ||
17021070 | Genetic background and antibiotic resistance of Staphylococcus aureus strains isolated in the Republic of Georgia. | Revazishvili T, Bakanidze L, Gomelauri T, Zhgenti E, Chanturia G, Kekelidze M, Rajanna C, Kreger A, Sulakvelidze A | J Clin Microbiol | 10.1128/JCM.01030-06 | 2006 | Anti-Bacterial Agents/*pharmacology, *Drug Resistance, Multiple, Bacterial, Georgia (Republic)/epidemiology, Humans, Phylogeny, Staphylococcal Infections/epidemiology/microbiology, Staphylococcus aureus/*drug effects/*genetics | Pathogenicity | Phylogeny |
20235748 | Synthesis of 1-(4-methoxybenzyl)-3-cyclopropyl-1H-pyrazol-5-amine derivatives as antimicrobial agents. | Raju H, Nagamani TS, Chandrappa S, Ananda H, Vinaya K, Thimmegowda NR, Byregowda SM, Rangappa KS | J Enzyme Inhib Med Chem | 10.3109/14756360903357601 | 2010 | Anti-Bacterial Agents, Anti-Infective Agents/*chemical synthesis/chemistry/*pharmacology, Antifungal Agents, Benzamides/*chemical synthesis/pharmacology, Fungi/drug effects, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects, Microbial Sensitivity Tests, Pyrazoles, Structure-Activity Relationship | Pathogenicity | |
23407707 | An Enhancing Effect of Gold Nanoparticles on the Lethal Action of 2450 MHz Electromagnetic Radiation in Microwave Oven. | Mollazadeh-Moghaddam K, Moradi BV, Dolatabadi-Bazaz R, Shakibae M, Shahverdi AR | Avicenna J Med Biotechnol | 2011 | ||||
30719037 | Total Phenolic Content and Antioxidant and Antibacterial Activities of Pereskia bleo. | Johari MA, Khong HY | Adv Pharmacol Sci | 10.1155/2019/7428593 | 2019 | |||
32202302 | Molecular detection of methicillin heat-resistant Staphylococcus aureus strains in pasteurized camel milk in Saudi Arabia. | Aljahani AH, Alarjani KM, Hassan ZK, Elkhadragy MF, Ismail EA, Al-Masoud AH, Yehia HM | Biosci Rep | 10.1042/BSR20193470 | 2020 | Animals, Bacterial Proteins/genetics/isolation & purification, Camelus, Cefoxitin/pharmacology/therapeutic use, DNA, Bacterial/isolation & purification, *Hot Temperature, Humans, Methicillin-Resistant Staphylococcus aureus/drug effects/genetics/*isolation & purification, Microbial Sensitivity Tests/methods, Milk/*microbiology, Pasteurization/*methods, Penicillin-Binding Proteins/genetics/isolation & purification, Polymerase Chain Reaction, Saudi Arabia, Staphylococcal Food Poisoning/drug therapy/microbiology/*prevention & control | Enzymology | Phylogeny |
32359985 | Prevalence of methicillin-resistant (mecA gene) and heat-resistant Staphylococcus aureus strains in pasteurized camel milk. | Yehia HM, Al-Masoud AH, Alarjani KM, Alamri MS | J Dairy Sci | 10.3168/jds.2019-17631 | 2020 | Animals, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/*genetics, Camelus/*microbiology, Cefoxitin/pharmacology, Female, Hot Temperature, Methicillin/pharmacology, *Methicillin Resistance, Methicillin-Resistant Staphylococcus aureus/drug effects/genetics/*isolation & purification/physiology, Milk/*microbiology, Penicillin-Binding Proteins/*genetics, Saudi Arabia, Thermotolerance | Stress | Enzymology |
35421124 | Inhibitory potentials of Streptomyces exfoliatus strain 'MUJA10' against bacterial pathogens isolated from rural areas in Riyadh, Saudi Arabia. | Alahadeb JI | PLoS One | 10.1371/journal.pone.0266297 | 2022 | Anti-Bacterial Agents/pharmacology, *Escherichia coli/genetics, Humans, Microbial Sensitivity Tests, RNA, Ribosomal, 16S/genetics, Saudi Arabia, Soil, Staphylococcus aureus, *Streptomyces | Phylogeny | Pathogenicity |
36294927 | Evaluation of Green Silver Nanoparticles Fabricated by Spirulina platensis Phycocyanin as Anticancer and Antimicrobial Agents. | Soror AS, Ahmed MW, Hassan AEA, Alharbi M, Alsubhi NH, Al-Quwaie DA, Alrefaei GI, Binothman N, Aljadani M, Qahl SH, Jaber FA, Abdalla H | Life (Basel) | 10.3390/life12101493 | 2022 |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
1393 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3463) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3463 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
41344 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15225 | ||
68371 | Automatically annotated from API 50CH acid | |||
68375 | Automatically annotated from API ID32STA | |||
68382 | Automatically annotated from API zym | |||
83593 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID4997.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
116574 | Curators of the CIP | Collection of Institut Pasteur (CIP 103428) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103428 |