Strain identifier
BacDive ID: 14450
Type strain: 
Species: Staphylococcus aureus
Strain history: <- ATCC; ATCC 29737 <- U.S. Food and Drug Administration (FDA), Washington, D.C., USA;
NCBI tax ID(s): 1280 (species)
version 7.1 (current version)
General
@ref: 1393
BacDive-ID: 14450
DSM-Number: 3463
keywords: Bacteria, mesophilic, human pathogen
description: Staphylococcus aureus DSM 3463 is a mesophilic human pathogen of the family Staphylococcaceae.
NCBI tax id
- NCBI tax id: 1280
- Matching level: species
strain history: <- ATCC; ATCC 29737 <- U.S. Food and Drug Administration (FDA), Washington, D.C., USA;
doi: 10.13145/bacdive14450.20221219.7.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Staphylococcaceae
- genus: Staphylococcus
- species: Staphylococcus aureus
- full scientific name: Staphylococcus aureus Rosenbach 1884 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Staphylococcus aureus subsp. anaerobius
@ref: 1393
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Staphylococcaceae
genus: Staphylococcus
species: Staphylococcus aureus
full scientific name: Staphylococcus aureus Rosenbach 1884
type strain: no
Morphology
colony morphology
- @ref: 1393
- type of hemolysis: beta
- incubation period: 1-2 days
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 1393 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
| 1393 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
| 41344 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 1393 | positive | growth | 37 | mesophilic |
| 41344 | positive | growth | 37 | mesophilic |
Physiology and metabolism
antibiogram
| @ref | medium | Penicillin G | oxacillin | ampicillin | ticarcillin | mezlocillin | cefalotin | cefazolin | cefotaxime | aztreonam | imipenem | tetracycline | chloramphenicol | gentamycin | amikacin | vancomycin | erythromycin | lincomycin | ofloxacin | norfloxacin | colistin | pipemidic acid | nitrofurantoin | bacitracin | polymyxin b | kanamycin | neomycin | doxycycline | ceftriaxone | clindamycin | fosfomycin | moxifloxacin | linezolid | nystatin | quinupristin/dalfopristin | teicoplanin | piperacillin/tazobactam | incubation temperature |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1393 | Mueller-Hinton Agar | 44-46 | 38 | 42 | 46 | 36 | 44 | 42 | 30 | 0 | 50 | 34 | 32 | 24 | 24 | 22-24 | 30-32 | 30 | 28 | 26 | 0 | 12 | 22 | 18 | 8 | 26 | 24 | 34-36 | 30 | 38 | 40-42 | 34 | 32 | 0 | 28 | 20 | 34 | |
| 1393 | Mueller-Hinton Agar | 32 | 26 | 30 | 32 | 28 | 26 | 28 | 20 | 0 | 36 | 20 | 20 | 18 | 12 | 10 | 24 | 20 | 20 | 20 | 0 | 10 | 12 | 16 | 8 | 14 | 18 | 20 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | 37 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68375 | 22599 | arabinose | - | fermentation |
| 68375 | 59640 | N-acetylglucosamine | + | fermentation |
| 68375 | 17992 | sucrose | + | fermentation |
| 68375 | 17632 | nitrate | + | reduction |
| 68375 | 17057 | cellobiose | - | fermentation |
| 68375 | 16634 | raffinose | - | fermentation |
| 68375 | 16899 | D-mannitol | + | fermentation |
| 68375 | 27082 | trehalose | + | fermentation |
| 68375 | 17716 | lactose | + | fermentation |
| 68375 | 17306 | maltose | + | fermentation |
| 68375 | 16024 | D-mannose | + | fermentation |
| 68375 | 15824 | D-fructose | + | fermentation |
| 68375 | 17634 | D-glucose | + | fermentation |
| 68375 | 4853 | esculin | - | hydrolysis |
| 68375 | 18257 | ornithine | - | degradation |
| 68375 | 29016 | arginine | + | hydrolysis |
metabolite production
- @ref: 68375
- Chebi-ID: 15688
- metabolite: acetoin
- production: yes
metabolite tests
- @ref: 68375
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: +
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 1393 | catalase | + | 1.11.1.6 |
| 1393 | cytochrome-c oxidase | - | 1.9.3.1 |
| 68375 | beta-glucuronidase | - | 3.2.1.31 |
| 68375 | alkaline phosphatase | + | 3.1.3.1 |
| 68375 | L-arginine arylamidase | - | |
| 68375 | beta-galactosidase | - | 3.2.1.23 |
| 68375 | beta-glucosidase | - | 3.2.1.21 |
| 68375 | ornithine decarboxylase | - | 4.1.1.17 |
| 68375 | arginine dihydrolase | + | 3.5.3.6 |
API ID32STA
| @ref | URE | ADH Arg | ODC | ESC | GLU | FRU | MNE | MAL | LAC | TRE | MAN | RAF | RIB | CEL | NIT | VP | beta GAL | ArgA | PAL | PyrA | NOVO | SAC | NAG | TUR | ARA | beta GUR |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1393 | + | + | - | - | + | + | + | + | + | + | + | - | - | - | + | + | - | - | + | - | - | + | + | +/- | - | - |
| 1393 | - | + | - | - | + | + | + | + | + | + | + | - | + | - | + | + | - | - | + | + | + | + | + | + | - | - |
Safety information
risk assessment
- @ref: 1393
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 1393
culture collection no.: DSM 3463, ATCC 29737, CCUG 19207
straininfo link
| @ref | passport |
|---|---|
| 20218 | http://www.straininfo.net/strains/21819 |
| 20218 | http://www.straininfo.net/strains/21818 |
literature
| Pubmed-ID | title | authors | journal | DOI | year | mesh | topic | topic2 |
|---|---|---|---|---|---|---|---|---|
| 4042664 | Efficacy of topical chloramphenicol and tobramycin ophthalmic solutions in preventing severe Staphylococcus aureus keratitis in rabbits. | Edwards JG, Schlech BA | Curr Eye Res | 10.3109/02713688509095248 | 1985 | Animals, Chloramphenicol/*administration & dosage/therapeutic use, Keratitis/drug therapy/*etiology, Ophthalmic Solutions, Rabbits, *Staphylococcal Infections, Tobramycin/*administration & dosage/therapeutic use | ||
| 10861973 | Studies on antibacterial activity of Ficus racemosa Linn. leaf extract. | Mandal SC, Saha BP, Pal M | Phytother Res | 10.1002/1099-1573(200006)14:4<278::aid-ptr592>3.0.co;2-7 | 2000 | Anti-Bacterial Agents/*pharmacology, Bacillus/drug effects, Escherichia coli/drug effects, Microbial Sensitivity Tests, Plant Extracts/*pharmacology, Plant Leaves/chemistry, Plants, Medicinal/*chemistry, Pseudomonas aeruginosa/drug effects, Staphylococcus aureus/drug effects, Trees/*chemistry | Pathogenicity | |
| 11346296 | Antimicrobial activity in urine: effect on leukocyte count and bacterial culture results. | Abu Shaqra QM | New Microbiol | 2001 | Anti-Bacterial Agents/administration & dosage/pharmacology/*urine, Colony Count, Microbial/*methods, Escherichia coli/drug effects/growth & development, Humans, Leukocyte Count/*methods, Microbial Sensitivity Tests, Reproducibility of Results, Staphylococcus aureus/drug effects/growth & development, Urinary Tract Infections/diagnosis/immunology/microbiology/urine, Urine/chemistry/*cytology/*microbiology | Pathogenicity | ||
| 17021070 | Genetic background and antibiotic resistance of Staphylococcus aureus strains isolated in the Republic of Georgia. | Revazishvili T, Bakanidze L, Gomelauri T, Zhgenti E, Chanturia G, Kekelidze M, Rajanna C, Kreger A, Sulakvelidze A | J Clin Microbiol | 10.1128/JCM.01030-06 | 2006 | Anti-Bacterial Agents/*pharmacology, *Drug Resistance, Multiple, Bacterial, Georgia (Republic)/epidemiology, Humans, Phylogeny, Staphylococcal Infections/epidemiology/microbiology, Staphylococcus aureus/*drug effects/*genetics | Pathogenicity | Phylogeny |
| 20235748 | Synthesis of 1-(4-methoxybenzyl)-3-cyclopropyl-1H-pyrazol-5-amine derivatives as antimicrobial agents. | Raju H, Nagamani TS, Chandrappa S, Ananda H, Vinaya K, Thimmegowda NR, Byregowda SM, Rangappa KS | J Enzyme Inhib Med Chem | 10.3109/14756360903357601 | 2010 | Anti-Bacterial Agents, Anti-Infective Agents/*chemical synthesis/chemistry/*pharmacology, Antifungal Agents, Benzamides/*chemical synthesis/pharmacology, Fungi/drug effects, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects, Microbial Sensitivity Tests, Pyrazoles, Structure-Activity Relationship | Pathogenicity | |
| 23407707 | An Enhancing Effect of Gold Nanoparticles on the Lethal Action of 2450 MHz Electromagnetic Radiation in Microwave Oven. | Mollazadeh-Moghaddam K, Moradi BV, Dolatabadi-Bazaz R, Shakibae M, Shahverdi AR | Avicenna J Med Biotechnol | 2011 | ||||
| 30719037 | Total Phenolic Content and Antioxidant and Antibacterial Activities of Pereskia bleo. | Johari MA, Khong HY | Adv Pharmacol Sci | 10.1155/2019/7428593 | 2019 | |||
| 32202302 | Molecular detection of methicillin heat-resistant Staphylococcus aureus strains in pasteurized camel milk in Saudi Arabia. | Aljahani AH, Alarjani KM, Hassan ZK, Elkhadragy MF, Ismail EA, Al-Masoud AH, Yehia HM | Biosci Rep | 10.1042/BSR20193470 | 2020 | Animals, Bacterial Proteins/genetics/isolation & purification, Camelus, Cefoxitin/pharmacology/therapeutic use, DNA, Bacterial/isolation & purification, *Hot Temperature, Humans, Methicillin-Resistant Staphylococcus aureus/drug effects/genetics/*isolation & purification, Microbial Sensitivity Tests/methods, Milk/*microbiology, Pasteurization/*methods, Penicillin-Binding Proteins/genetics/isolation & purification, Polymerase Chain Reaction, Saudi Arabia, Staphylococcal Food Poisoning/drug therapy/microbiology/*prevention & control | Enzymology | Phylogeny |
| 32359985 | Prevalence of methicillin-resistant (mecA gene) and heat-resistant Staphylococcus aureus strains in pasteurized camel milk. | Yehia HM, Al-Masoud AH, Alarjani KM, Alamri MS | J Dairy Sci | 10.3168/jds.2019-17631 | 2020 | Animals, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/*genetics, Camelus/*microbiology, Cefoxitin/pharmacology, Female, Hot Temperature, Methicillin/pharmacology, *Methicillin Resistance, Methicillin-Resistant Staphylococcus aureus/drug effects/genetics/*isolation & purification/physiology, Milk/*microbiology, Penicillin-Binding Proteins/*genetics, Saudi Arabia, Thermotolerance | Stress | Enzymology |
| 35421124 | Inhibitory potentials of Streptomyces exfoliatus strain 'MUJA10' against bacterial pathogens isolated from rural areas in Riyadh, Saudi Arabia. | Alahadeb JI | PLoS One | 10.1371/journal.pone.0266297 | 2022 | Anti-Bacterial Agents/pharmacology, *Escherichia coli/genetics, Humans, Microbial Sensitivity Tests, RNA, Ribosomal, 16S/genetics, Saudi Arabia, Soil, Staphylococcus aureus, *Streptomyces | Phylogeny | Pathogenicity |
| 36294927 | Evaluation of Green Silver Nanoparticles Fabricated by Spirulina platensis Phycocyanin as Anticancer and Antimicrobial Agents. | Soror AS, Ahmed MW, Hassan AEA, Alharbi M, Alsubhi NH, Al-Quwaie DA, Alrefaei GI, Binothman N, Aljadani M, Qahl SH, Jaber FA, Abdalla H | Life (Basel) | 10.3390/life12101493 | 2022 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1393 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3463) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3463 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 41344 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15225 | ||||
| 68375 | Automatically annotated from API ID32STA |