Strain identifier

BacDive ID: 1444

Type strain: No

Species: Geobacillus stearothermophilus

Strain Designation: 10

Strain history: CIP <- 2001, DSMZ <- J. Stenesh

NCBI tax ID(s): 1891658 (species)

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General

@ref: 4979

BacDive-ID: 1444

DSM-Number: 13240

keywords: genome sequence, Bacteria, obligate aerobe, spore-forming, thermophilic, motile

description: Geobacillus stearothermophilus 10 is an obligate aerobe, spore-forming, thermophilic bacterium that was isolated from hot springs.

NCBI tax id

  • NCBI tax id: 1891658
  • Matching level: species

strain history

@refhistory
4979<- J. Stenesh
123298CIP <- 2001, DSMZ <- J. Stenesh

doi: 10.13145/bacdive1444.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Geobacillus
  • species: Geobacillus stearothermophilus
  • full scientific name: Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001

  • synonyms

    • @ref: 20215
    • synonym: Bacillus stearothermophilus

@ref: 4979

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Geobacillus

species: Geobacillus stearothermophilus

full scientific name: Geobacillus sp.

strain designation: 10

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes94.271
69480100positive
123298nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4979CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Yeast extract 50.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
4979TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34094MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123298CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerangeconfidence
4979positivegrowth55thermophilic
34094positivegrowth55thermophilic
69480thermophilic100
123298positivegrowth45-55thermophilic
123298nogrowth10psychrophilic
123298nogrowth22psychrophilic
123298nogrowth30mesophilic
123298nogrowth37mesophilic

culture pH

  • @ref: 123298
  • ability: no
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 123298
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes99.999
123298yes

halophily

@refsaltgrowthtested relationconcentration
123298NaClpositivegrowth0-2 %
123298NaClnogrowth4 %
123298NaClnogrowth6 %
123298NaClnogrowth8 %
123298NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371inulin-builds acid from15443
68371cellobiose-builds acid from17057
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123298citrate-carbon source16947
123298esculin+hydrolysis4853
123298hippurate+hydrolysis606565
123298nitrate-reduction17632
123298nitrite-reduction16301
123298nitrate-respiration17632

metabolite production

  • @ref: 123298
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12329815688acetoin-
12329817234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
123298oxidase+
123298beta-galactosidase-3.2.1.23
123298alcohol dehydrogenase+1.1.1.1
123298gelatinase+/-
123298amylase+
123298catalase+1.11.1.6
123298tween esterase-
123298gamma-glutamyltransferase+2.3.2.2
123298lysine decarboxylase-4.1.1.18
123298ornithine decarboxylase-4.1.1.17
123298urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123298--++-+---+++++------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123298----+/-+/----+/-+/-+/-+/-----------+/-+/-+/--+/-+/-+/-+/-+/--+/-+/-+/-+/---+/----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4979hot springsMontana, Yellow Stone National ParkUSAUSANorth America
123298Environment, Hot springsYellowstone, MontanaUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 123298
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Geobacillus stearothermophilus 10272567.8completepatric272567
66792Geobacillus stearothermophilus 10272567.18plasmidpatric272567

GC content

  • @ref: 4979
  • GC-content: 53

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes90.421no
gram-positiveyes83.886no
anaerobicno97.935no
halophileno96.2no
spore-formingyes96.213no
glucose-utilyes91.053no
aerobicyes87.187no
flagellatedyes87.035no
thermophileyes99.999yes
glucose-fermentno89.38no

External links

@ref: 4979

culture collection no.: DSM 13240, CIP 106956

straininfo link

  • @ref: 71094
  • straininfo: 307563

Reference

@idauthorscataloguedoi/urltitle
4979Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13240)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13240
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34094Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19143
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71094Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID307563.1StrainInfo: A central database for resolving microbial strain identifiers
123298Curators of the CIPCollection of Institut Pasteur (CIP 106956)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106956