Strain identifier

BacDive ID: 14424

Type strain: Yes

Species: Macrococcus hajekii

Strain history: CIP <- 2003, CCM

NCBI tax ID(s): 198482 (species)

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General

@ref: 8070

BacDive-ID: 14424

DSM-Number: 19357

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Macrococcus hajekii DSM 19357 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from llama skin.

NCBI tax id

  • NCBI tax id: 198482
  • Matching level: species

strain history

@refhistory
8070<- CCM, CCM 4809
339032003, CCM
119788CIP <- 2003, CCM

doi: 10.13145/bacdive14424.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Macrococcus
  • species: Macrococcus hajekii
  • full scientific name: Macrococcus hajekii Mannerová et al. 2003

@ref: 8070

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Macrococcus

species: Macrococcus hajekii

full scientific name: Macrococcus hajekii Mannerová et al. 2003

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
119788positivecoccus-shapedno

colony morphology

  • @ref: 119788

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8070TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
33903MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
119788CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
119788CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
8070positivegrowth35mesophilic
33903positivegrowth37mesophilic
57576positivegrowth25-37mesophilic
119788positivegrowth22-37
119788nogrowth10psychrophilic
119788nogrowth41thermophilic
119788nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57576aerobe
119788obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.775

halophily

@refsaltgrowthtested relationconcentration
119788NaClnogrowth0 %
119788NaClnogrowth2 %
119788NaClnogrowth4 %
119788NaClnogrowth6 %
119788NaClnogrowth8 %
119788NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
119788606565hippurate+hydrolysis
11978817632nitrate+reduction
11978816301nitrite-reduction
6837516199urea-hydrolysis
6837529016arginine-hydrolysis
6837518257ornithine-degradation
683754853esculin-hydrolysis
6837517634D-glucose-fermentation
6837516024D-mannose-fermentation
6837517306maltose-fermentation
6837517716lactose-fermentation
6837527082trehalose-fermentation
6837516899D-mannitol-fermentation
6837516634raffinose-fermentation
6837516988D-ribose-fermentation
6837517057cellobiose-fermentation
6837517632nitrate+reduction
6837517992sucrose-fermentation
6837559640N-acetylglucosamine-fermentation
6837532528turanose-fermentation
6837522599arabinose-fermentation

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is sensitive: yes
  • sensitivity conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinno
11978835581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6837515688acetoin-
11978815688acetoin-

enzymes

@refvalueactivityec
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase-3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5
119788oxidase-
119788beta-galactosidase-3.2.1.23
119788alcohol dehydrogenase-1.1.1.1
119788gelatinase-
119788amylase-
119788DNase-
119788caseinase-3.4.21.50
119788catalase+1.11.1.6
119788coagulase-
119788tween esterase-
119788gamma-glutamyltransferase-2.3.2.2
119788lecithinase-
119788lipase-
119788lysine decarboxylase-4.1.1.18
119788ornithine decarboxylase-4.1.1.17
119788phenylalanine ammonia-lyase-4.3.1.24
119788protease-
119788urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119788--+-------++--------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8070-----+/---------+-----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
8070llama skinBrno, Zoological GardenCzech RepublicCZEEurope
57576Llama skin (Lama glama)Brno,Brno ZooCzech RepublicCZEEurope
119788Skin of llamas, Lama glama L.BrnoCzech RepublicCZEEurope1997

isolation source categories

Cat1Cat2Cat3
#Host#Mammals
#Host Body-Site#Organ#Skin, Nail, Hair

taxonmaps

  • @ref: 69479
  • File name: preview.99_26367.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1248;97_1481;98_6269;99_26367&stattab=map
  • Last taxonomy: Macrococcus
  • 16S sequence: AY119685
  • Sequence Identity:
  • Total samples: 2524
  • soil counts: 184
  • aquatic counts: 145
  • animal counts: 2112
  • plant counts: 83

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
80701Risk group (German classification)
1197881Risk group (French classification)

Sequence information

16S sequences

  • @ref: 8070
  • description: Macrococcus hajekii 16S ribosomal RNA gene, complete sequence
  • accession: AY119685
  • length: 1548
  • database: ena
  • NCBI tax ID: 198482

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Macrococcus hajekii CCM 4809GCA_014634985contigncbi198482
66792Macrococcus hajekii CCM4809GCA_004359505contigncbi198482
66792Macrococcus hajekii strain CCM 4809198482.4wgspatric198482
66792Macrococcus hajekii strain CCM4809198482.3wgspatric198482
66792Macrococcus hajekii CCM48092898324690draftimg198482

GC content

  • @ref: 8070
  • GC-content: 40

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno59no
motileno92.047no
gram-positiveyes92.992no
anaerobicno98.731no
aerobicyes89.105no
halophileyes88.856no
spore-formingno83.114no
glucose-utilyes87.245no
flagellatedno96.127no
thermophileno99.821no
glucose-fermentno77.53yes

External links

@ref: 8070

culture collection no.: DSM 19357, CCM 4809, CCUG 47201, CIP 108166, LMG 21711

straininfo link

  • @ref: 83568
  • straininfo: 87782

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130064Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas.Mannerova S, Pantucek R, Doskar J, Svec P, Snauwaert C, Vancanneyt M, Swings J, Sedlacek IInt J Syst Evol Microbiol10.1099/ijs.0.02683-02003Animals, Base Composition, Base Sequence, Camelids, New World/*microbiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Skin/microbiology, Staphylococcaceae/*classification/genetics/*isolation & purification/metabolismGenetics
Genetics31072882Draft Genome Sequences of the Type Strains of Six Macrococcus Species.Mazhar S, Altermann E, Hill C, McAuliffe OMicrobiol Resour Announc10.1128/MRA.00344-192019

Reference

@idauthorscataloguedoi/urltitle
8070Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19357)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19357
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33903Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5758
57576Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47201)https://www.ccug.se/strain?id=47201
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
83568Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID87782.1StrainInfo: A central database for resolving microbial strain identifiers
119788Curators of the CIPCollection of Institut Pasteur (CIP 108166)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108166