Strain identifier

BacDive ID: 14401

Type strain: Yes

Species: Sporolactobacillus nakayamae subsp. racemicus

Strain Designation: M-17

Strain history: CIP <- 1997, F. Yanagida, Yamanashi Univ., Japan: strain M-17 <- O. Nakayama

NCBI tax ID(s): 33027 (subspecies)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.2 (current version):
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9.2 (current version)

General

@ref: 6366

BacDive-ID: 14401

DSM-Number: 16324

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, motile, rod-shaped

description: Sporolactobacillus nakayamae subsp. racemicus M-17 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 33027
  • Matching level: subspecies

strain history

@refhistory
6366<- JCM <- F. Yanagida <- IAM <- O. Nakayama; M-17
67770F. Yanagida <-- IAM 12396 <-- O. Nakayama M-17.
121224CIP <- 1997, F. Yanagida, Yamanashi Univ., Japan: strain M-17 <- O. Nakayama

doi: 10.13145/bacdive14401.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Sporolactobacillaceae
  • genus: Sporolactobacillus
  • species: Sporolactobacillus nakayamae subsp. racemicus
  • full scientific name: Sporolactobacillus nakayamae subsp. racemicus Yanagida et al. 1997

  • synonyms

    • @ref: 20215
    • synonym: Sporolactobacillus racemicus

@ref: 6366

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Sporolactobacillaceae

genus: Sporolactobacillus

species: Sporolactobacillus nakayamae subsp. racemicus

full scientific name: Sporolactobacillus nakayamae subsp. racemicus Yanagida et al. 1997

strain designation: M-17

type strain: yes

Morphology

cell morphology

  • @ref: 121224
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 121224

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6366GYP GLUCOSE-YEAST-PEPTONE MEDIUM (DSMZ Medium 852)yeshttps://mediadive.dsmz.de/medium/852Name: GYP GLUCOSE-YEAST-PEPTONE MEDIUM (DSMZ Medium 852) Composition: Glucose 20.0 g/l Yeast extract 10.0 g/l Peptone 10.0 g/l Na-acetate 10.0 g/l Agar 10.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x 4 H2O 0.01 g/l FeSO4 x 7 H2O 0.01 g/l NaCl 0.01 g/l Distilled water
39262MEDIUM 217 - for SporolactobacillusyesDistilled water make up to (1000.000 ml);Sodium chloride (0.100 g);Manganese II sulphate monohydrate (0.160 g);Magnesium sulphate heptahydrate (2.000 g);Glucose (20.000 g);Yeast extract (10.000 g);Peptone (10.000 g);Sodium acetate (10.000 g);Iron (III) su
6366R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
121224CIP Medium 260yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=260
121224CIP Medium 217yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=217

culture temp

@refgrowthtypetemperature
6366positivegrowth30
39262positivegrowth30
67770positivegrowth30
121224positivegrowth15
121224nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6366microaerophile
121224facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
121224NaClpositivegrowth0-6 %
121224NaClnogrowth8 %
121224NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1212244853esculin+hydrolysis
12122417632nitrate-reduction
12122416301nitrite-reduction
68371Potassium 5-ketogluconate-builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose+builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837128053melibiose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from

enzymes

@refvalueactivityec
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382alkaline phosphatase-3.1.3.1
121224oxidase-
121224beta-galactosidase-3.2.1.23
121224gelatinase-
121224amylase-
121224DNase-
121224caseinase-3.4.21.50
121224catalase-1.11.1.6
121224tween esterase-
121224lecithinase-
121224lipase-
121224urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-galactosidase-3.2.1.22
68382alpha-chymotrypsin-3.4.21.1

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121224-----+++---+-+------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121224+--+++---++++--------++++++++/--+/-+---+/-+/--++-+-------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinenthost species
6366soilJapanJPNAsia
55163Soil
67770Rhizosphere of starwort (Aster indicus)Aster indicus
121224Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
63661Risk group (German classification)
1212241Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sporolactobacillus nakayamae subsp. racemicus gene for 16S rRNA, partial sequence, strain: NRIC 0349 (= ATCC 700381, = JCM 3417)AB3626361543nuccore33027
20218Sporolactobacillus nakayamae 16S rRNA gene, type strain DSM 16324TAJ6988601504nuccore269670
20218Sporolactobacillus nakayamae subsp. racemicus gene for 16S rRNA, partial sequence, strain: IAM 12396D162911483nuccore33027
20218Sporolactobacillus nakayamae subsp. racemicus gene for 16S ribosomal RNA, partial sequenceAB3745191501nuccore33027
20218Sporolactobacillus nakayamae subsp. racemicus gene for 16S rRNA, partial sequence, strain: NBRC 101524AB6814661475nuccore33027

GC content

  • @ref: 67770
  • GC-content: 45.8
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 6366

culture collection no.: DSM 16324, ATCC 700381, CCUG 41659, CIP 105318, IAM 12396, JCM 3417, LMG 18785, CGMCC 1.3843, NBRC 101524

straininfo link

  • @ref: 83545
  • straininfo: 12434

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6366Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16324)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16324
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39262Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17325
55163Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 41659)https://www.ccug.se/strain?id=41659
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
83545Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12434.1StrainInfo: A central database for resolving microbial strain identifiers
121224Curators of the CIPCollection of Institut Pasteur (CIP 105318)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105318