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Strain identifier

BacDive ID: 14385

Type strain: Yes

Species: Sporolactobacillus laevolacticus

Strain Designation: M-8

Strain history: IAM 12321 <-- O. Nakayama M-8.

NCBI tax ID(s): 1395513 (strain), 33018 (species)

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General

@ref: 208

BacDive-ID: 14385

DSM-Number: 442

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, lactate production

description: Sporolactobacillus laevolacticus M-8 is an anaerobe, mesophilic bacterium that produces lactate and was isolated from rhizosphere of ditch crowfoot.

NCBI tax id

NCBI tax idMatching level
1395513strain
33018species

strain history

doi: 10.13145/bacdive14385.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Sporolactobacillaceae
  • genus: Sporolactobacillus
  • species: Sporolactobacillus laevolacticus
  • full scientific name: Sporolactobacillus laevolacticus (Andersch et al. 1994) Hatayama et al. 2006

  • synonyms

    • @ref: 20215
    • synonym: Bacillus laevolacticus

@ref: 208

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Sporolactobacillaceae

genus: Sporolactobacillus

species: Sporolactobacillus laevolacticus

full scientific name: Sporolactobacillus laevolacticus (Andersch et al. 1994) Hatayama et al. 2006

strain designation: M-8

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 95.838

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
208CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220.pdf
41166MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinusyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g)
208BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8)yeshttps://mediadive.dsmz.de/medium/8Name: BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Yeast extract 5.0 g/l Peptone 5.0 g/l Glucose 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
208positivegrowth30mesophilic
41166positivegrowth30mesophilic
51643positivegrowth37mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 51643
  • oxygen tolerance: anaerobe

spore formation

  • @ref: 69480
  • spore formation: yes
  • confidence: 92.86

compound production

  • @ref: 208
  • compound: D(-) lactic acid

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 80.94

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838116199urea-hydrolysis
6838116443D-tagatose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
683816731melezitose-builds acid from
6838128053melibiose-builds acid from
6838117306maltose-builds acid from
6838127941pullulan-builds acid from
6838128087glycogen-builds acid from
68381606565hippurate-hydrolysis
6838140585alpha-cyclodextrin-builds acid from
6838118333D-arabitol-builds acid from
6838130849L-arabinose-builds acid from
6838117992sucrose-builds acid from
6838116634raffinose-builds acid from
6838127082trehalose-builds acid from
6838117716lactose-builds acid from
6838130911sorbitol-builds acid from
6838116899D-mannitol-builds acid from
6838116988D-ribose-builds acid from
6838129016arginine-hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose+builds acid from
6837162318D-lyxose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
683716731melezitose+builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose-fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6838115688acetoinyes
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6838115688acetoin+
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARA
51643--------------------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
51643----------++++--------------+-+++-+-------+-------
51643----------+++++---+---+-----++++++++----+-+-------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
51643----+-----------+---------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinenthost species
208rhizosphere of ditch crowfootKofuJapanJPNAsia
51643Rhizosphere of ditch crowfoot
67770Rhizosphere of ditch crowfoot (Ranunculus sceleratus)Ranunculus sceleratus

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

taxonmaps

  • @ref: 69479
  • File name: preview.99_7487.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_414;96_3495;97_4284;98_5485;99_7487&stattab=map
  • Last taxonomy: Sporolactobacillus
  • 16S sequence: AB374516
  • Sequence Identity:
  • Total samples: 1822
  • soil counts: 181
  • aquatic counts: 140
  • animal counts: 1421
  • plant counts: 80

Safety information

risk assessment

  • @ref: 208
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sporolactobacillus laevolacticus gene for 16S rRNA, partial sequence, strain: NRIC 0361 (= ATCC 23492, = JCM 2513)AB3626421545ena33018
20218Bacillus laevolacticus clone GN-1_F 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF478072522ena33018
20218Sporolactobacillus laevolacticus gene for 16S ribosomal RNA, partial sequenceAB3745161503ena33018
20218Sporolactobacillus laevolacticus gene for 16S rRNA, strain: IAM 12321D162701484ena33018
20218Sporolactobacillus laevolacticus gene for 16S rRNA, partial sequenceAB3716361476ena33018

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sporolactobacillus laevolacticus DSM 4421395513.3wgspatric1395513
66792Sporolactobacillus laevolacticus DSM 4422554235469draftimg1395513
67770Sporolactobacillus laevolacticus DSM 442 DSM 442GCA_000497245contigncbi1395513

GC content

@refGC-contentmethod
20841.5
6777044.7thermal denaturation, midpoint method (Tm)
6777043thermal denaturation, midpoint method (Tm)

External links

@ref: 208

culture collection no.: DSM 442, ATCC 23492, NCIB 10269, CCUG 33623, LMG 16675, CIP 104421, IAM 12321, JCM 2513, LMG 6329, BCRC 11598, CECT 692, KCTC 3117, NBRC 102473, NCIMB 10269

straininfo link

@refpassport
20218http://www.straininfo.net/strains/5031
20218http://www.straininfo.net/strains/5027
20218http://www.straininfo.net/strains/123470

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Biotechnology23735801Efficient production of polymer-grade D-lactate by Sporolactobacillus laevolacticus DSM442 with agricultural waste cottonseed as the sole nitrogen source.Li Y, Wang L, Ju J, Yu B, Ma YBioresour Technol10.1016/j.biortech.2013.04.1242013*Cotton Fiber, Glucose/analysis, Lactic Acid/*chemistry, Lactobacillus/*metabolism, Nitrogen/*chemistry, TemperatureMetabolism
Genetics24371202Genome Sequence of Sporolactobacillus laevolacticus DSM442, an Efficient Polymer-Grade D-Lactate Producer from Agricultural Waste Cottonseed as a Nitrogen Source.Wang H, Wang L, Ju J, Yu B, Ma YGenome Announc10.1128/genomeA.01100-132013

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
208Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 442)https://www.dsmz.de/collection/catalogue/details/culture/DSM-442
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41166Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16328
51643Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33623)https://www.ccug.se/strain?id=33623
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)