Strain identifier

BacDive ID: 14385

Type strain: Yes

Species: Sporolactobacillus laevolacticus

Strain Designation: M-8

Strain history: CIP <- 1995, JCM <- IAM <- O. Nakamaya: strain M-8

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 208

BacDive-ID: 14385

DSM-Number: 442

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, motile, lactate production

description: Sporolactobacillus laevolacticus M-8 is an anaerobe, spore-forming, mesophilic bacterium that produces lactate and was isolated from rhizosphere of ditch crowfoot.

NCBI tax id

NCBI tax idMatching level
1395513strain
33018species

strain history

@refhistory
208<- O. Nakayama, M-8
67770IAM 12321 <-- O. Nakayama M-8.
120546CIP <- 1995, JCM <- IAM <- O. Nakamaya: strain M-8

doi: 10.13145/bacdive14385.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Sporolactobacillaceae
  • genus: Sporolactobacillus
  • species: Sporolactobacillus laevolacticus
  • full scientific name: Sporolactobacillus laevolacticus (Andersch et al. 1994 ex Nakayama and Yanoshi 1967) Hatayama et al. 2006
  • synonyms

    • @ref: 20215
    • synonym: Bacillus laevolacticus

@ref: 208

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Sporolactobacillaceae

genus: Sporolactobacillus

species: Sporolactobacillus laevolacticus

full scientific name: Sporolactobacillus laevolacticus (Andersch et al. 1994) Hatayama et al. 2006

strain designation: M-8

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes91.423
69480100positive
120546yespositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
208CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220.pdf
41166MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinusyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g)
208BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8)yeshttps://mediadive.dsmz.de/medium/8Name: BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Yeast extract 5.0 g/l Peptone 5.0 g/l Glucose 5.0 g/l Distilled water
120546CIP Medium 41yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41

culture temp

@refgrowthtypetemperaturerange
208positivegrowth30mesophilic
41166positivegrowth30mesophilic
51643positivegrowth37mesophilic
67770positivegrowth30mesophilic
120546positivegrowth18-45
120546nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
51643anaerobe
120546facultative anaerobe

spore formation

@refspore formationconfidence
69481yes96
69480yes100
120546yes

compound production

  • @ref: 208
  • compound: D(-) lactic acid

halophily

@refsaltgrowthtested relationconcentration
120546NaClpositivegrowth0-2 %
120546NaClnogrowth4 %
120546NaClnogrowth6 %
120546NaClnogrowth8 %
120546NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371melezitose+builds acid from6731
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68368L-arabinose-fermentation30849
68368amygdalin-fermentation27613
68368melibiose-fermentation28053
68368sucrose-fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol-fermentation16899
68368D-glucose-fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016
120546nitrate-reduction17632
120546nitrite-reduction16301
120546nitrate+respiration17632
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose-builds acid from17716
68381trehalose-builds acid from27082
68381raffinose-builds acid from16634
68381sucrose-builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose-builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
12054635581indoleno
6838115688acetoinyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6838115688acetoin+
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
120546alcohol dehydrogenase-1.1.1.1
120546amylase-
120546catalase+1.11.1.6
120546lysine decarboxylase-4.1.1.18
120546ornithine decarboxylase-4.1.1.17
120546urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120546----------++--------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARA
51643--------------------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
51643----------++++--------------+-+++-+-------+-------
51643----------+++++---+---+-----++++++++----+-+-------
120546---------+++++---++--+-----++/-++++++------+-------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
51643----+-----------+---------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120546+++++-+++----------------+-------+--+--------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinenthost species
208rhizosphere of ditch crowfootKofuJapanJPNAsia
51643Rhizosphere of ditch crowfoot
67770Rhizosphere of ditch crowfoot (Ranunculus sceleratus)Ranunculus sceleratus
120546Environment, Ditch crowfoot, rhizosphere: Ranunculus

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

taxonmaps

  • @ref: 69479
  • File name: preview.99_7487.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_414;96_3495;97_4284;98_5485;99_7487&stattab=map
  • Last taxonomy: Sporolactobacillus
  • 16S sequence: AB374516
  • Sequence Identity:
  • Total samples: 1822
  • soil counts: 181
  • aquatic counts: 140
  • animal counts: 1421
  • plant counts: 80

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
2081Risk group (German classification)
1205461Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sporolactobacillus laevolacticus gene for 16S rRNA, partial sequence, strain: NRIC 0361 (= ATCC 23492, = JCM 2513)AB3626421545ena33018
20218Bacillus laevolacticus clone GN-1_F 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF478072522ena33018
20218Sporolactobacillus laevolacticus gene for 16S ribosomal RNA, partial sequenceAB3745161503ena33018
20218Sporolactobacillus laevolacticus gene for 16S rRNA, strain: IAM 12321D162701484ena33018
20218Sporolactobacillus laevolacticus gene for 16S rRNA, partial sequenceAB3716361476ena33018

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sporolactobacillus laevolacticus DSM 4421395513.3wgspatric1395513
66792Sporolactobacillus laevolacticus DSM 4422554235469draftimg1395513
67770Sporolactobacillus laevolacticus DSM 442GCA_000497245contigncbi1395513

GC content

@refGC-contentmethod
20841.5
6777044.7thermal denaturation, midpoint method (Tm)
6777043thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes96no
motileyes89.422no
gram-positiveyes82.285no
anaerobicno87.306yes
halophileno73.226no
spore-formingyes95.216no
thermophileno98.831no
glucose-utilyes83.853no
aerobicno92.796yes
flagellatedyes78.599no
glucose-fermentno75.331yes

External links

@ref: 208

culture collection no.: DSM 442, ATCC 23492, NCIB 10269, CCUG 33623, LMG 16675, CIP 104421, IAM 12321, JCM 2513, LMG 6329, BCRC 11598, CECT 692, KCTC 3117, NBRC 102473, NCIMB 10269

straininfo link

  • @ref: 83528
  • straininfo: 92157

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Biotechnology23735801Efficient production of polymer-grade D-lactate by Sporolactobacillus laevolacticus DSM442 with agricultural waste cottonseed as the sole nitrogen source.Li Y, Wang L, Ju J, Yu B, Ma YBioresour Technol10.1016/j.biortech.2013.04.1242013*Cotton Fiber, Glucose/analysis, Lactic Acid/*chemistry, Lactobacillus/*metabolism, Nitrogen/*chemistry, TemperatureMetabolism
Genetics24371202Genome Sequence of Sporolactobacillus laevolacticus DSM442, an Efficient Polymer-Grade D-Lactate Producer from Agricultural Waste Cottonseed as a Nitrogen Source.Wang H, Wang L, Ju J, Yu B, Ma YGenome Announc10.1128/genomeA.01100-132013

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
208Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 442)https://www.dsmz.de/collection/catalogue/details/culture/DSM-442
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41166Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16328
51643Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33623)https://www.ccug.se/strain?id=33623
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83528Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92157.1StrainInfo: A central database for resolving microbial strain identifiers
120546Curators of the CIPCollection of Institut Pasteur (CIP 104421)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104421