Strain identifier
BacDive ID: 14357
Type strain: 
Species: Spiroplasma melliferum
Strain history: <- J. K. Davis {2008} <- J. G. Tully <- R. F. Whitcomb <- T. B. Clark
NCBI tax ID(s): 2134 (species)
version 8.1 (current version)
General
@ref: 15979
BacDive-ID: 14357
DSM-Number: 21833
keywords: 16S sequence, Bacteria, microaerophile, mesophilic, animal pathogen
description: Spiroplasma melliferum DSM 21833 is a microaerophile, mesophilic animal pathogen that was isolated from honeybee, Apis mellifera.
NCBI tax id
- NCBI tax id: 2134
- Matching level: species
strain history
- @ref: 15979
- history: <- J. K. Davis {2008} <- J. G. Tully <- R. F. Whitcomb <- T. B. Clark
doi: 10.13145/bacdive14357.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/mycoplasmatota
- domain: Bacteria
- phylum: Mycoplasmatota
- class: Mollicutes
- order: Mycoplasmatales
- family: Spiroplasmataceae
- genus: Spiroplasma
- species: Spiroplasma melliferum
- full scientific name: Spiroplasma melliferum Clark et al. 1985
@ref: 15979
domain: Bacteria
phylum: Tenericutes
class: Mollicutes
order: Entomoplasmatales
family: Spiroplasmataceae
genus: Spiroplasma
species: Spiroplasma melliferum
full scientific name: Spiroplasma melliferum Clark et al. 1985
type strain: yes
Morphology
colony morphology
- @ref: 15979
- incubation period: 1-2 days
Culture and growth conditions
culture medium
- @ref: 15979
- name: SP4-Z MEDIUM (DSMZ Medium 1076b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1076b
- composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water
culture temp
- @ref: 15979
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 15979
- oxygen tolerance: microaerophile
Isolation, sampling and environmental information
isolation
- @ref: 15979
- sample type: honeybee, Apis mellifera
- host species: Apis mellifera
- geographic location: Maryland
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
- Cat1: #Host
- Cat2: #Arthropoda
- Cat3: #Insecta
taxonmaps
- @ref: 69479
- File name: preview.99_1350.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15899;96_780;97_909;98_1066;99_1350&stattab=map
- Last taxonomy: Spiroplasma
- 16S sequence: AY325304
- Sequence Identity:
- Total samples: 454
- soil counts: 4
- aquatic counts: 24
- animal counts: 424
- plant counts: 2
Safety information
risk assessment
- @ref: 15979
- pathogenicity animal: yes
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15979
- description: Spiroplasma melliferum strain BC-3 16S ribosomal RNA gene, partial sequence
- accession: AY325304
- length: 1515
- database: ena
- NCBI tax ID: 2134
GC content
| @ref | GC-content | method |
|---|---|---|
| 15979 | 27-28 | Buoyant density centrifugation (BD) |
| 15979 | 26 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 15979
culture collection no.: DSM 21833, ATCC 33219, BC 3
straininfo link
- @ref: 83500
- straininfo: 40399
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 2332410 | A 57-kilodalton protein associated with Spiroplasma melliferum fibrils undergoes reversible phosphorylation. | Platt MW, Reizer J, Rottem S | J Bacteriol | 10.1128/jb.172.5.2808-2811.1990 | 1990 | Bacterial Proteins/isolation & purification/*metabolism, Cell Movement, Molecular Weight, Organelles/metabolism, Phosphoproteins/isolation & purification, Phosphorylation, Spiroplasma/*metabolism/physiology | Enzymology |
| Genetics | 3117724 | Organization and structure of tRNA genes in Spiroplasma melliferum. | Rogers MJ, Steinmetz AA, Walker RT | Isr J Med Sci | 1987 | Bacillus subtilis/genetics, Base Sequence, Genes, *Genes, Bacterial, Molecular Sequence Data, Mycoplasma mycoides/genetics, RNA, Transfer/*genetics, Sequence Homology, Nucleic Acid, Species Specificity, Spiroplasma/*genetics | Phylogeny | |
| Genetics | 7756323 | Solution structure determination by NMR spectroscopy of a synthetic peptide corresponding to a putative amphipathic alpha-helix of spiralin: resonance assignment, distance geometry and simulated annealing. | Bondon A, Berthault P, Segalas I, Perly B, Wroblewski H | Biochim Biophys Acta | 10.1016/0005-2736(95)80002-w | 1995 | Amino Acid Sequence, Bacterial Outer Membrane Proteins/*chemistry, *Magnetic Resonance Spectroscopy, Models, Molecular, Molecular Sequence Data, Peptide Fragments/*chemistry, *Protein Structure, Secondary, Solutions, Spiroplasma/chemistry | |
| 11532147 | A bacterial linear motor: cellular and molecular organization of the contractile cytoskeleton of the helical bacterium Spiroplasma melliferum BC3. | Trachtenberg S, Gilad R | Mol Microbiol | 10.1046/j.1365-2958.2001.02527.x | 2001 | Bacterial Proteins/chemistry/physiology/ultrastructure, Cryoelectron Microscopy/methods, Cytoskeleton/*chemistry/*ultrastructure, Models, Molecular, Molecular Motor Proteins/*chemistry/physiology/*ultrastructure, Molecular Weight, Movement, Phosphorylation, Protein Subunits, Spiroplasma/*chemistry/*cytology/ultrastructure | ||
| Cultivation | 12535068 | Motility modes of Spiroplasma melliferum BC3: a helical, wall-less bacterium driven by a linear motor. | Gilad R, Porat A, Trachtenberg S | Mol Microbiol | 10.1046/j.1365-2958.2003.03200.x | 2003 | Culture Media, Image Processing, Computer-Assisted/methods, Microscopy/methods, Movement, Optics and Photonics/instrumentation, Spiroplasma/growth & development/*physiology/*ultrastructure, Viscosity | |
| Cultivation | 12535069 | The bacterial linear motor of Spiroplasma melliferum BC3: from single molecules to swimming cells. | Trachtenberg S, Gilad R, Geffen N | Mol Microbiol | 10.1046/j.1365-2958.2003.t01-1-03200.x | 2003 | Chemotaxis, Culture Media, Cytoskeleton/*metabolism, Image Processing, Computer-Assisted, Microscopy/methods, Microscopy, Electron, Models, Biological, Molecular Motor Proteins/*metabolism, Movement, Spiroplasma/growth & development/*physiology/*ultrastructure | Metabolism |
| Metabolism | 18400234 | Structure of the cytoskeleton of Spiroplasma melliferum BC3 and its interactions with the cell membrane. | Trachtenberg S, Dorward LM, Speransky VV, Jaffe H, Andrews SB, Leapman RD | J Mol Biol | 10.1016/j.jmb.2008.02.020 | 2008 | Cell Membrane/metabolism/*ultrastructure, Cryopreservation, Cytoskeleton/metabolism/*ultrastructure, DNA, Bacterial/genetics, Electrophoresis, Gel, Two-Dimensional, Microscopy, Electron, Models, Molecular, Proteomics, Spiroplasma/genetics/metabolism/*ultrastructure | Enzymology |
| Enzymology | 21605565 | The monomeric, tetrameric, and fibrillar organization of Fib: the dynamic building block of the bacterial linear motor of Spiroplasma melliferum BC3. | Cohen-Krausz S, Cabahug PC, Trachtenberg S | J Mol Biol | 10.1016/j.jmb.2011.04.067 | 2011 | Bacterial Proteins/*chemistry/ultrastructure, Cytoskeletal Proteins/*chemistry/ultrastructure, Models, Molecular, Molecular Dynamics Simulation, Molecular Motor Proteins/*chemistry/ultrastructure, Pentosyltransferases/chemistry/ultrastructure, Phosphorylation, Protein Structure, Secondary, Protein Structure, Tertiary, Spiroplasma/*enzymology/ultrastructure | |
| Metabolism | 24586297 | A structural framework for a near-minimal form of life: mass and compositional analysis of the helical mollicute Spiroplasma melliferum BC3. | Trachtenberg S, Schuck P, Phillips TM, Andrews SB, Leapman RD | PLoS One | 10.1371/journal.pone.0087921 | 2014 | Carbohydrate Metabolism, Cell Membrane/ultrastructure, Cytoskeleton/ultrastructure, DNA/chemistry, Lipids/analysis, Microscopy, Electron, Scanning Transmission, Proteins/analysis, RNA/chemistry, Spiroplasma/*physiology/*ultrastructure, Ultracentrifugation |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 15979 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21833) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21833 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 83500 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID40399.1 | StrainInfo: A central database for resolving microbial strain identifiers |