Archive BacDiveID:10.13145/bacdive14353.20230509.8.1

Strain identifier

BacDive ID: 14353

Type strain:

Species: Spiroplasma citri

Strain Designation: Maroc, Morocco-R8-A2

Strain history: <- J. K. Davis; <- J. G. Tully; <- J. M. Bove; Morocco-R8-A2

NCBI tax ID(s): 2133 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 15987

BacDive-ID: 14353

DSM-Number: 21846

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, plant pathogen

description: Spiroplasma citri Maroc is a microaerophile, mesophilic plant pathogen that was isolated from stubborn disease-affected citrus plant.

NCBI tax id

NCBI tax id: 2133
Matching level: species

strain history

@ref: 15987
history: <- J. K. Davis; <- J. G. Tully; <- J. M. Bove; Morocco-R8-A2

doi: 10.13145/bacdive14353.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/mycoplasmatota
domain: Bacteria
phylum: Mycoplasmatota
class: Mollicutes
order: Mycoplasmatales
family: Spiroplasmataceae
genus: Spiroplasma
species: Spiroplasma citri
full scientific name: Spiroplasma citri Saglio et al. 1973 (Approved Lists 1980)

@ref: 15987

domain: Bacteria

phylum: Tenericutes

class: Mollicutes

order: Entomoplasmatales

family: Spiroplasmataceae

genus: Spiroplasma

species: Spiroplasma citri

full scientific name: Spiroplasma citri Saglio et al. 1973

strain designation: Maroc, Morocco-R8-A2

type strain: yes

Morphology

colony morphology

@ref: 15987
incubation period: 2-3 days

Culture and growth conditions

culture medium

@ref: 15987
name: SP4-Z MEDIUM (DSMZ Medium 1076b)
growth: yes
link: https://mediadive.dsmz.de/medium/1076b
composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water

culture temp

@ref: 15987
growth: positive
type: growth
temperature: 30
range: mesophilic

Physiology and metabolism

oxygen tolerance

@ref: 15987
oxygen tolerance: microaerophile

Isolation, sampling and environmental information

isolation

@ref: 15987
sample type: stubborn disease-affected citrus plant
country: Morocco
origin.country: MAR
continent: Africa

isolation source categories

Cat1	Cat2	Cat3
#Infection
#Host	#Plants	#Shrub (Scrub)
#Host	#Plants	#Tree

Safety information

risk assessment

@ref: 15987
pathogenicity plant: yes
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Spiroplasma citri 16S/23S rRNA spacer region, complete sequence	AF005327	299	ena	2133
20218	Spiroplasma citri 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY729936	914	ena	2133
15987	S.citri 16S ribosomal RNA small subunit	M23942	1523	ena	2133

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Spiroplasma citri strain R8-A2	2133.28	plasmid	patric	2133
66792	Spiroplasma citri strain R8-A2	2133.18	complete	patric	2133
66792	Spiroplasma citri strain R8-A2	2133.27	plasmid	patric	2133

Genome-based predictions

predictions

trait	prediction	confidence	training_data
motile	no	92.719	no
gram-positive	yes	70.864	no
anaerobic	no	93.358	yes
aerobic	no	97.922	yes
halophile	yes	64.955	no
spore-forming	no	98.436	no
glucose-util	yes	89.574	no
flagellated	no	98.072	no
thermophile	no	93.808	yes
glucose-ferment	yes	73.817	no

External links

@ref: 15987

culture collection no.: DSM 21846, ATCC 27556, NCPPB 2647

straininfo link

@ref: 83496
straininfo: 46663

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	93504	Spiroplasmas: serological grouping of strains associated with plants and insects.	Davis RE, Lee IM, Basciano LK	Can J Microbiol	10.1139/m79-128	1979	Animals, Epitopes, Insecta/microbiology, Plants/microbiology, Precipitin Tests, Spiroplasma/*classification
Phylogeny	2830894	Spiroplasma species share common DNA sequences among their viruses, plasmids and genomes.	Ranhand JM, Nur I, Rose DL, Tully JG	Ann Inst Pasteur Microbiol	10.1016/0769-2609(87)90037-8	1987	Bacteriophages/genetics, Base Sequence, DNA Restriction Enzymes, DNA, Bacterial/genetics, DNA, Viral/genetics, Genes, Bacterial, Nucleic Acid Hybridization, Plasmids, Sequence Homology, Nucleic Acid, Spiroplasma/*genetics	Enzymology
Enzymology	3039555	Short, interspersed, and repetitive DNA sequences in Spiroplasma species.	Nur I, LeBlanc DJ, Tully JG	Plasmid	10.1016/0147-619x(87)90017-5	1987	Chromosome Mapping, Chromosomes, Bacterial/physiology, Cloning, Molecular, DNA Restriction Enzymes, Repetitive Sequences, Nucleic Acid, Spiroplasma/*genetics
Phylogeny	6201097	Comparison of the amino acid compositions and antigenic properties of spiralins purified from the plasma membranes of different spiroplasmas.	Wroblewski H, Robic D, Thomas D, Blanchard A	Ann Microbiol (Paris)	10.1016/s0769-2609(84)80061-7	1984	Amino Acids/analysis, Antigens, Bacterial/analysis, Bacterial Outer Membrane Proteins, Bacterial Proteins/analysis/immunology, Cell Membrane/analysis, Epitopes/analysis, Membrane Proteins/analysis/immunology, Spiroplasma/*classification/immunology
Pathogenicity	6830208	In vitro susceptibility of spiroplasmas to heavy-metal salts.	Whitmore SC, Rissler JF, Davis RE	Antimicrob Agents Chemother	10.1128/AAC.23.1.22	1983	Hydrogen-Ion Concentration, Metals/pharmacology, Microbial Sensitivity Tests, Spiroplasma/drug effects
Enzymology	7214225	DNA homology among diverse spiroplasma strains representing several serological groups.	Lee IM, Davis RE	Can J Microbiol	10.1139/m80-224	1980	DNA, Bacterial/analysis/genetics, Kinetics, Nucleic Acid Renaturation, Serotyping, Spiroplasma/classification/genetics/immunology	Phylogeny
Cultivation	16346437	Chemically defined medium for cultivation of several epiphytic and phytopathogenic spiroplasmas.	Lee IM, Davis RE	Appl Environ Microbiol	10.1128/aem.46.6.1247-1251.1983	1983
Pathogenicity	16535161	Resistance of Spiroplasma citri Lines to the Virus SVTS2 Is Associated with Integration of Viral DNA Sequences into Host Chromosomal and Extrachromosomal DNA.	Sha Y, Melcher U, Davis RE, Fletcher J	Appl Environ Microbiol	10.1128/aem.61.11.3950-3959.1995	1995

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
15987	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21846)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-21846
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
83496	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID46663.1	StrainInfo: A central database for resolving microbial strain identifiers