Strain identifier

BacDive ID: 14345

Type strain: Yes

Species: Treponema azotonutricium

Strain Designation: ZAS-9

Strain history: <- J. A. Breznak, Michigan State University, Center for Microbial Ecology, E. Lansing, USA; ZAS-9 <- J. R. Leadbetter and J. A. Breznak {1998}

NCBI tax ID(s): 150829 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5177

BacDive-ID: 14345

DSM-Number: 13862

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, psychrophilic

description: Treponema azotonutricium ZAS-9 is an anaerobe, psychrophilic bacterium that was isolated from hindgut of wood-eating termites .

NCBI tax id

  • NCBI tax id: 150829
  • Matching level: species

strain history

  • @ref: 5177
  • history: <- J. A. Breznak, Michigan State University, Center for Microbial Ecology, E. Lansing, USA; ZAS-9 <- J. R. Leadbetter and J. A. Breznak {1998}

doi: 10.13145/bacdive14345.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Spirochaetales
  • family: Treponemataceae
  • genus: Treponema
  • species: Treponema azotonutricium
  • full scientific name: Treponema azotonutricium Graber et al. 2004
  • synonyms

    • @ref: 20215
    • synonym: Leadbettera azotonutricia

@ref: 5177

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Treponemataceae

genus: Treponema

species: Treponema azotonutricium

full scientific name: Treponema azotonutricium Graber et al. 2004

strain designation: ZAS-9

type strain: yes

Morphology

multimedia

  • @ref: 5177
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_13862.jpg
  • caption: Medium 169, 20°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

  • @ref: 5177
  • name: LEADBETTERA MEDIUM (DSMZ Medium 169b)
  • growth: yes
  • link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium169b.pdf

culture temp

  • @ref: 5177
  • growth: positive
  • type: growth
  • temperature: 20
  • range: psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 5177
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 5177
  • sample type: hindgut of wood-eating termites (Zootermopsis angusticollis)
  • host species: Zootermopsis angusticollis
  • geographic location: California
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host#Plants
#Host Body-Site#Gastrointestinal tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_7366.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_613;96_3447;97_4229;98_5402;99_7366&stattab=map
  • Last taxonomy: Treponema azotonutricium subclade
  • 16S sequence: AF320287
  • Sequence Identity:
  • Total samples: 191
  • soil counts: 20
  • aquatic counts: 48
  • animal counts: 114
  • plant counts: 9

Safety information

risk assessment

  • @ref: 5177
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5177
  • description: Treponema sp. ZAS-9 16S ribosomal RNA gene, partial sequence
  • accession: AF320287
  • length: 1463
  • database: ena
  • NCBI tax ID: 545695

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Leadbettera azotonutricia ZAS-9GCA_000214355completencbi545695
66792Treponema azotonutricium ZAS-9545695.3completepatric545695
66792Treponema azotonutricium ZAS-9650716099completeimg545695

GC content

  • @ref: 5177
  • GC-content: 50

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes82.077no
flagellatedno75.585no
gram-positiveno91.948no
anaerobicyes98.841yes
aerobicno97.914no
halophileno93.729no
spore-formingno85.176no
thermophileno82.665yes
glucose-utilyes85.452no
glucose-fermentyes68.577no

External links

@ref: 5177

culture collection no.: DSM 13862, ATCC BAA 888

straininfo link

  • @ref: 83490
  • straininfo: 132032

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15006748Description of Treponema azotonutricium sp. nov. and Treponema primitia sp. nov., the first spirochetes isolated from termite guts.Graber JR, Leadbetter JR, Breznak JAAppl Environ Microbiol10.1128/AEM.70.3.1315-1320.20042004Animals, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Digestive System/microbiology, Fermentation, Genome, Bacterial, Isoptera/*microbiology, Microscopy, Electron, Molecular Sequence Data, Phenotype, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Treponema/classification/genetics/*isolation & purification/metabolismGenetics
Metabolism21811792Genomic analysis reveals multiple [FeFe] hydrogenases and hydrogen sensors encoded by treponemes from the H(2)-rich termite gut.Ballor NR, Paulsen I, Leadbetter JRMicrob Ecol10.1007/s00248-011-9922-82011Animals, Bacterial Proteins/*genetics, Formate Dehydrogenases/genetics/metabolism, Gastrointestinal Tract/metabolism/microbiology, Hydrogen/metabolism, Hydrogenase/*genetics, Isoptera/metabolism/*microbiology, Molecular Sequence Data, Multigene Family, Phylogeny, Polymerase Chain Reaction, Sequence Analysis, DNA, Treponema/classification/enzymology/*genetics/metabolismEnzymology

Reference

@idauthorscataloguedoi/urltitle
5177Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13862)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13862
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83490Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID132032.1StrainInfo: A central database for resolving microbial strain identifiers