Strain identifier
BacDive ID: 14345
Type strain: 
Species: Treponema azotonutricium
Strain Designation: ZAS-9
Strain history: <- J. A. Breznak, Michigan State University, Center for Microbial Ecology, E. Lansing, USA; ZAS-9 <- J. R. Leadbetter and J. A. Breznak; {1998}
NCBI tax ID(s): 150829 (species)
version 9.3 (current version)
General
@ref: 5177
BacDive-ID: 14345
DSM-Number: 13862
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, psychrophilic
description: Treponema azotonutricium ZAS-9 is an anaerobe, psychrophilic bacterium that was isolated from hindgut of wood-eating termites .
NCBI tax id
- NCBI tax id: 150829
- Matching level: species
strain history
- @ref: 5177
- history: <- J. A. Breznak, Michigan State University, Center for Microbial Ecology, E. Lansing, USA; ZAS-9 <- J. R. Leadbetter and J. A. Breznak; {1998}
doi: 10.13145/bacdive14345.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/spirochaetota
- domain: Bacteria
- phylum: Spirochaetota
- class: Spirochaetia
- order: Spirochaetales
- family: Treponemataceae
- genus: Treponema
- species: Treponema azotonutricium
- full scientific name: Treponema azotonutricium Graber et al. 2004
synonyms
- @ref: 20215
- synonym: Leadbettera azotonutricia
@ref: 5177
domain: Bacteria
phylum: Spirochaetes
class: Spirochaetia
order: Spirochaetales
family: Treponemataceae
genus: Treponema
species: Treponema azotonutricium
full scientific name: Treponema azotonutricium Graber et al. 2004
strain designation: ZAS-9
type strain: yes
Morphology
cell morphology
- @ref: 125439
- gram stain: negative
- confidence: 96.5
multimedia
- @ref: 5177
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_13862.jpg
- caption: Medium 169b, 20°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
- @ref: 5177
- name: LEADBETTERA MEDIUM (DSMZ Medium 169b)
- growth: yes
- link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium169b.pdf
culture temp
- @ref: 5177
- growth: positive
- type: growth
- temperature: 20
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 5177 | anaerobe | |
| 125438 | anaerobe | 93.997 |
| 125439 | anaerobe | 99.8 |
spore formation
- @ref: 125439
- spore formation: no
- confidence: 92.6
Isolation, sampling and environmental information
isolation
- @ref: 5177
- sample type: hindgut of wood-eating termites (Zootermopsis angusticollis)
- host species: Zootermopsis angusticollis
- geographic location: California
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Arthropoda | #Insecta |
| #Host | #Plants | |
| #Host Body-Site | #Gastrointestinal tract |
taxonmaps
- @ref: 69479
- File name: preview.99_7366.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_613;96_3447;97_4229;98_5402;99_7366&stattab=map
- Last taxonomy: Treponema azotonutricium subclade
- 16S sequence: AF320287
- Sequence Identity:
- Total samples: 191
- soil counts: 20
- aquatic counts: 48
- animal counts: 114
- plant counts: 9
Safety information
risk assessment
- @ref: 5177
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5177
- description: Treponema sp. ZAS-9 16S ribosomal RNA gene, partial sequence
- accession: AF320287
- length: 1463
- database: nuccore
- NCBI tax ID: 545695
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Leadbettera azotonutricia ZAS-9 | GCA_000214355 | complete | ncbi | 545695 |
| 66792 | Treponema azotonutricium ZAS-9 | 545695.3 | complete | patric | 545695 |
| 66792 | Treponema azotonutricium ZAS-9 | 650716099 | complete | img | 545695 |
GC content
- @ref: 5177
- GC-content: 50
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 82.908 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 93.997 | yes |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 93.643 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 76.637 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 83.029 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | yes | 57.018 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | no | 92.6 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | no | 65.6 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | negative | 96.5 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | anaerobe | 99.8 |
External links
@ref: 5177
culture collection no.: DSM 13862, ATCC BAA-888
straininfo link
- @ref: 83490
- straininfo: 132032
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 15006748 | Description of Treponema azotonutricium sp. nov. and Treponema primitia sp. nov., the first spirochetes isolated from termite guts. | Graber JR, Leadbetter JR, Breznak JA | Appl Environ Microbiol | 10.1128/AEM.70.3.1315-1320.2004 | 2004 | Animals, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Digestive System/microbiology, Fermentation, Genome, Bacterial, Isoptera/*microbiology, Microscopy, Electron, Molecular Sequence Data, Phenotype, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Treponema/classification/genetics/*isolation & purification/metabolism | Genetics |
| Metabolism | 21811792 | Genomic analysis reveals multiple [FeFe] hydrogenases and hydrogen sensors encoded by treponemes from the H(2)-rich termite gut. | Ballor NR, Paulsen I, Leadbetter JR | Microb Ecol | 10.1007/s00248-011-9922-8 | 2011 | Animals, Bacterial Proteins/*genetics, Formate Dehydrogenases/genetics/metabolism, Gastrointestinal Tract/metabolism/microbiology, Hydrogen/metabolism, Hydrogenase/*genetics, Isoptera/metabolism/*microbiology, Molecular Sequence Data, Multigene Family, Phylogeny, Polymerase Chain Reaction, Sequence Analysis, DNA, Treponema/classification/enzymology/*genetics/metabolism | Enzymology |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5177 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13862) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13862 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 83490 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID132032.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | |
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |